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AB86305

Anti-U2AF35/U2AF1 antibody

4

(1 Review)

|

(11 Publications)

Rabbit Polyclonal U2AF35/U2AF1 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human U2AF1 aa 100-200.

View Alternative Names

U2AF35, U2AFBP, FP793, U2AF1, Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor 35 kDa subunit, U2 small nuclear RNA auxiliary factor 1, U2 snRNP auxiliary factor small subunit

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody (AB86305)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody (AB86305)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma (left) and mouse CT26 colon carcinoma (right) tissues labelling U2AF35 with ab86305 at 1/200 (1µg/ml). Detection : DAB.

Immunoprecipitation - Anti-U2AF35/U2AF1 antibody (AB86305)
  • IP

Unknown

Immunoprecipitation - Anti-U2AF35/U2AF1 antibody (AB86305)

Detection of U2AF35/U2AF1 by Western Blot of Immunprecipitate.
ab86305, at 1 μg/ml, staining U2AF35/U2AF1 in HeLa whole cell lysate immunoprecipitated using ab86305 at 3 μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-U2AF35/U2AF1 antibody (ab86305)

Predicted band size: 28 kDa

false

Western blot - Anti-U2AF35/U2AF1 antibody (AB86305)
  • WB

Unknown

Western blot - Anti-U2AF35/U2AF1 antibody (AB86305)

All lanes:

Western blot - Anti-U2AF35/U2AF1 antibody (ab86305) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 28 kDa

Observed band size: 36 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP, IHC-P

applications

Immunogen

Synthetic Peptide within Human U2AF1 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q01081

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab86305 was affinity purified using an epitope specific to U2AF35/U2AF1 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

U2AF35 also known as U2AF1 is a protein weighing approximately 35 kDa. It interacts importantly in the pre-mRNA splicing process where it binds to the 3' splice site of introns. The protein is a subunit of the U2 auxiliary factor and its expression occurs across various tissues with particular concentrations in the developmental stages of tissues and in dynamic cellular environments. U2AF35 functions by recognizing the AG dinucleotide at the 3' end of introns a task critical for the accurate splicing of precursor mRNA.
Biological function summary

This protein plays an important role in the removal of introns from pre-mRNA impacting the processing of mRNA which ultimately affects gene expression. U2AF35 is a component of the spliceosome complex particularly influencing the recruitment of U2 small nuclear ribonucleoprotein (snRNP) to the branch point. The activity of U2AF35 ensures proper mRNA maturation which is essential in controlling the flow of genetic information from DNA to protein synthesis. It also contributes to the alternative splicing allowing cells to produce diverse protein isoforms from a single gene.

Pathways

U2AF35 is critically involved in the mRNA splicing pathway and the regulation of alternative splicing events. U2AF35 partners with another subunit U2AF65 in the spliceosome to facilitate these processes. It actively connects with several other proteins within the spliceosome machinery including SF3b and SF3a complexes. The interaction between U2AF35 and these elements ensures the correct assembly of the spliceosome directly influencing the pathway governing pre-mRNA processing.

Mutations in the U2AF35 gene can result in specific types of cancer such as myelodysplastic syndromes (MDS) where abnormal splicing patterns occur. Such mutations often affect the interaction of U2AF35 with other splicing factors like SF3B1. Additionally alterations in U2AF35 have associations with lung adenocarcinoma where changes in the mRNA splicing landscape empower cancer progression and malignancy. Understanding its role in splicing provides insight into how disruptions can lead to disease development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.
See full target information U2AF1
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 84:2618-2633.e10 PubMed39025073

2024

The splicing factor CCAR1 regulates the Fanconi anemia/BRCA pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Naoya Harada,Shuhei Asada,Lige Jiang,Huy Nguyen,Lisa Moreau,Ryan J Marina,Karen Adelman,Divya R Iyer,Alan D D'Andrea

Proceedings of the National Academy of Sciences of the United States of America 121:e2306814121 PubMed38513102

2024

Activation of ERβ hijacks the splicing machinery to trigger R-loop formation in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Dongfang Wang,Muya Tang,Peidong Zhang,Kailin Yang,Liang Huang,Mengrui Wu,Qiuhong Shen,Jing Yue,Wei Wang,Yanqiu Gong,Margaret Warner,Lunzhi Dai,Haihuai He,Zhengnan Yang,Jan-Ake Gustafsson,Shengtao Zhou

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed33876749

2021

Alternative splicing redefines landscape of commonly mutated genes in acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Osvaldo D Rivera,Michael J Mallory,Mathieu Quesnel-Vallières,Rakesh Chatrikhi,David C Schultz,Martin Carroll,Yoseph Barash,Sara Cherry,Kristen W Lynch

Cell chemical biology 28:1145-1157.e6 PubMed33689684

2021

A synthetic small molecule stalls pre-mRNA splicing by promoting an early-stage U2AF2-RNA complex.

Applications

Unspecified application

Species

Unspecified reactive species

Rakesh Chatrikhi,Callen F Feeney,Mary J Pulvino,Georgios Alachouzos,Andrew J MacRae,Zackary Falls,Sumit Rai,William W Brennessel,Jermaine L Jenkins,Matthew J Walter,Timothy A Graubert,Ram Samudrala,Melissa S Jurica,Alison J Frontier,Clara L Kielkopf

Aging cell 19:e13276 PubMed33274830

2020

Prevalent intron retention fine-tunes gene expression and contributes to cellular senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Yao,Dong Ding,Xueping Li,Ting Shen,Haihui Fu,Hua Zhong,Gang Wei,Ting Ni

Nucleic acids research 47:10373-10387 PubMed31504847

2019

mTOR-regulated U2af1 tandem exon splicing specifies transcriptome features for translational control.

Applications

Unspecified application

Species

Unspecified reactive species

Jae-Woong Chang,Hsin-Sung Yeh,Meeyeon Park,Luke Erber,Jiao Sun,Sze Cheng,Alexander M Bui,Naima Ahmed Fahmi,Ryan Nasti,Rui Kuang,Yue Chen,Wei Zhang,Jeongsik Yong

Nature cell biology 21:640-650 PubMed31011167

2019

U2AF1 mutations induce oncogenic IRAK4 isoforms and activate innate immune pathways in myeloid malignancies.

Applications

Unspecified application

Species

Unspecified reactive species

Molly A Smith,Gaurav S Choudhary,Andrea Pellagatti,Kwangmin Choi,Lyndsey C Bolanos,Tushar D Bhagat,Shanisha Gordon-Mitchell,Dagny Von Ahrens,Kith Pradhan,Violetta Steeples,Sanghyun Kim,Ulrich Steidl,Matthew Walter,Iain D C Fraser,Aishwarya Kulkarni,Nathan Salomonis,Kakajan Komurov,Jacqueline Boultwood,Amit Verma,Daniel T Starczynowski

Nucleic acids research 46:6166-6187 PubMed29788428

2018

PUF60-activated exons uncover altered 3' splice-site selection by germline missense mutations in a single RRM.

Applications

Unspecified application

Species

Unspecified reactive species

Jana Královicová,Ivana Ševcíková,Eva Stejskalová,Mina Obuca,Michael Hiller,David Stanek,Igor Vorechovský

Genes & development 29:2054-66 PubMed26443849

2015

Widespread JNK-dependent alternative splicing induces a positive feedback loop through CELF2-mediated regulation of MKK7 during T-cell activation.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole M Martinez,Laura Agosto,Jinsong Qiu,Michael J Mallory,Matthew R Gazzara,Yoseph Barash,Xiang-Dong Fu,Kristen W Lynch

Proceedings of the National Academy of Sciences of 112:E4726-34 PubMed26261309

2015

Disease-associated mutation in SRSF2 misregulates splicing by altering RNA-binding affinities.

Applications

WB

Species

Unspecified reactive species

Jian Zhang,Yen K Lieu,Abdullah M Ali,Alex Penson,Kathryn S Reggio,Raul Rabadan,Azra Raza,Siddhartha Mukherjee,James L Manley
View all publications
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