AB197083

Anti-UBAP2 antibody

Name: Anti-UBAP2 antibody (ab197083) | Abcam
Brand: Abcam Limited
SKU: AB197083
Price: 520 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal UBAP2 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human UBAP2 aa 450-500.

View Alternative Names

KIAA1491, UBAP2, Ubiquitin-associated protein 2, UBAP-2, RNA polymerase II degradation factor UBAP2

2 Images

Supplier Data

Immunoprecipitation - Anti-UBAP2 antibody (AB197083)

Detection of UBAP2 in immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab197083 at 6 μg/mg lysate for IP (Lane

and at 1 μg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-UBAP2 antibody (ab197083)

Predicted band size: 117 kDa

false

Supplier Data

Western blot - Anti-UBAP2 antibody (AB197083)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-UBAP2 antibody (ab197083) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 117 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human UBAP2 aa 450-500. The exact immunogen used to generate this antibody is proprietary information.

Q5T6F2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab197083 was affinity purified using an epitope specific to UBAP2 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBAP2 also known as Ubiquitin-Associated Protein 2 plays a role in cellular processes by interacting with ubiquitin-related pathways. It has a mass of approximately 115 kDa. This protein functions mainly in the cytoplasm but is also present in the nucleus in certain cell types. UBAP2 expression occurs in a wide range of tissues with higher levels observed in immune cells such as lymphocytes.

Biological function summary

UBAP2 interacts with components of the endosomal sorting complex required for transport (ESCRT) machinery. This interaction suggests a role in vesicle trafficking and membrane dynamics. UBAP2 has not been reported as part of a complex but might work closely with other ubiquitin-interacting proteins to regulate endocytic processes. It may assist in sorting endocytosed proteins for degradation or recycling.

Pathways

UBAP2 takes part in ubiquitin-mediated processes and is linked to the intracellular trafficking pathway. Its interaction with the ESCRT machinery indicates participation in endosomal and lysosomal pathways. Related proteins include ubiquitin and TSG101 which are involved in coordinating the sorting and transport of proteins through the endosomal system.

Researchers investigate UBAP2 for its potential link to neurodegenerative diseases such as Alzheimer's disease and certain cancers. Studies propose that altered ubiquitin pathways may contribute to these conditions implicating proteins like TSG101. Alterations in UBAP2 function might therefore disrupt normal cellular processes contributing to disease progression and pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Recruits the ubiquitination machinery to RNA polymerase II for polyubiquitination, removal and degradation, when the transcription-coupled nucleotide excision repair (TC-NER) machinery fails to resolve DNA damage (PubMed : 35633597). May promote the degradation of ANXA2 (PubMed : 27121050).

See full target information UBAP2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:110893 PubMed39328932

2024

DDI2 protease controls embryonic development and inflammation via TCF11/NRF1.

Applications

Unspecified application

Species

Unspecified reactive species

Monika Nedomova,Stefanie Haberecht-Müller,Sophie Möller,Simone Venz,Michaela Prochazkova,Jan Prochazka,Frantisek Sedlak,Kallayanee Chawengsaksophak,Elke Hammer,Petr Kasparek,Michael Adamek,Radislav Sedlacek,Jan Konvalinka,Elke Krüger,Klara Grantz Saskova

Nature communications 14:3668 PubMed37339951

2023

UBAP2 plays a role in bone homeostasis through the regulation of osteoblastogenesis and osteoclastogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jeonghyun Kim,Bo-Young Kim,Jeong-Soo Lee,Yun-Mi Jeong,Hyun-Ju Cho,Eunkuk Park,Dowan Kim,Sung-Soo Kim,Bom-Taeck Kim,Yong Jun Choi,Ye-Yeon Won,Hyun-Seok Jin,Yoon-Sok Chung,Seon-Yong Jeong

Cell 176:831-843.e22 PubMed30735634

2019

Widespread and Functional RNA Circularization in Localized Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sujun Chen,Vincent Huang,Xin Xu,Julie Livingstone,Fraser Soares,Jouhyun Jeon,Yong Zeng,Junjie Tony Hua,Jessica Petricca,Haiyang Guo,Miranda Wang,Fouad Yousif,Yuzhe Zhang,Nilgun Donmez,Musaddeque Ahmed,Stas Volik,Anna Lapuk,Melvin L K Chua,Lawrence E Heisler,Adrien Foucal,Natalie S Fox,Michael Fraser,Vinayak Bhandari,Yu-Jia Shiah,Jiansheng Guan,Jixi Li,Michèle Orain,Valérie Picard,Hélène Hovington,Alain Bergeron,Louis Lacombe,Yves Fradet,Bernard Têtu,Stanley Liu,Felix Feng,Xue Wu,Yang W Shao,Malgorzata A Komor,Cenk Sahinalp,Colin Collins,Youri Hoogstrate,Mark de Jong,Remond J A Fijneman,Teng Fei,Guido Jenster,Theodorus van der Kwast,Robert G Bristow,Paul C Boutros,Housheng Hansen He

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UBAP2 antibody

Immunoprecipitation - Anti-UBAP2 antibody (AB197083)

Western blot - Anti-UBAP2 antibody (AB197083)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

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Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise