Rabbit Polyclonal UBE2C antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 10 publications.
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- Experimental & molecular medicine 54:2060-20762022Delineating the dynamic evolution from preneoplasia to invasive lung adenocarcinoma by integrating single-cell RNA sequencing and spatial transcriptomics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianfei Zhu,Yue Fan,Yanlu Xiong,Wenchen Wang,Jiakuan Chen,Yanmin Xia,Jie Lei,Li Gong,Shiquan Sun,Tao JiangPubMed 36434043
- Journal of gastrointestinal oncology 13:1444-14532022UBE2C promotes the progression of pancreatic cancer and glycolytic activity via EGFR stabilization-mediated PI3K-Akt pathway activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing-Zhu Cao,Gang Nie,Hao Hu,Xiao Zhang,Chen-Ming Ni,Zhi-Ping Huang,Guang-Lei Qiao,Liu OuyangPubMed 35837197
- Pathology oncology research : POR 27:6101592021LncRNA MALAT1 Regulating Lung Carcinoma Progression via the miR-491-5p/UBE2C Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuanjuan Dai,Ning Zhou,Rui Wu,Jing Du,Shuang Miao,Kaikai Gong,Lijuan Yang,Weiwei Chen,Xuelin Li,Chen Li,Yan WuPubMed 34257576
- Cell biology international 44:593-6022019Derlin-1 exhibits oncogenic activities and indicates an unfavorable prognosis in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinsheng Zeng,Qiuhong Tian,Zhimin Zeng,Jing Cai,Meiyu Ye,Yaxiong Liu,Anwen LiuPubMed 31670413
- Oncology reports 41:2987-29962019Antitumor effects of hsa‑miR661‑3p on non‑small cell lung cancer in vivo and in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingjing Lu et. al.PubMed 30896844
- Theranostics 9:2036-20552019UBE2C, Directly Targeted by miR-548e-5p, Increases the Cellular Growth and Invasive Abilities of Cancer Cells Interacting with the EMT Marker Protein Zinc Finger E-box Binding Homeobox 1/2 in NSCLC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan Jin et. al.PubMed 31037155
- EBioMedicine 35:204-2212018The miR 495-UBE2C-ABCG2/ERCC1 axis reverses cisplatin resistance by downregulating drug resistance genes in cisplatin-resistant non-small cell lung cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiwei Guo et. al.PubMed 30146342
- Oncotarget 7:63526-635362016A novel molecular and clinical staging model to predict survival for patients with esophageal squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Wang,Zhiwei Wang,Jun Zhao,Min Wei,Xinghua Zhu,Qi He,Tianlong Ling,Xiaoyan Chen,Ziang Cao,Yixin Zhang,Lei Liu,Minxin ShiPubMed 27556859
- Molecular cancer 9:1932010Gene expression profiling of mouse p53-deficient epidermal carcinoma defines molecular determinants of human cancer malignancy.
- Neoplasia (New York, N.Y.) 8:1062-712007Expression and effect of inhibition of the ubiquitin-conjugating enzyme E2C on esophageal adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJules Lin,Duna A Raoof,Zhuwen Wang,Mu-Yen Lin,Dafydd G Thomas,Joel K Greenson,Thomas J Giordano,Mark B Orringer,Andrew C Chang,David G Beer,Lin LinPubMed 17217624
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
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Target data
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
Alternative names
UBCH10, UBE2C, UBCH10, Ubiquitin-conjugating enzyme E2 C, (E3-independent) E2 ubiquitin-conjugating enzyme C, E2 ubiquitin-conjugating enzyme C, UbcH10, Ubiquitin carrier protein C, Ubiquitin-protein ligase C
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Rabbit Polyclonal UBE2C antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 10 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Specificity
This antibody recognises a band of the correct size (20 kDa) in Hela, A431, Jurkat, Jurkat nuclear and 293 lysates.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Add glycerol to a final volume of 50% for extra stability and aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UBE2C also known as ubiquitin-conjugating enzyme E2C or UBCH10 is an enzyme that plays a role in the ubiquitination process. Its molecular mass is approximately 20 kDa. UBE2C is an E2 ubiquitin-conjugating enzyme actively involved in marking proteins for degradation through the ubiquitin-proteasome system. It facilitates the transfer of ubiquitin to target proteins cooperating with E3 ligases. UBE2C shows expression in various tissues with high levels observed in proliferating cells such as those found in tumors.
- Biological function summary
UBE2C is involved in cell cycle regulation by targeting specific proteins for ubiquitination and degradation. It collaborates with the anaphase-promoting complex/cyclosome (APC/C) an important E3 ligase complex ensuring the proper progression of the cell cycle especially during the metaphase-anaphase transition. Through this action UBE2C aids in maintaining genomic stability by preventing the accumulation of proteins that can interfere with cell division.
- Pathways
The role of UBE2C is integral in the cell cycle and ubiquitin-proteasome pathways. The enzyme participates in the proper execution of the metaphase-anaphase transition and degradation of cell cycle regulators like cyclin B. UBE2C interacts with proteins such as CDC20 a co-activator of APC/C to ensure accurate coordination of cell division events. Its function in the ubiquitin-proteasome pathway emphasizes its role in maintaining protein homeostasis.
- Associated diseases and disorders
UBE2C's dysfunction or overexpression associates with cancer development and progression. Overexpression of UBE2C has been observed in various cancers including breast cancer and prostate cancer and may contribute to tumorigenesis by disrupting cell cycle control. UBE2C can interact with oncoproteins such as MYC further linking its dysregulation to cancer pathways. Understanding the relationships between UBE2C and cancer may offer insights into therapeutic targets for tumor treatment.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-UBE2C antibody (ab12290)
False colour image of Western blot: Anti-UBE2C antibody staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab12290 was shown to bind specifically to UBE2C. A band was observed at 20 kDa in wild-type HeLa cell lysates with no signal observed at this size in UBE2C knockout cell line Human UBE2C knockout HeLa cell line ab265032 (knockout cell lysate Human UBE2C knockout HeLa cell lysate ab257775). To generate this image, wild-type and UBE2C knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-UBE2C antibody (ab12290) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: UBE2C knockout HeLa cell lysate at 20 µg
Lane 3: WI-38 cell lysate at 20 µg
Lane 4: K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 20 µg
Lane 5: SW480 cell lysate at 20 µg
Lane 6: CACO2 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Western blot - Anti-UBE2C antibody (ab12290)
All lanes: Western blot - Anti-UBE2C antibody (ab12290) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 3: Jurkat nuclear extract lysate (ab14844) at 10 µg
Lane 4: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 5: HEK293 Human embryonic kidney cell line Whole Cell Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa, 55 kDa, 70 kDa
Immunocytochemistry/ Immunofluorescence - Anti-UBE2C antibody (ab12290)
ICC/IF image of ab12290 stained human HEK 293 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab12290, 1μg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HepG2 and MCF7 cells.
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