AB247984

Anti-UBA3 antibody [MILR42872] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal UBA3 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

UBE1C, UBA3, NEDD8-activating enzyme E1 catalytic subunit, NEDD8-activating enzyme E1C, Ubiquitin-activating enzyme E1C, Ubiquitin-like modifier-activating enzyme 3, Ubiquitin-activating enzyme 3

1 Images

Unknown

Western blot - Anti-UBA3 antibody [MILR42872] - BSA and Azide free (AB247984)

This data was developed using ab124728, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-UBA3 antibody [MILR42872] (<a href='/en-us/products/primary-antibodies/uba3-antibody-milr42872-ab124728'>ab124728</a>)

Lane 1:

Fetal lung cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

RAW 264.7 cell lysate at 10 µg

Lane 5:

HepG2 cell lysate at 10 µg

Lane 6:

K562 cell lysate at 10 µg

Predicted band size: 52 kDa

false

Unconjugated

Anti-UBA3 antibody [MILR42872]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

MILR42872

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab247984 is the carrier-free version of ab124728.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target UBA3 also known as ubiquitin-like modifier-activating enzyme 3 functions mechanically as an essential enzyme in the NEDD8-conjugation pathway. It weighs approximately 50 kDa. UBA3 expresses itself in various tissues but shows widespread expression across different cell types. It operates as a catalytic subunit of the E1 enzyme complex that initiates the activation and transfer of the NEDD8 protein. This process is important for the subsequent conjugation to target proteins.

Biological function summary

The process involving UBA3 relates to protein modification primarily through neddylation. UBA3 couples with the protein NAE1 to form the NEDD8 activating enzyme complex. This enzyme complex activates NEDD8 a ubiquitin-like protein that modifies cullin-based E3 ligases influencing their function. By modifying these E3 ligases UBA3 controls various cellular processes including cell cycle progression and signal transduction ensuring proper cell function and adaptation to environmental cues.

Pathways

UBA3 is integral to the NEDD8 conjugation pathway influencing cell cycle and transcriptional regulation. It interacts with proteins such as cullin 1 and cullin 3 within this pathway. By activating NEDD8 UBA3 facilitates cullin-RING ubiquitin ligase complexes which play significant roles in the ubiquitin-proteasome pathway. This interaction controls protein degradation a vital process for maintaining protein homeostasis and preventing cellular dysfunction.

UBA3 involvement in neddylation links it to cancer and neurodegenerative diseases. Aberrant UBA3 activity relates to cancers as dysregulation of the NEDD8 pathway can lead to uncontrolled cell proliferation. In neurodegenerative disorders such as Alzheimer's disease altered neddylation can disrupt normal protein homeostasis contributing to neuron damage. UBA3's interaction with protein partners like cullins connects it to various pathological states highlighting its importance in disease development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.

See full target information UBA3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinics (Sao Paulo, Brazil) 77:100019 PubMed35397366

2022

IRF4 suppresses osteogenic differentiation of BM-MSCs by transcriptionally activating miR-636/DOCK9 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xuepu Zhang,Yue Zhang,Limin Yang,Yuexin Wu,Xiaohu Ma,Gang Tong,Zhaoliang Ban,Haosen Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UBA3 antibody [MILR42872] - BSA and Azide free

Western blot - Anti-UBA3 antibody [MILR42872] - BSA and Azide free (AB247984)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise