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AB233432

Anti-UACA/Nucling antibody

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(1 Publication)

Rabbit Polyclonal UACA/Nucling antibody. Suitable for WB, IHC-P and reacts with Human, Recombinant full length protein - Human, Cow samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human UACA aa 1-250.

View Alternative Names

KIAA1561, UACA, Uveal autoantigen with coiled-coil domains and ankyrin repeats

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UACA/Nucling antibody (AB233432)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UACA/Nucling antibody (AB233432)

Formalin-fixed, paraffin-embedded human brain tissue stained for UACA/Nucling using ab233432 at 20 μg/mL in immunohistochemical analysis. DAB staining.

Western blot - Anti-UACA/Nucling antibody (AB233432)
  • WB

Supplier Data

Western blot - Anti-UACA/Nucling antibody (AB233432)

All lanes:

Western blot - Anti-UACA/Nucling antibody (ab233432) at 2 µg/mL

All lanes:

Recombinant human UACA/Nucling protein

Predicted band size: 163 kDa

false

Western blot - Anti-UACA/Nucling antibody (AB233432)
  • WB

Supplier Data

Western blot - Anti-UACA/Nucling antibody (AB233432)

All lanes:

Western blot - Anti-UACA/Nucling antibody (ab233432) at 1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 2:

Cow pancreas tissue

Secondary

All lanes:

HRP-Linked Guinea pig anti-rabbit at 1/1000 dilution

Predicted band size: 163 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Cow, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human UACA aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q9BZF9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UACA also known as Nucling is a protein with a molecular mass of approximately 130 kDa. It shows expression in various tissues including the heart brain kidney and immune cells. Mechanically UACA participates in the regulation of inflammatory responses by interacting with members of the apoptosome complex. This protein plays a role in controlling programmed cell death known as apoptosis and it helps modulate immune signaling pathways.
Biological function summary

UACA/Nucling contributes to cellular homeostasis. It forms part of a multi-protein complex interacting with APAF-1 influencing apoptosome assembly and function. UACA involvement in apoptosis makes it essential for maintaining equilibrium between cell survival and cell death an important factor in health and disease contexts. Its interaction with immune cells suggests a role in how the body responds to cellular stress and damage signals.

Pathways

UACA/Nucling has significant involvement in the apoptotic and inflammatory signaling pathways. It collaborates with APAF-1 in the apoptosis pathway triggering cascade activation of caspases which are important for the execution of apoptosis. Furthermore UACA’s interaction with cytokine signaling pathways points to its regulatory effects on inflammation. Through these mechanisms UACA contributes considerably to immune responses and cellular turnover.

UACA/Nucling links to various pathological conditions including autoimmune diseases and certain cancers. Its dysregulation can disrupt normal apoptotic processes leading to uncontrolled cell survival often associated with cancer progression. Additionally anomalies in its function may contribute to autoimmune disorders through inappropriate immune activation. In these contexts UACA’s interaction with APAF-1 and caspase proteins becomes particularly relevant as they together modulate critical survival and apoptotic signals.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulates APAF1 expression and plays an important role in the regulation of stress-induced apoptosis. Promotes apoptosis by regulating three pathways, apoptosome up-regulation, LGALS3/galectin-3 down-regulation and NF-kappa-B inactivation. Regulates the redistribution of APAF1 into the nucleus after proapoptotic stress. Down-regulates the expression of LGALS3 by inhibiting NFKB1 (By similarity).. Modulates isoactin dynamics to regulate the morphological alterations required for cell growth and motility. Interaction with ARF6 may modulate cell shape and motility after injury. May be involved in multiple neurite formation (By similarity).
See full target information UACA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Tohoku journal of experimental medicine 264:9-19 PubMed38839357

2024

Shenqi Qiangjing Granules Ameliorate Asthenozoospermia in Mice by Regulating Ferroptosis through the METTL3/GPX4 Signaling Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuyu Lu,Jiabao Ma,Luying Wei,Jing Fu,Xiaoxia Li,Kedao Lai,Xin Li,Bingyu Xia,Bin Bin,Aicun Tang
View all publications
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Product promise

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