JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB197168

Anti-UBASH3A antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal UBASH3A antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human UBASH3A aa 100 to C-terminus.

View Alternative Names

STS2, UBASH3A, Ubiquitin-associated and SH3 domain-containing protein A, Cbl-interacting protein 4, Suppressor of T-cell receptor signaling 2, T-cell ubiquitin ligand 1, CLIP4, STS-2, TULA-1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBASH3A antibody (AB197168)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBASH3A antibody (AB197168)

Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human fetal spleen tissue labeling UBASH3A with ab197168 at 1/100 dilution.

Western blot - Anti-UBASH3A antibody (AB197168)
  • WB

Supplier Data

Western blot - Anti-UBASH3A antibody (AB197168)

All lanes:

Western blot - Anti-UBASH3A antibody (ab197168) at 1/500 dilution

All lanes:

Human fetal heart lysate

Predicted band size: 74 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human UBASH3A aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P57075

style
left-column

Complementary Products

Primary Antibodies

AB251234

Anti-Sts1 antibody [EPR16980] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Sts1 antibody [EPR16980] - BSA and Azide free (AB251234)

  • 0
  • 0
Primary Antibodies

AB239081

Anti-DGKZ/DGK-zeta antibody [EPR22040-80]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-DGKZ/DGK-zeta antibody [EPR22040-80] (AB239081)

  • 0
  • 0
Primary Antibodies

AB315022

Anti-CBLB antibody [EPR28082-32] - BSA and Azide free

RabMAb|KO Validated|Recombinant

Western blot - Anti-CBLB antibody [EPR28082-32] - BSA and Azide free (AB315022)

  • 0
  • 0
Primary Antibodies

AB229829

Anti-NFS1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFS1 antibody (AB229829)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 200µL
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBASH3A also known as suppressor of T-cell receptor signaling 1 (STS-1) is a protein involved in regulating T-cell signaling. It has a molecular mass of approximately 67 kDa. UBASH3A is expressed mainly in lymphoid tissues including the thymus and spleen. The protein contains a Src homology 3 (SH3) domain and a ubiquitin-associated (UBA) domain which are important for its interactions with other proteins and its function in cellular signaling processes.
Biological function summary

UBASH3A plays an important role in modulating immune responses. It regulates T-cell receptor (TCR) signaling by acting as a negative regulator ensuring proper immune tolerance and preventing overstimulation of immune responses. UBASH3A is not known to be part of a larger protein complex. It interacts with signaling molecules and adaptor proteins to modulate signal transduction pathways in T-cells controlling their activation and proliferation.

Pathways

UBASH3A influences T-cell signaling pathways and the broader immune response. It is involved in the TCR signaling pathway which is essential for the activation and development of T-cells. Through this pathway UBASH3A interacts with proteins like LCK and ZAP-70 which are necessary for TCR-induced signaling events leading to cytokine production and cell-mediated immune responses. Additionally UBASH3A is connected to pathways controlling apoptosis where it fine-tunes the balance between cell survival and programmed cell death.

UBASH3A has implications in autoimmune diseases and cancer. Its role as a negative regulator of TCR signaling links it to autoimmune conditions such as type 1 diabetes where improper TCR signaling can lead to autoimmune attack of pancreatic beta cells. Furthermore altered expression or function of UBASH3A can impact cancer progression particularly in hematological cancers like leukemia where T-cell signaling dysregulation can promote proliferation. Proteins such as CTLA-4 and PD-1 which are involved in immune checkpoint pathways can be indirectly influenced by UBASH3A activity affecting disease progression and treatment outcomes.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors, EGFR and PDGFRB, on the cell surface. Exhibits negligible protein tyrosine phosphatase activity at neutral pH. May act as a dominant-negative regulator of UBASH3B-dependent dephosphorylation. May inhibit dynamin-dependent endocytic pathways by functionally sequestering dynamin via its SH3 domain.
See full target information UBASH3A
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 13:982 PubMed36414640

2022

E2F1-initiated transcription of PRSS22 promotes breast cancer metastasis by cleaving ANXA1 and activating FPR2/ERK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Song,Hui Li,Ran-Ran Ma,Sen Liu,Guo-Hao Zhang,Xiang-Yu Guo,Rui-Nan Zhao,Xiao-Juan Wu,Kai Zhang,Peng Gao

Inflammation and regeneration 42:46 PubMed36324153

2022

IL-6 production through repression of UBASH3A gene via epigenetic dysregulation of super-enhancer in CD4 T cells in rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Kaoru Yamagata,Shingo Nakayamada,Tong Zhang,Anh Phuong Nguyen,Naoaki Ohkubo,Shigeru Iwata,Shigeaki Kato,Yoshiya Tanaka
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com