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Rabbit Recombinant Monoclonal U2AF65 antibody. C-terminal. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

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Images

Western blot - Anti-U2AF65 antibody [EPR17046] - C-terminal (AB197031), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-U2AF65 antibody [EPR17046] - C-terminal (AB197031), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF65 antibody [EPR17046] - C-terminal (AB197031), expandable thumbnail
  • Western blot - Anti-U2AF65 antibody [EPR17046] - C-terminal (AB197031), expandable thumbnail
  • Western blot - Anti-U2AF65 antibody [EPR17046] - C-terminal (AB197031), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Tested
Rat
Tested
Expected
Tested

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/450
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Plays a role in pre-mRNA splicing and 3'-end processing (PubMed:17024186). By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing (PubMed:21536736). Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10. Positively regulates pre-mRNA 3'-end processing by recruiting the CFIm complex to cleavage and polyadenylation signals (PubMed:17024186).

Alternative names

U2AF65, U2AF2, Splicing factor U2AF 65 kDa subunit, U2 auxiliary factor 65 kDa subunit, U2 snRNP auxiliary factor large subunit, hU2AF(65), hU2AF65

Recommended products

Rabbit Recombinant Monoclonal U2AF65 antibody. C-terminal. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17046
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

U2AF65 also known as U2 small nuclear RNA auxiliary factor 2 (U2AF2) is a splicing factor structured around 65 kilodaltons in mass. It interacts with RNA as part of the spliceosome to facilitate proper pre-mRNA splicing. The protein ensures the precise recognition and binding of the polypyrimidine tract at the 3' splice site. It is expressed in various human tissues playing an important role in RNA processing across different cell types.

Biological function summary

The U2AF65 protein contributes to the assembly of the spliceosome a major RNA-protein complex involved in splicing pre-messenger RNA. It works as part of the heterodimeric U2AF complex along with U2AF35 enhancing the precise selection of splice sites. This activity is essential to ensure accurate and efficient splicing which is necessary for proper gene expression and regulation.

Pathways

The activity of U2AF65 is an integral part of the RNA splicing pathway which plays a critical role in modulating gene expression. U2AF65 acts in coordination with other splicing factors such as SF1 and SF3B1 ensuring the correct assembly of the functional spliceosome. The accurate regulation of this pathway is essential for generating diversity in protein expression and for maintaining cell homeostasis.

Associated diseases and disorders

Misregulation of U2AF65 activity has implications in diseases like cancer and spinal muscular atrophy. Alterations in the expression or function of U2AF65 can lead to aberrant splicing events which affect gene expression profiles. In cancer U2AF65 interacts with various oncogenes and tumor suppressor proteins potentially contributing to tumour progression. In spinal muscular atrophy alterations in splicing factors including U2AF65 influence the expression of survival motor neuron proteins intricately linking U2AF65 to the pathology of these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031), expandable thumbnail

    Western blot - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031) at 1/20000 dilution

    Lane 1: 293 (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

    Lane 2: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

    Lane 3: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 54 kDa

    Observed band size: 54 kDa

    Exposure time: 1min

  • Immunocytochemistry/ Immunofluorescence - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling U2AF65 with Purified ab197031 at 1/500 dilution (5 μg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031)

    Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling U2AF65 with ab197031 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on Human endometrial adenocarcinoma tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031), expandable thumbnail

    Western blot - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031) at 1/2000 dilution

    Lane 1: C6 (Rat glial tumor cells) whole cell lysate at 10 µg

    Lane 2: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Lane 3: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

    Lane 4: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 54 kDa

    Observed band size: 54 kDa

    Exposure time: 1min

  • Western blot - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031), expandable thumbnail

    Western blot - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031) at 1/2000 dilution

    All lanes: Mouse brain tissue lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 54 kDa

    Observed band size: 54 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling U2AF65 with ab197031 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on Mouse liver tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031)

    Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling U2AF65 with ab197031 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on rat cerebral cortex tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

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