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AB228918

Anti-Uba6 antibody

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(2 Publications)

Rabbit Polyclonal UBA6 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human UBA6.

View Alternative Names

MOP4, UBE1L2, UBA6, Ubiquitin-like modifier-activating enzyme 6, Ubiquitin-activating enzyme 6, Monocyte protein 4, Ubiquitin-activating enzyme E1-like protein 2, MOP-4, E1-L2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uba6 antibody (AB228918)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uba6 antibody (AB228918)

Paraffin-embedded human colon carcinoma tissue stained for Uba6 using ab228918 at 1/250 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uba6 antibody (AB228918)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uba6 antibody (AB228918)

Paraffin-embedded human colon carcinoma tissue stained for Uba6 using ab228918 at 1/250 dilution in immunohistochemical analysis.

Western blot - Anti-Uba6 antibody (AB228918)
  • WB

Supplier Data

Western blot - Anti-Uba6 antibody (AB228918)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Uba6 antibody (ab228918) at 1/2000 dilution

All lanes:

IMR32 whole cell lysate at 30 µg

Predicted band size: 118 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human UBA6. The exact immunogen used to generate this antibody is proprietary information.

A0AVT1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Uba6 also known as ubiquitin-like modifier activating enzyme 6 is an E1-like ubiquitin-activating enzyme. It has an approximate mass of around 120 kDa. This enzyme facilitates the activation of ubiquitin and similar proteins by catalyzing the formation of a ubiquitin-adenylate intermediate. Uba6 is expressed in a wide range of human tissues with higher levels observed in the brain heart and skeletal muscle. Its expression suggests a need for its activity in diverse biological processes across different tissue types.
Biological function summary

Uba6 modifies proteins by attaching ubiquitin moieties which tags them for various fates including degradation or location changes. Uba6 functions as a part of the ubiquitin-proteasome system (UPS) a critical protein degradation and quality control pathway. In this system Uba6 collaborates with E2 conjugating enzymes such as Ube2z which transfer ubiquitin from Uba6 to substrate proteins. This involvement emphasizes its role in protein homeostasis and cellular stress responses by controlling protein stability.

Pathways

Uba6 takes part in the maintenance of protein homeostasis and also influences the NEDD8 modification pathway. The UPS pathway integrates Uba6 with processes involving protein turnover and stress responses maintaining cellular oxidative stress levels. In the NEDD8 pathway Uba6 interacts with proteins like UBE2D which influence protein degradation scenarios that differ from those managed by the classic ubiquitin system. These pathways highlight the diverse regulatory roles Uba6 holds in managing intracellular protein dynamics.

Uba6 plays a role in neurodegenerative diseases and some cancers. In Alzheimer's disease Uba6 affects the accumulation of misfolded proteins connecting its action to the degradation pathways that include related ubiquitination enzymes. It also shows relevance to tumor progression in cancers where aberrations in ubiquitin-mediated pathways play a part in unregulated cell growth. The functions of Uba6 in these conditions link it with disorders of protein homeostasis where proteins like UBE2Z are involved.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility.
See full target information UBA6
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:758 PubMed39424627

2024

The interaction between UBR7 and PRMT5 drives PDAC resistance to gemcitabine by regulating glycolysis and immune microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Maoxiao Feng,Qinlian Jiao,Yidan Ren,Xiaoyan Liu,Zihan Gao,Zhengjun Li,Yunshan Wang,Miaoqing Zhao,Lei Bi

International journal of molecular sciences 25: PubMed38474091

2024

UBA6 Inhibition Accelerates Lysosomal TRPML1 Depletion and Exosomal Secretion in Lung Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dongun Lee,Peter Chang-Whan Lee,Jeong Hee Hong
View all publications
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