Rabbit Recombinant Monoclonal U2AF35/U2AF1 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Rat samples.
Images
![Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (AB251242), expandable thumbnail](https://content.abcam.com/products/images/u2af35-u2af1-antibody-epr126482-bsa-and-azide-free-ab251242--western-blot-img155710.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (AB251242)")
![Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (AB251242), expandable thumbnail](https://content.abcam.com/products/images/u2af35-u2af1-antibody-epr126482-bsa-and-azide-free-ab251242--western-blot-img146359.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (AB251242)")
![Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (AB251242), expandable thumbnail](https://content.abcam.com/products/images/u2af35-u2af1-antibody-epr126482-bsa-and-azide-free-ab251242--western-blot-img145122.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (AB251242)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (AB251242), expandable thumbnail](https://content.abcam.com/products/images/u2af35-u2af1-antibody-epr126482-bsa-and-azide-free-ab251242--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134730.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (AB251242)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (AB251242), expandable thumbnail](https://content.abcam.com/products/images/u2af35-u2af1-antibody-epr126482-bsa-and-azide-free-ab251242--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113191.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (AB251242)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted |
| Rat | Tested | Tested | Expected | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.
Alternative names
U2AF35, U2AFBP, FP793, U2AF1, Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor 35 kDa subunit, U2 small nuclear RNA auxiliary factor 1, U2 snRNP auxiliary factor small subunit
Recommended products
Rabbit Recombinant Monoclonal U2AF35/U2AF1 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR12648(2)
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251242 is the carrier-free version of Anti-U2AF35/U2AF1 antibody [EPR12648(2)] ab197591.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
U2AF35 also known as U2AF1 is a protein weighing approximately 35 kDa. It interacts importantly in the pre-mRNA splicing process where it binds to the 3' splice site of introns. The protein is a subunit of the U2 auxiliary factor and its expression occurs across various tissues with particular concentrations in the developmental stages of tissues and in dynamic cellular environments. U2AF35 functions by recognizing the AG dinucleotide at the 3' end of introns a task critical for the accurate splicing of precursor mRNA.
- Biological function summary
This protein plays an important role in the removal of introns from pre-mRNA impacting the processing of mRNA which ultimately affects gene expression. U2AF35 is a component of the spliceosome complex particularly influencing the recruitment of U2 small nuclear ribonucleoprotein (snRNP) to the branch point. The activity of U2AF35 ensures proper mRNA maturation which is essential in controlling the flow of genetic information from DNA to protein synthesis. It also contributes to the alternative splicing allowing cells to produce diverse protein isoforms from a single gene.
- Pathways
U2AF35 is critically involved in the mRNA splicing pathway and the regulation of alternative splicing events. U2AF35 partners with another subunit U2AF65 in the spliceosome to facilitate these processes. It actively connects with several other proteins within the spliceosome machinery including SF3b and SF3a complexes. The interaction between U2AF35 and these elements ensures the correct assembly of the spliceosome directly influencing the pathway governing pre-mRNA processing.
- Associated diseases and disorders
Mutations in the U2AF35 gene can result in specific types of cancer such as myelodysplastic syndromes (MDS) where abnormal splicing patterns occur. Such mutations often affect the interaction of U2AF35 with other splicing factors like SF3B1. Additionally alterations in U2AF35 have associations with lung adenocarcinoma where changes in the mRNA splicing landscape empower cancer progression and malignancy. Understanding its role in splicing provides insight into how disruptions can lead to disease development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242), expandable thumbnail](https://content.abcam.com/products/images/u2af35-u2af1-antibody-epr126482-bsa-and-azide-free-ab251242--western-blot-img155710.jpg "Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)")
Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)
This data was developed using Anti-U2AF35/U2AF1 antibody [EPR12648(2)] ab197591, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature (PMID: 1388271).
All lanes: Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (Anti-U2AF35/U2AF1 antibody [EPR12648(2)] ab197591) at 1/10000 dilution
All lanes: Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 35 kDa
![Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242), expandable thumbnail](https://content.abcam.com/products/images/u2af35-u2af1-antibody-epr126482-bsa-and-azide-free-ab251242--western-blot-img146359.jpg "Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)")
Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)
This data was developed using Anti-U2AF35/U2AF1 antibody [EPR12648(2)] ab197591, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature (PMID: 1388271).
All lanes: Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (Anti-U2AF35/U2AF1 antibody [EPR12648(2)] ab197591) at 1/20000 dilution
Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg
Lane 2: 293 (Human embryonic kidney) whole cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 35 kDa
![Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242), expandable thumbnail](https://content.abcam.com/products/images/u2af35-u2af1-antibody-epr126482-bsa-and-azide-free-ab251242--western-blot-img145122.jpg "Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)")
Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)
This data was developed using Anti-U2AF35/U2AF1 antibody [EPR12648(2)] ab197591, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature (PMID: 1388271).
All lanes: Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (Anti-U2AF35/U2AF1 antibody [EPR12648(2)] ab197591) at 1/1000 dilution
All lanes: Rat spleen lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 35 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242), expandable thumbnail](https://content.abcam.com/products/images/u2af35-u2af1-antibody-epr126482-bsa-and-azide-free-ab251242--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134730.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)
This data was developed using Anti-U2AF35/U2AF1 antibody [EPR12648(2)] ab197591, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling U2AF35/U2AF1 with Anti-U2AF35/U2AF1 antibody [EPR12648(2)] ab197591 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cytoplasm and nuclear staining on Human transitional cell carcinoma of bladder tissue is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242), expandable thumbnail](https://content.abcam.com/products/images/u2af35-u2af1-antibody-epr126482-bsa-and-azide-free-ab251242--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113191.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)
This data was developed using Anti-U2AF35/U2AF1 antibody [EPR12648(2)] ab197591, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling U2AF35/U2AF1 with Anti-U2AF35/U2AF1 antibody [EPR12648(2)] ab197591 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP)(Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cytoplasm and nuclear staining on rat liver tissue is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242), expandable thumbnail](https://content.abcam.com/products/images/u2af35-u2af1-antibody-epr126482-bsa-and-azide-free-ab251242--immunocytochemistry-immunofluorescence-img110201.jpg "Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)")
Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)
This data was developed using Anti-U2AF35/U2AF1 antibody [EPR12648(2)] ab197591, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of RAMOS cells labelling U2AF35/U2AF1 with Anti-U2AF35/U2AF1 antibody [EPR12648(2)] ab197591 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI.![Flow Cytometry (Intracellular) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242), expandable thumbnail](https://content.abcam.com/products/images/u2af35-u2af1-antibody-epr126482-bsa-and-azide-free-ab251242--flow-cytometry-intracellular-img37580.jpg "Flow Cytometry (Intracellular) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)")
Flow Cytometry (Intracellular) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)
This data was developed using Anti-U2AF35/U2AF1 antibody [EPR12648(2)] ab197591, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Ramos (Human Burkitt's lymphoma) cells labeling U2AF35/U2AF1 with Anti-U2AF35/U2AF1 antibody [EPR12648(2)] ab197591 at 1/170 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
![Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242), expandable thumbnail](https://content.abcam.com/products/images/u2af35-u2af1-antibody-epr126482-bsa-and-azide-free-ab251242--immunocytochemistry-immunofluorescence-img110201.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)")
![Flow Cytometry (Intracellular) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242), expandable thumbnail](https://content.abcam.com/products/images/u2af35-u2af1-antibody-epr126482-bsa-and-azide-free-ab251242--flow-cytometry-intracellular-img37580.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com