Rabbit Polyclonal UBAP2L antibody. Suitable for IP, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human UBAP2L.
View Alternative Names
KIAA0144, NICE4, UBAP2L, Ubiquitin-associated protein 2-like, Protein NICE-4, RNA polymerase II degradation factor UBAP2L
- IP
Supplier Data
Immunoprecipitation - Anti-UBAP2L antibody (AB70319)
UBAP2L was immunoprecipitated from HEK-293T whole cell lysate (1 mg per IP reaction, 20% loaded) with ab70319 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab70319 at 0.04 µg/mL.
Lane 1 : ab70319 IP in HEK-293T whole cell lysate.
Lane 2 : ab70319 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG in HEK-293T whole cell lysate.
Detection : Chemiluminescence with an exposure time of 1 second.
All lanes:
Immunoprecipitation - Anti-UBAP2L antibody (ab70319)
Predicted band size: 115 kDa
false
- WB
Supplier Data
Western blot - Anti-UBAP2L antibody (AB70319)
All lanes:
Western blot - Anti-UBAP2L antibody (ab70319) at 0.04 µg/mL
Lane 1:
HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg
Lane 2:
HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
Lane 3:
Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg
Lane 4:
K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 10 µg
Lane 5:
MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 10 µg
Predicted band size: 115 kDa
false
Exposure time: 10s
Reactivity data
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
UBAP2L influences stress granule dynamics and protein synthesis under stress conditions. It is important for assembly of these translationally silent granules suggesting its involvement in cell survival. As a part of a larger protein interaction complex UBAP2L contributes to cellular homeostasis. Its interaction partners include G3BP1 which serves as an important player in stress granule nucleation.
Pathways
UBAP2L integrates into the mRNA surveillance pathway and the cellular stress response pathway. It interacts with proteins like USP10 and G3BP1 to facilitate stress granule formation and disassembly critical for proper mRNA metabolism. Through these pathways UBAP2L helps maintain proteostasis under cellular stress affecting translation and decay of mRNA.
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Target data
Publications (8)
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Science advances 8:eabo7112 PubMed35977029
2022
Applications
Unspecified application
Species
Unspecified reactive species
Molecular cancer 21:92 PubMed35366893
2022
Applications
Unspecified application
Species
Unspecified reactive species
Life sciences 279:119645 PubMed34043991
2021
Applications
Unspecified application
Species
Unspecified reactive species
Experimental and therapeutic medicine 20:2252-2261 PubMed32765702
2020
Applications
Unspecified application
Species
Unspecified reactive species
Current medical science 38:1081-1089 PubMed30536073
2018
Applications
Unspecified application
Species
Unspecified reactive species
Breast cancer (Tokyo, Japan) 25:224-232 PubMed29196913
2017
Applications
WB
Species
Human
International journal of molecular medicine 36:1012-8 PubMed26310274
2015
Applications
WB
Species
Unspecified reactive species
Oncology reports 32:1578-84 PubMed25069639
2014
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
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