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AB70319

Anti-UBAP2L antibody

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(8 Publications)

Rabbit Polyclonal UBAP2L antibody. Suitable for IP, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human UBAP2L.

View Alternative Names

KIAA0144, NICE4, UBAP2L, Ubiquitin-associated protein 2-like, Protein NICE-4, RNA polymerase II degradation factor UBAP2L

2 Images
Immunoprecipitation - Anti-UBAP2L antibody (AB70319)
  • IP

Supplier Data

Immunoprecipitation - Anti-UBAP2L antibody (AB70319)

UBAP2L was immunoprecipitated from HEK-293T whole cell lysate (1 mg per IP reaction, 20% loaded) with ab70319 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab70319 at 0.04 µg/mL.
Lane 1 : ab70319 IP in HEK-293T whole cell lysate.
Lane 2 : ab70319 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG in HEK-293T whole cell lysate.
Detection : Chemiluminescence with an exposure time of 1 second.

All lanes:

Immunoprecipitation - Anti-UBAP2L antibody (ab70319)

Predicted band size: 115 kDa

false

Western blot - Anti-UBAP2L antibody (AB70319)
  • WB

Supplier Data

Western blot - Anti-UBAP2L antibody (AB70319)

All lanes:

Western blot - Anti-UBAP2L antibody (ab70319) at 0.04 µg/mL

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg

Lane 4:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 10 µg

Lane 5:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Predicted band size: 115 kDa

false

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human UBAP2L. The exact immunogen used to generate this antibody is proprietary information.

Q14157

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBAP2L also known as USP10-associated protein 2-like has a molecular mass of approximately 115 kDa. Mechanically it functions as a translational regulator. This protein is enriched in cellular compartments including the cytoplasm and stress granules. It interacts with multiple proteins and is found in many tissues suggesting a role in cellular stress responses and phase separation events.
Biological function summary

UBAP2L influences stress granule dynamics and protein synthesis under stress conditions. It is important for assembly of these translationally silent granules suggesting its involvement in cell survival. As a part of a larger protein interaction complex UBAP2L contributes to cellular homeostasis. Its interaction partners include G3BP1 which serves as an important player in stress granule nucleation.

Pathways

UBAP2L integrates into the mRNA surveillance pathway and the cellular stress response pathway. It interacts with proteins like USP10 and G3BP1 to facilitate stress granule formation and disassembly critical for proper mRNA metabolism. Through these pathways UBAP2L helps maintain proteostasis under cellular stress affecting translation and decay of mRNA.

UBAP2L has implications in neurodegenerative diseases and cancer. Aberrations in its function contribute to Alzheimer's disease through dysregulated stress granule assembly involving proteins like TDP-43. Additionally it plays a role in tumor progression where alterations in its expression correlate with poor prognosis interacting with oncogenic proteins such as HNRNPA1 linked to metastasis and cellular proliferation.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Recruits the ubiquitination machinery to RNA polymerase II for polyubiquitination, removal and degradation, when the transcription-coupled nucleotide excision repair (TC-NER) machinery fails to resolve DNA damage (PubMed : 35633597). Plays an important role in the activity of long-term repopulating hematopoietic stem cells (LT-HSCs) (By similarity). Is a regulator of stress granule assembly, required for their efficient formation (PubMed : 29395067, PubMed : 35977029). Required for proper brain development and neocortex lamination (By similarity).
See full target information UBAP2L

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Science advances 8:eabo7112 PubMed35977029

2022

De novo variants in genes regulating stress granule assembly associate with neurodevelopmental disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangbin Jia,Shujie Zhang,Senwei Tan,Bing Du,Mei He,Haisong Qin,Jia Chen,Xinyu Duan,Jingsi Luo,Fei Chen,Luping Ouyang,Jian Wang,Guodong Chen,Bin Yu,Ge Zhang,Zimin Zhang,Yongqing Lyu,Yi Huang,Jian Jiao,Jin Yun Helen Chen,Kathryn J Swoboda,Emanuele Agolini,Antonio Novelli,Chiara Leoni,Giuseppe Zampino,Gerarda Cappuccio,Nicola Brunetti-Pierri,Benedicte Gerard,Emmanuelle Ginglinger,Julie Richer,Hugh McMillan,Alexandre White-Brown,Kendra Hoekzema,Raphael A Bernier,Evangeline C Kurtz-Nelson,Rachel K Earl,Claartje Meddens,Marielle Alders,Meredith Fuchs,Roseline Caumes,Perrine Brunelle,Thomas Smol,Ryan Kuehl,Debra-Lynn Day-Salvatore,Kristin G Monaghan,Michelle M Morrow,Evan E Eichler,Zhengmao Hu,Ling Yuan,Jieqiong Tan,Kun Xia,Yiping Shen,Hui Guo

Molecular cancer 21:92 PubMed35366893

2022

Circular RNA MTCL1 promotes advanced laryngeal squamous cell carcinoma progression by inhibiting C1QBP ubiquitin degradation and mediating beta-catenin activation.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Wang,Anqi Sun,Aihui Yan,Jian Yao,Haibo Huang,Ziming Gao,Tao Han,Jia Gu,Ni Li,Huizhe Wu,Kai Li

Life sciences 279:119645 PubMed34043991

2021

Norcantharidin-blocked ANXA2P2 inhibits fibroblast proliferation by increasing UBAP2L mRNA stability through LIN28B.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Huang,Qixia Yang,Tiantian Wang

Experimental and therapeutic medicine 20:2252-2261 PubMed32765702

2020

MicroRNA-19a-3p inhibits the cellular proliferation and invasion of non-small cell lung cancer by downregulating UBAP2L.

Applications

Unspecified application

Species

Unspecified reactive species

Yuejiang Pan,Ke Jin,Xuan Xie,Kexi Wang,Huizhong Zhang

Current medical science 38:1081-1089 PubMed30536073

2018

Upregulation of UBAP2L in Bone Marrow Mesenchymal Stem Cells Promotes Functional Recovery in Rats with Spinal Cord Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Guan-Lin Lin,Huan Wang,Jun Dai,Xiao Li,Ming Guan,Qing Ding,Huai-Xi Wang,Huang Fang

Breast cancer (Tokyo, Japan) 25:224-232 PubMed29196913

2017

UBAP2L silencing inhibits cell proliferation and G2/M phase transition in breast cancer.

Applications

WB

Species

Human

Jing He,Yuanping Chen,Lu Cai,Zelei Li,Xiaoqing Guo

International journal of molecular medicine 36:1012-8 PubMed26310274

2015

Downregulation of ubiquitin-associated protein 2-like with a short hairpin RNA inhibits human glioma cell growth in vitro.

Applications

WB

Species

Unspecified reactive species

Bing Zhao,Gang Zong,Yongsheng Xie,Jia Li,Hongliang Wang,Erbao Bian

Oncology reports 32:1578-84 PubMed25069639

2014

Knockdown of ubiquitin associated protein 2-like inhibits the growth and migration of prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Li,Yiran Huang
View all publications

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