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AB246989

Anti-UBAP2L antibody

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Rabbit Polyclonal UBAP2L antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human UBAP2L aa 1000-1100.

View Alternative Names

KIAA0144, NICE4, UBAP2L, Ubiquitin-associated protein 2-like, Protein NICE-4, RNA polymerase II degradation factor UBAP2L

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBAP2L antibody (AB246989)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBAP2L antibody (AB246989)

Paraffin-embedded human cerebral cortex tissue stained for UBAP2L using ab246989 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-UBAP2L antibody (AB246989)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-UBAP2L antibody (AB246989)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for UBAP2L (green) using ab246989 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human UBAP2L aa 1000-1100. The exact immunogen used to generate this antibody is proprietary information.

Q14157

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBAP2L also known as USP10-associated protein 2-like has a molecular mass of approximately 115 kDa. Mechanically it functions as a translational regulator. This protein is enriched in cellular compartments including the cytoplasm and stress granules. It interacts with multiple proteins and is found in many tissues suggesting a role in cellular stress responses and phase separation events.
Biological function summary

UBAP2L influences stress granule dynamics and protein synthesis under stress conditions. It is important for assembly of these translationally silent granules suggesting its involvement in cell survival. As a part of a larger protein interaction complex UBAP2L contributes to cellular homeostasis. Its interaction partners include G3BP1 which serves as an important player in stress granule nucleation.

Pathways

UBAP2L integrates into the mRNA surveillance pathway and the cellular stress response pathway. It interacts with proteins like USP10 and G3BP1 to facilitate stress granule formation and disassembly critical for proper mRNA metabolism. Through these pathways UBAP2L helps maintain proteostasis under cellular stress affecting translation and decay of mRNA.

UBAP2L has implications in neurodegenerative diseases and cancer. Aberrations in its function contribute to Alzheimer's disease through dysregulated stress granule assembly involving proteins like TDP-43. Additionally it plays a role in tumor progression where alterations in its expression correlate with poor prognosis interacting with oncogenic proteins such as HNRNPA1 linked to metastasis and cellular proliferation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Recruits the ubiquitination machinery to RNA polymerase II for polyubiquitination, removal and degradation, when the transcription-coupled nucleotide excision repair (TC-NER) machinery fails to resolve DNA damage (PubMed : 35633597). Plays an important role in the activity of long-term repopulating hematopoietic stem cells (LT-HSCs) (By similarity). Is a regulator of stress granule assembly, required for their efficient formation (PubMed : 29395067, PubMed : 35977029). Required for proper brain development and neocortex lamination (By similarity).
See full target information UBAP2L
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Product promise

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