AB3935

Anti-UBE2C antibody

Name: Anti-UBE2C antibody (ab3935) | Abcam
Brand: Abcam Limited
SKU: AB3935

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(3 Publications)

Goat Polyclonal UBE2C antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human UBE2C aa 150 to C-terminus.

View Alternative Names

UBCH10, UBE2C, Ubiquitin-conjugating enzyme E2 C, (E3-independent) E2 ubiquitin-conjugating enzyme C, E2 ubiquitin-conjugating enzyme C, UbcH10, Ubiquitin carrier protein C, Ubiquitin-protein ligase C

2 Images

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Immunoprecipitation - Anti-UBE2C antibody (AB3935)

ab3935 staining approximately 20 kDa human UBE2 after immmunoprecipitation from HeLa whole cell lysate (10⁶ cells/ml) using 40μl of ab3935 saturated protein G dynabeads, by Western blot (ECL) :

Lane 1 = IP from Noc arrested HeLa cell extract (beads only).
Lane 2 = IP from Noc arrested HeLa cell extract (goat whole serum coupled to beads).
Lane 3 = IP from Noc arrested HeLa cell extract (ab3935 coupled to beads)
Lane 4 = Noc arrested HeLa whole cell lysate.

For a more detailed description, please click the reviews tab at the top of the page and see the review dated 25/02/03.

All lanes:

Immunoprecipitation - Anti-UBE2C antibody (ab3935)

Predicted band size: 20 kDa

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Western blot - Anti-UBE2C antibody (AB3935)

ab3935 at a 2.5μg/ml concentration staining approximately 20 kDa UBE2C by Western blot (ECL) :

Lane 1 = pCDNA3 transfected U2OS whole cell lysate (2μl).
Lane 2 = pCDNA3 MycUbcH10 transfected whole cell lysate (2μl).
Lane 3 = Nocodazole arrested HeLa cell whole cell lysate (20μl).
Lane 4 = Asynchronous HeLa cell whole cell lysate (20μl).

For a more detailed description, please click the reviews tab at the top of the page and see the review dated 25/02/03.

ab3935 at a 2.5µg/ml concentration staining approximately 20 kDa UBE2C by Western blot (ECL) :

Lane 1 = pCDNA3 transfected U2OS whole cell lysate (2µl).
Lane 2 = pCDNA3 MycUbcH10 transfected whole cell lysate (2µl).
Lane 3 = Nocodazole arrested HeLa cell whole cell lysate (20µl).
Lane 4 = Asynchronous HeLa cell whole cell lysate (20µl).

For a more detailed descri

All lanes:

Western blot - Anti-UBE2C antibody (ab3935)

Predicted band size: 20 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human UBE2C aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O00762

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>(See Abreview)</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2.5 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBE2C also known as ubiquitin-conjugating enzyme E2C or UBCH10 is an enzyme that plays a role in the ubiquitination process. Its molecular mass is approximately 20 kDa. UBE2C is an E2 ubiquitin-conjugating enzyme actively involved in marking proteins for degradation through the ubiquitin-proteasome system. It facilitates the transfer of ubiquitin to target proteins cooperating with E3 ligases. UBE2C shows expression in various tissues with high levels observed in proliferating cells such as those found in tumors.

Biological function summary

UBE2C is involved in cell cycle regulation by targeting specific proteins for ubiquitination and degradation. It collaborates with the anaphase-promoting complex/cyclosome (APC/C) an important E3 ligase complex ensuring the proper progression of the cell cycle especially during the metaphase-anaphase transition. Through this action UBE2C aids in maintaining genomic stability by preventing the accumulation of proteins that can interfere with cell division.

Pathways

The role of UBE2C is integral in the cell cycle and ubiquitin-proteasome pathways. The enzyme participates in the proper execution of the metaphase-anaphase transition and degradation of cell cycle regulators like cyclin B. UBE2C interacts with proteins such as CDC20 a co-activator of APC/C to ensure accurate coordination of cell division events. Its function in the ubiquitin-proteasome pathway emphasizes its role in maintaining protein homeostasis.

UBE2C's dysfunction or overexpression associates with cancer development and progression. Overexpression of UBE2C has been observed in various cancers including breast cancer and prostate cancer and may contribute to tumorigenesis by disrupting cell cycle control. UBE2C can interact with oncoproteins such as MYC further linking its dysregulation to cancer pathways. Understanding the relationships between UBE2C and cancer may offer insights into therapeutic targets for tumor treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.

See full target information UBE2C

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 33:2529-36 PubMed25760688

2015

Inhibition of microRNA-17/20a suppresses cell proliferation in gastric cancer by modulating UBE2C expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yingyi Zhang,Ting Han,Guo Wei,Yajie Wang

Gynecologic oncology 131:368-73 PubMed23911795

2013

Predicting response to the anti-estrogen fulvestrant in recurrent ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Peter A Argenta,Inhwa Um,Charlene Kay,David Harrison,Dana Faratian,Thanasak Sueblinvong,Melissa A Geller,Simon P Langdon

International journal of cancer 121:33-8 PubMed17354233

2007

Identification of overexpressed genes in hepatocellular carcinoma, with special reference to ubiquitin-conjugating enzyme E2C gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Keisuke Ieta,Eiki Ojima,Fumiaki Tanaka,Yoshito Nakamura,Naotsugu Haraguchi,Koshi Mimori,Hiroshi Inoue,Hiroyuki Kuwano,Masaki Mori

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UBE2C antibody

Immunoprecipitation - Anti-UBE2C antibody (AB3935)

Western blot - Anti-UBE2C antibody (AB3935)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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