AB177507

Anti-UBA5 antibody

Name: Anti-UBA5 antibody (ab177507) | Abcam
Brand: Abcam Limited
SKU: AB177507

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(3 Publications)

Rabbit Polyclonal UBA5 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human UBA5 aa 300-400.

View Alternative Names

UBE1DC1, UBA5, Ubiquitin-like modifier-activating enzyme 5, Ubiquitin-activating enzyme 5, ThiFP1, UFM1-activating enzyme, Ubiquitin-activating enzyme E1 domain-containing protein 1

2 Images

Supplier Data

Immunoprecipitation - Anti-UBA5 antibody (AB177507)

Detection of UBA5 in Immunoprecipitates of Jurkat whole cell lysates (1 mg for IP, 20% of IP loaded) using ab177507 at 6 μg/mg lysate for IP (Lane 1). For WB detection ab177507 was used at 1 μg/ml. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-UBA5 antibody (ab177507)

Predicted band size: 45 kDa

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Supplier Data

Western blot - Anti-UBA5 antibody (AB177507)

All lanes:

Western blot - Anti-UBA5 antibody (ab177507) at 0.4 µg/mL

Lane 1:

Jurkat whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Predicted band size: 45 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human UBA5 aa 300-400. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab177507 was affinity purified using an epitope specific to UBA5 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBA5 also known as Ubiquitin-like modifier-activating enzyme 5 is an E1 enzyme that plays a mechanical role in activating ubiquitin-fold modifier 1 (UFM1) for protein modification. It has a molecular mass of about 44 kDa and performs its function in the nucleus and cytoplasm within human cells. The expression of UBA5 is not limited to specific tissues; instead it shows widespread expression across many tissues supporting an important role in various cellular processes.

Biological function summary

UBA5 is implicated in the UFM1 conjugation system. This process modifies specific proteins and affects protein stability interaction and degradation. UBA5 operates in complex with UFC1 an E2 enzyme enabling the transfer of UFM1 to target proteins. Its activity is essential for maintaining cellular homeostasis particularly in the endoplasmic reticulum stress response through protein modification and regulation tasks.

Pathways

The UBA5-UFM1 axis integrates into the cellular stress response pathway and the endoplasmic reticulum-associated degradation (ERAD) pathway. Through these pathways UBA5 collaborates with proteins like UFL1 further connecting these pathways by tagging proteins with UFM1 to mark them for degradation or functional modification. The coordination between UBA5 and UFL1 supports efficient protein quality control and maintains cellular equilibrium under stress conditions.

The dysfunction or altered expression of UBA5 associates with neurodevelopmental disorders and some cancers. Changes in UBA5 activity can lead to imbalances in protein homeostasis contributing to pathogenic mechanisms in neurodevelopmental disorders where other proteins like EIF4A1 also play a role. In cancer alterations in UBA5 expression may influence tumor progression as seen in cases where UBA5 levels affect pathways that involve p53 an important tumor suppressor protein.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E1-like enzyme which specifically catalyzes the first step in ufmylation (PubMed : 15071506, PubMed : 18442052, PubMed : 20368332, PubMed : 25219498, PubMed : 26929408, PubMed : 27545674, PubMed : 27545681, PubMed : 27653677, PubMed : 30412706, PubMed : 30626644, PubMed : 34588452). Activates UFM1 by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a UFM1-E1 thioester and free AMP (PubMed : 20368332, PubMed : 26929408, PubMed : 27653677, PubMed : 30412706). Activates UFM1 via a trans-binding mechanism, in which UFM1 interacts with distinct sites in both subunits of the UBA5 homodimer (PubMed : 27653677). Trans-binding also promotes stabilization of the UBA5 homodimer, and enhances ATP-binding (PubMed : 29295865). Transfer of UFM1 from UBA5 to the E2-like enzyme UFC1 also takes place using a trans mechanism (PubMed : 27653677, PubMed : 34588452). Ufmylation plays a key role in various processes, such as ribosome recycling, response to DNA damage, interferon response or reticulophagy (also called ER-phagy) (PubMed : 30412706, PubMed : 32160526, PubMed : 35394863). Ufmylation is essential for erythroid differentiation of both megakaryocytes and erythrocytes (By similarity).

See full target information UBA5

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Brain communications 5:fcad317 PubMed38046095

2023

Genetic model of UBA5 deficiency highlights the involvement of both peripheral and central nervous systems and identifies widespread mitochondrial abnormalities.

Applications

Unspecified application

Species

Unspecified reactive species

Rita J Serrano,Viola Oorschot,Dashika Palipana,Vanessa Calcinotto,Carmen Sonntag,Georg Ramm,Robert J Bryson-Richardson

Molecules (Basel, Switzerland) 26: PubMed33430125

2021

The UFM1 Pathway Impacts HCMV US2-Mediated Degradation of HLA Class I.

Applications

Unspecified application

Species

Unspecified reactive species

A B C Schuren,I G J Boer,E M Bouma,M L Van de Weijer,A I Costa,P Hubel,A Pichlmair,R J Lebbink,E J H J Wiertz

Reproductive sciences (Thousand Oaks, Calif.) 28:1476-1488 PubMed33398850

2021

Uteroplacental Insufficiency with Hypoxia Upregulates Placental PPARγ-KMT5A Axis in the Rat.

Applications

Unspecified application

Species

Unspecified reactive species

Emily Barrett,Amy Loverin,Haimei Wang,Michelle Carlson,Tricia D Larsen,Mariana M Almeida,Jenna Whitman,Michelle L Baack,Lisa A Joss-Moore

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UBA5 antibody

Immunoprecipitation - Anti-UBA5 antibody (AB177507)

Western blot - Anti-UBA5 antibody (AB177507)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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