Rabbit Polyclonal UACA/Nucling antibody. Suitable for WB, IHC-P and reacts with Human, Recombinant full length protein - Human, Cow samples. Immunogen corresponding to Recombinant Fragment Protein within Human UACA aa 1-250.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human UACA aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BZF9
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Cow | Tested | Expected |
| Dog | Predicted | Predicted |
| Recombinant full length protein - Human | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.20000-2.00000 µg/mL | Notes - |
Species Recombinant full length protein - Human | Dilution info 0.20000-2.00000 µg/mL | Notes - |
Species Cow | Dilution info 0.20000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Dog | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5.00000-20.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Dog | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Target data
Function
Regulates APAF1 expression and plays an important role in the regulation of stress-induced apoptosis. Promotes apoptosis by regulating three pathways, apoptosome up-regulation, LGALS3/galectin-3 down-regulation and NF-kappa-B inactivation. Regulates the redistribution of APAF1 into the nucleus after proapoptotic stress. Down-regulates the expression of LGALS3 by inhibiting NFKB1 (By similarity). Modulates isoactin dynamics to regulate the morphological alterations required for cell growth and motility. Interaction with ARF6 may modulate cell shape and motility after injury. May be involved in multiple neurite formation (By similarity).
Alternative names
KIAA1561, UACA, Uveal autoantigen with coiled-coil domains and ankyrin repeats
Recommended products
Rabbit Polyclonal UACA/Nucling antibody. Suitable for WB, IHC-P and reacts with Human, Recombinant full length protein - Human, Cow samples. Immunogen corresponding to Recombinant Fragment Protein within Human UACA aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human UACA aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BZF9
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UACA also known as Nucling is a protein with a molecular mass of approximately 130 kDa. It shows expression in various tissues including the heart brain kidney and immune cells. Mechanically UACA participates in the regulation of inflammatory responses by interacting with members of the apoptosome complex. This protein plays a role in controlling programmed cell death known as apoptosis and it helps modulate immune signaling pathways.
- Biological function summary
UACA/Nucling contributes to cellular homeostasis. It forms part of a multi-protein complex interacting with APAF-1 influencing apoptosome assembly and function. UACA involvement in apoptosis makes it essential for maintaining equilibrium between cell survival and cell death an important factor in health and disease contexts. Its interaction with immune cells suggests a role in how the body responds to cellular stress and damage signals.
- Pathways
UACA/Nucling has significant involvement in the apoptotic and inflammatory signaling pathways. It collaborates with APAF-1 in the apoptosis pathway triggering cascade activation of caspases which are important for the execution of apoptosis. Furthermore UACA’s interaction with cytokine signaling pathways points to its regulatory effects on inflammation. Through these mechanisms UACA contributes considerably to immune responses and cellular turnover.
- Associated diseases and disorders
UACA/Nucling links to various pathological conditions including autoimmune diseases and certain cancers. Its dysregulation can disrupt normal apoptotic processes leading to uncontrolled cell survival often associated with cancer progression. Additionally anomalies in its function may contribute to autoimmune disorders through inappropriate immune activation. In these contexts UACA’s interaction with APAF-1 and caspase proteins becomes particularly relevant as they together modulate critical survival and apoptotic signals.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UACA/Nucling antibody (ab233432)
Formalin-fixed, paraffin-embedded human brain tissue stained for UACA/Nucling using ab233432 at 20 μg/mL in immunohistochemical analysis. DAB staining.
Western blot - Anti-UACA/Nucling antibody (ab233432)
All lanes: Western blot - Anti-UACA/Nucling antibody (ab233432) at 1 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2: Cow pancreas tissue
SecondaryAll lanes: HRP-Linked Guinea pig anti-rabbit at 1/1000 dilution
Predicted band size: 163 kDa
Western blot - Anti-UACA/Nucling antibody (ab233432)
All lanes: Western blot - Anti-UACA/Nucling antibody (ab233432) at 2 µg/mL
All lanes: Recombinant human UACA/Nucling protein
Predicted band size: 163 kDa
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