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AB314019

Anti-UAP1 antibody [1F2]

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Rabbit Recombinant Monoclonal UAP1 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human UAP1.

View Alternative Names

SPAG2, UAP1, UDP-N-acetylhexosamine pyrophosphorylase, Antigen X, Protein-pyrophosphorylation enzyme, Sperm-associated antigen 2, UDP-N-acetylgalactosamine pyrophosphorylase, UDP-N-acetylglucosamine pyrophosphorylase, AGX

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

1F2

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human UAP1.

Q16222

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UDP-N-acetylglucosamine pyrophosphorylase also known as UAP1 is an enzyme that catalyzes the formation of uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) from UTP and N-acetylglucosamine-1-phosphate. UAP1 has a mass of approximately 56 kDa. This enzyme is expressed in various tissues but shows higher expression levels in skeletal muscle heart and liver. In these tissues UAP1 plays a significant role in synthesizing nucleotides and certain sugars that are essential for other cellular processes.
Biological function summary

The role of UAP1 is central to the biosynthetic pathway of complex carbohydrates. UAP1 participates in the synthesis of UDP-GlcNAc an essential precursor for glycoproteins and glycosaminoglycans. This function positions UAP1 as a component of a larger amino sugar complex involved in glycosylation which is critical for protein folding and stability. By producing UDP-GlcNAc UAP1 influences various cellular functions including signaling and metabolism.

Pathways

UAP1 lies at the intersection of the hexosamine biosynthetic pathway and glycosylation processes. It provides the substrate necessary for O-GlcNAcylation a modification that impacts protein function and regulation. This pathway links UAP1 to other proteins such as O-GlcNAc transferase (OGT) which modifies serine and threonine residues of many proteins. Together they affect cellular activities like nutrient sensing and transcription.

Altered UAP1 function links to metabolic and degenerative conditions. Abnormal UAP1 activity associates with type 2 diabetes due to its role in the hexosamine biosynthetic pathway that modulates insulin signaling. Furthermore its involvement in glycosylation pathways connects UAP1 to congenital disorders of glycosylation (CDG) where deficient glycosylation leads to multiorgan dysfunction. Changes in UAP1 expression or function can impair glycosylation potentially leading to dysregulation of related proteins such as insulin receptor substrates in diabetes or enzymes impacted in CDG.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the last step in biosynthesis of uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by converting UTP and glucosamine 1-phosphate (GlcNAc-1-P) to the sugar nucleotide (PubMed : 9603950, PubMed : 9765219). Also converts UTP and galactosamine 1-phosphate (GalNAc-1-P) into uridine diphosphate-N-acetylgalactosamine (UDP-GalNAc) (PubMed : 9765219). In addition to its role in metabolism, acts as a regulator of innate immunity in response to virus infection by mediating pyrophosphorylation of IRF3 : catalyzes pyrophosphorylation of IRF3 phosphorylated at 'Ser-386' by TBK1, promoting IRF3 dimerization and activation, leading to type I interferon responses (PubMed : 36603579).. Isoform AGX1. Isoform AGX1 has 2 to 3 times higher activity towards galactosamine 1-phosphate (GalNAc-1-P).. Isoform AGX1. Isoform AGX2 has 8 times more activity towards glucosamine 1-phosphate (GlcNAc-1-P).
See full target information UAP1
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Product promise

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For full details, please see our Terms & Conditions

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