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Rabbit Recombinant Monoclonal U2AF35/U2AF1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Rat, Human samples. Cited in 1 publication.

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Images

Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (AB197591), expandable thumbnail
  • Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (AB197591), expandable thumbnail
  • Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (AB197591), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (AB197591), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (AB197591), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Predicted
Rat
Tested
Expected
Expected
Tested

Tested
Tested

Species
Rat
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/170
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.

Alternative names

U2AF35, U2AFBP, FP793, U2AF1, Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor 35 kDa subunit, U2 small nuclear RNA auxiliary factor 1, U2 snRNP auxiliary factor small subunit

Recommended products

Rabbit Recombinant Monoclonal U2AF35/U2AF1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Rat, Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR12648(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

U2AF35 also known as U2AF1 is a protein weighing approximately 35 kDa. It interacts importantly in the pre-mRNA splicing process where it binds to the 3' splice site of introns. The protein is a subunit of the U2 auxiliary factor and its expression occurs across various tissues with particular concentrations in the developmental stages of tissues and in dynamic cellular environments. U2AF35 functions by recognizing the AG dinucleotide at the 3' end of introns a task critical for the accurate splicing of precursor mRNA.

Biological function summary

This protein plays an important role in the removal of introns from pre-mRNA impacting the processing of mRNA which ultimately affects gene expression. U2AF35 is a component of the spliceosome complex particularly influencing the recruitment of U2 small nuclear ribonucleoprotein (snRNP) to the branch point. The activity of U2AF35 ensures proper mRNA maturation which is essential in controlling the flow of genetic information from DNA to protein synthesis. It also contributes to the alternative splicing allowing cells to produce diverse protein isoforms from a single gene.

Pathways

U2AF35 is critically involved in the mRNA splicing pathway and the regulation of alternative splicing events. U2AF35 partners with another subunit U2AF65 in the spliceosome to facilitate these processes. It actively connects with several other proteins within the spliceosome machinery including SF3b and SF3a complexes. The interaction between U2AF35 and these elements ensures the correct assembly of the spliceosome directly influencing the pathway governing pre-mRNA processing.

Associated diseases and disorders

Mutations in the U2AF35 gene can result in specific types of cancer such as myelodysplastic syndromes (MDS) where abnormal splicing patterns occur. Such mutations often affect the interaction of U2AF35 with other splicing factors like SF3B1. Additionally alterations in U2AF35 have associations with lung adenocarcinoma where changes in the mRNA splicing landscape empower cancer progression and malignancy. Understanding its role in splicing provides insight into how disruptions can lead to disease development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591), expandable thumbnail

    Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)

    Blocking/dilution buffer: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature PMID:1388271.

    All lanes: Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591) at 1/10000 dilution

    All lanes: Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 28 kDa

    Observed band size: 35 kDa

  • Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591), expandable thumbnail

    Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)

    Blocking/dilution buffer: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature PMID:1388271.

    All lanes: Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591) at 1/20000 dilution

    Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

    Lane 2: 293 (Human embryonic kidney) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 28 kDa

    Observed band size: 35 kDa

  • Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591), expandable thumbnail

    Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)

    Blocking/dilution buffer: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature PMID:1388271

    All lanes: Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591) at 1/1000 dilution

    All lanes: Rat spleen lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 28 kDa

    Observed band size: 35 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling U2AF35/U2AF1 with ab197591 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cytoplasm and nuclear staining on Human transitional cell carcinoma of bladder tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling U2AF35/U2AF1 with ab197591 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP)(Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cytoplasm and nuclear staining on rat liver tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)

    Immunocytochemistry/Immunofluorescence analysis of RAMOS cells labelling U2AF35/U2AF1 with ab197591 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Flow Cytometry (Intracellular) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Ramos (Human Burkitt's lymphoma)cells labeling U2AF35/U2AF1 with ab197591 at 1/170 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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