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AB155285

Anti-UBC4 antibody [EPR11032(B)]

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(1 Publication)

Rabbit Recombinant Monoclonal UBC4 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

PUBC1, UBC4, UBC5B, UBCH4, UBCH5B, UBE2D2, Ubiquitin-conjugating enzyme E2 D2, (E3-independent) E2 ubiquitin-conjugating enzyme D2, E2 ubiquitin-conjugating enzyme D2, Ubiquitin carrier protein D2, Ubiquitin-conjugating enzyme E2(17)KB 2, Ubiquitin-conjugating enzyme E2-17 kDa 2, Ubiquitin-protein ligase D2, p53-regulated ubiquitin-conjugating enzyme 1

1 Images
Western blot - Anti-UBC4 antibody [EPR11032(B)] (AB155285)
  • WB

Unknown

Western blot - Anti-UBC4 antibody [EPR11032(B)] (AB155285)

All lanes:

Western blot - Anti-UBC4 antibody [EPR11032(B)] (ab155285) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 16 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11032(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBC4 also known as Ubiquitin Conjugating Enzyme E2 D2 (Ube2d2) is an important component in the ubiquitination process. It weighs approximately 17.7 kDa and plays a pivotal role in labeling proteins for degradation by the proteasome. UBC4 is involved in catalyzing the transfer of activated ubiquitin from the E1 activating enzyme to a substrate protein. It is expressed in a wide range of tissues showing particularly high levels in testis and skeletal muscle. This wide expression pattern indicates a fundamental role in diverse cellular functions.
Biological function summary

UBC4 serves an important function in the regulation of protein homeostasis. It operates within the ubiquitin-proteasome system an essential cellular machinery that controls protein turnover. Although UBC4 acts independently it frequently associates with E3 ligases to target specific proteins for degradation. The enzyme thereby helps in maintaining cellular protein levels and regulating processes like cell cycle stress response and signal transduction.

Pathways

The ubiquitination mechanism involving UBC4 is critical in the cell cycle and stress response paths. In the cell cycle pathway UBC4 partners with relevant E3 ligases to ensure timely degradation of cyclins and other regulatory proteins facilitating proper cell division. In the stress response pathway UBC4 further collaborates with proteins such as HSP70 and HSP90 addressing damaged or misfolded proteins aiding cellular recovery from various stresses.

UBC4 exhibits significant connections to neurodegenerative disorders and cancers. In neurodegenerative conditions like Parkinson’s disease the malfunction of the ubiquitin-proteasome system contributes to protein aggregation and neuronal death. UBC4's role in these mechanisms makes it a critical factor in understanding the disease progression alongside proteins such as parkin and α-synuclein. In cancers irregularities in ubiquitination pathways involving UBC4 may lead to the persistence of oncoproteins and uncontrolled cellular proliferation highlighting its relevance to p53 and MDM2-associated pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed : 10329681, PubMed : 18042044, PubMed : 18703417, PubMed : 20061386, PubMed : 20403326, PubMed : 20525694, PubMed : 26475854, PubMed : 28322253). Catalyzes 'Lys-48'-linked polyubiquitination (PubMed : 10329681, PubMed : 18042044, PubMed : 18359941, PubMed : 18703417, PubMed : 20061386, PubMed : 20403326, PubMed : 20525694, PubMed : 26475854). Mediates the selective degradation of short-lived and abnormal proteins (PubMed : 10329681, PubMed : 18042044, PubMed : 18359941, PubMed : 18703417, PubMed : 20061386, PubMed : 20403326, PubMed : 20525694, PubMed : 26475854). Functions in the E6/E6-AP-induced ubiquitination of p53/TP53 (PubMed : 15280377). Mediates ubiquitination of PEX5 and SQSTM1 and autoubiquitination of STUB1 and TRAF6 (PubMed : 18359941, PubMed : 28322253). Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by RIGI in response to viral infection (PubMed : 18703417, PubMed : 20403326). Essential for viral activation of IRF3 (PubMed : 19854139).
See full target information UBE2D2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biochemistry and biophysics reports 29:101223 PubMed35146136

2022

Band 3/anion exchanger 1/solute carrier family 4 member 1 expression as determinant of cellular sensitivity to selenite exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Yasunori Fukumoto,Kemmu Matsuhashi,Yu-Ki Tanaka,Noriyuki Suzuki,Yasumitsu Ogra
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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