Rabbit Polyclonal UBAP2 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human UBAP2 aa 450-500.
Images
Publications
Filter by
- Nature communications 14:36682023UBAP2 plays a role in bone homeostasis through the regulation of osteoblastogenesis and osteoclastogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeonghyun Kim,Bo-Young Kim,Jeong-Soo Lee,Yun-Mi Jeong,Hyun-Ju Cho,Eunkuk Park,Dowan Kim,Sung-Soo Kim,Bom-Taeck Kim,Yong Jun Choi,Ye-Yeon Won,Hyun-Seok Jin,Yoon-Sok Chung,Seon-Yong JeongPubMed 37339951
- Cell 176:831-843.e222019Widespread and Functional RNA Circularization in Localized Prostate Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSujun Chen,Vincent Huang,Xin Xu,Julie Livingstone,Fraser Soares,Jouhyun Jeon,Yong Zeng,Junjie Tony Hua,Jessica Petricca,Haiyang Guo,Miranda Wang,Fouad Yousif,Yuzhe Zhang,Nilgun Donmez,Musaddeque Ahmed,Stas Volik,Anna Lapuk,Melvin L K Chua,Lawrence E Heisler,Adrien Foucal,Natalie S Fox,Michael Fraser,Vinayak Bhandari,Yu-Jia Shiah,Jiansheng Guan,Jixi Li,Michèle Orain,Valérie Picard,Hélène Hovington,Alain Bergeron,Louis Lacombe,Yves Fradet,Bernard Têtu,Stanley Liu,Felix Feng,Xue Wu,Yang W Shao,Malgorzata A Komor,Cenk Sahinalp,Colin Collins,Youri Hoogstrate,Mark de Jong,Remond J A Fijneman,Teng Fei,Guido Jenster,Theodorus van der Kwast,Robert G Bristow,Paul C Boutros,Housheng Hansen HePubMed 30735634
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human UBAP2 aa 450-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q5T6F2
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Cynomolgus monkey | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cynomolgus monkey, Rhesus monkey, Gorilla | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cynomolgus monkey, Rhesus monkey, Gorilla | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Recruits the ubiquitination machinery to RNA polymerase II for polyubiquitination, removal and degradation, when the transcription-coupled nucleotide excision repair (TC-NER) machinery fails to resolve DNA damage (PubMed:35633597). May promote the degradation of ANXA2 (PubMed:27121050).
Alternative names
KIAA1491, UBAP2, Ubiquitin-associated protein 2, UBAP-2, RNA polymerase II degradation factor UBAP2
Recommended products
Rabbit Polyclonal UBAP2 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human UBAP2 aa 450-500.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human UBAP2 aa 450-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q5T6F2
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab197083 was affinity purified using an epitope specific to UBAP2 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UBAP2 also known as Ubiquitin-Associated Protein 2 plays a role in cellular processes by interacting with ubiquitin-related pathways. It has a mass of approximately 115 kDa. This protein functions mainly in the cytoplasm but is also present in the nucleus in certain cell types. UBAP2 expression occurs in a wide range of tissues with higher levels observed in immune cells such as lymphocytes.
- Biological function summary
UBAP2 interacts with components of the endosomal sorting complex required for transport (ESCRT) machinery. This interaction suggests a role in vesicle trafficking and membrane dynamics. UBAP2 has not been reported as part of a complex but might work closely with other ubiquitin-interacting proteins to regulate endocytic processes. It may assist in sorting endocytosed proteins for degradation or recycling.
- Pathways
UBAP2 takes part in ubiquitin-mediated processes and is linked to the intracellular trafficking pathway. Its interaction with the ESCRT machinery indicates participation in endosomal and lysosomal pathways. Related proteins include ubiquitin and TSG101 which are involved in coordinating the sorting and transport of proteins through the endosomal system.
- Associated diseases and disorders
Researchers investigate UBAP2 for its potential link to neurodegenerative diseases such as Alzheimer's disease and certain cancers. Studies propose that altered ubiquitin pathways may contribute to these conditions implicating proteins like TSG101. Alterations in UBAP2 function might therefore disrupt normal cellular processes contributing to disease progression and pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-UBAP2 antibody (ab197083)
Lysates prepared using NETN lysis buffer.
All lanes: Western blot - Anti-UBAP2 antibody (ab197083) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: 293T whole cell lysate at 50 µg
Lane 3: Jurkat whole cell lysate at 50 µg
Lane 4: TCMK-1 whole cell lysate at 50 µg
Lane 5: NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 117 kDa
Exposure time: 30s
Immunoprecipitation - Anti-UBAP2 antibody (ab197083)
Detection of UBAP2 in immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab197083 at 6 μg/mg lysate for IP (Lane 1) and at 1 μg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds.
All lanes: Immunoprecipitation - Anti-UBAP2 antibody (ab197083)
Predicted band size: 117 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com