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AB188582

Anti-U2AF1L4 antibody [EPR14349]

4

(1 Review)

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(2 Publications)

Rabbit Recombinant Monoclonal U2AF1L4 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 2 publications.

View Alternative Names

U2AF1-RS3, U2AF1L3, U2AF1L4, Splicing factor U2AF 26 kDa subunit, U2 auxiliary factor 26, U2 small nuclear RNA auxiliary factor 1-like protein 4, U2(RNU2) small nuclear RNA auxiliary factor 1-like protein 3, U2AF1-like 4, U2 small nuclear RNA auxiliary factor 1-like protein 3, U2AF1-like protein 3

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-U2AF1L4 antibody [EPR14349] (AB188582)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-U2AF1L4 antibody [EPR14349] (AB188582)

Immunofluorescent analysis of paraformaldehyde-fixed U87-MG cells labeling U2AF1L4 with ab188582 at 1/250 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) and DAPI staining.

Flow Cytometry (Intracellular) - Anti-U2AF1L4 antibody [EPR14349] (AB188582)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-U2AF1L4 antibody [EPR14349] (AB188582)

ab188582 staining U2AF1L4 in A549 (human lung carcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. The sample was incubated with the primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

Isoytype control : Rabbit monoclonal IgG (Black)

Unlabelled control : Cell without incubation with primary antibody and secondary antibody (Blue)

Western blot - Anti-U2AF1L4 antibody [EPR14349] (AB188582)
  • WB

Supplier Data

Western blot - Anti-U2AF1L4 antibody [EPR14349] (AB188582)

All lanes:

Western blot - Anti-U2AF1L4 antibody [EPR14349] (ab188582) at 1/10000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

Human cerebellum lysate at 20 µg

Lane 3:

K562 lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

false

Western blot - Anti-U2AF1L4 antibody [EPR14349] (AB188582)
  • WB

Supplier Data

Western blot - Anti-U2AF1L4 antibody [EPR14349] (AB188582)

All lanes:

Western blot - Anti-U2AF1L4 antibody [EPR14349] (ab188582)

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

PC-12 lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

false

  • Carrier free

    Anti-U2AF1L4 antibody [EPR14349] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14349

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

U2AF1L4 also known as U2 small nuclear RNA auxiliary factor 1-like 4 is a protein with a mass of about 30 kilodaltons. It plays a role in RNA splicing acting at the early stages of spliceosome assembly. U2AF1L4 binds to the polypyrimidine tract of pre-mRNA introns facilitating the recruitment of U2 snRNP to the branch point sequence. Expression of U2AF1L4 occurs mainly in tissues that are rich in nucleic acids and RNA such as testis and certain types of cancer cells.
Biological function summary

U2AF1L4 supports the regulation of gene expression through its role in the spliceosome machinery. It may form complexes with other splicing factors such as U2AF2 that are part of the early stages of spliceosomal assembly. This action supports the precise removal of introns and positions the exons accurately for translation. The ability to differentiate splice sites ensures diversity in mRNA transcription which is essential for proper function of genes in diverse tissues.

Pathways

U2AF1L4 fits into the pre-mRNA splicing pathway. It operates closely with other splicing-associated proteins like SF1 to help in the selection and cleavage of splice sites. Additionally U2AF1L4 interacts within the spliceosome pathway contributing to the splicing outcomes that regulate gene expression and are necessary for maintaining normal cell growth and function.

Irregularities in U2AF1L4 have been linked to certain cancers. Aberrant splicing by U2AF1L4 might play a role in tumor progression where mutations in its coding genes could lead to mis-splicing events. It engages with other proteins implicated in cancer such as the splicing regulator SF3B1 suggesting a complex interplay in tumorigenesis. Understanding these relationships could help develop targeted therapies for related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding protein that function as a pre-mRNA splicing factor. Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Acts by enhancing the binding of U2AF2 to weak pyrimidine tracts. Also participates in the regulation of alternative pre-mRNA splicing. Activates exon 5 skipping of PTPRC during T-cell activation; an event reversed by GFI1. Binds to RNA at the AG dinucleotide at the 3'-splice site (By similarity). Shows a preference for AGC or AGA (By similarity).
See full target information U2AF1L4
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

EMBO molecular medicine 15:e17973 PubMed37800682

2023

Trichothiodystrophy-associated MPLKIP maintains DBR1 levels for proper lariat debranching and ectodermal differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Arjan F Theil,Alex Pines,Tuğba Kalayci,José M Heredia-Genestar,Anja Raams,Marion H Rietveld,Sriram Sridharan,Sabine Ej Tanis,Klaas W Mulder,Nesimi Büyükbabani,Birsen Karaman,Zehra O Uyguner,Hülya Kayserili,Jan Hj Hoeijmakers,Hannes Lans,Jeroen Aa Demmers,Joris Pothof,Umut Altunoglu,Abdoelwaheb El Ghalbzouri,Wim Vermeulen

Cellular and molecular neurobiology 43:1199-1218 PubMed35748966

2022

LncRNA Pnky Positively Regulates Neural Stem Cell Migration by Modulating mRNA Splicing and Export of Target Genes.

Applications

Unspecified application

Species

Unspecified reactive species

Jiannan Du,Yuan Li,Yuting Su,Wenqian Zhi,Jiale Zhang,Cheng Zhang,Juan Wang,Wensheng Deng,Shasha Zhao
View all publications
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