Goat Polyclonal UBE2C antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human UBE2C aa 150 to C-terminus.
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Publications
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- Oncology reports 33:2529-362015Inhibition of microRNA-17/20a suppresses cell proliferation in gastric cancer by modulating UBE2C expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingyi Zhang et. al.PubMed 25760688
- Gynecologic oncology 131:368-732013Predicting response to the anti-estrogen fulvestrant in recurrent ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeter A Argenta,Inhwa Um,Charlene Kay,David Harrison,Dana Faratian,Thanasak Sueblinvong,Melissa A Geller,Simon P LangdonPubMed 23911795
- International journal of cancer 121:33-82007Identification of overexpressed genes in hepatocellular carcinoma, with special reference to ubiquitin-conjugating enzyme E2C gene expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeisuke Ieta,Eiki Ojima,Fumiaki Tanaka,Yoshito Nakamura,Naotsugu Haraguchi,Koshi Mimori,Hiroshi Inoue,Hiroyuki Kuwano,Masaki MoriPubMed 17354233
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human UBE2C aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O00762
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes (See Abreview) |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.5 µg/mL | Notes A 1 hour primary incubation is recommended for this product. |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
Alternative names
UBCH10, UBE2C, Ubiquitin-conjugating enzyme E2 C, (E3-independent) E2 ubiquitin-conjugating enzyme C, E2 ubiquitin-conjugating enzyme C, UbcH10, Ubiquitin carrier protein C, Ubiquitin-protein ligase C
Recommended products
Goat Polyclonal UBE2C antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human UBE2C aa 150 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human UBE2C aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O00762
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UBE2C also known as ubiquitin-conjugating enzyme E2C or UBCH10 is an enzyme that plays a role in the ubiquitination process. Its molecular mass is approximately 20 kDa. UBE2C is an E2 ubiquitin-conjugating enzyme actively involved in marking proteins for degradation through the ubiquitin-proteasome system. It facilitates the transfer of ubiquitin to target proteins cooperating with E3 ligases. UBE2C shows expression in various tissues with high levels observed in proliferating cells such as those found in tumors.
- Biological function summary
UBE2C is involved in cell cycle regulation by targeting specific proteins for ubiquitination and degradation. It collaborates with the anaphase-promoting complex/cyclosome (APC/C) an important E3 ligase complex ensuring the proper progression of the cell cycle especially during the metaphase-anaphase transition. Through this action UBE2C aids in maintaining genomic stability by preventing the accumulation of proteins that can interfere with cell division.
- Pathways
The role of UBE2C is integral in the cell cycle and ubiquitin-proteasome pathways. The enzyme participates in the proper execution of the metaphase-anaphase transition and degradation of cell cycle regulators like cyclin B. UBE2C interacts with proteins such as CDC20 a co-activator of APC/C to ensure accurate coordination of cell division events. Its function in the ubiquitin-proteasome pathway emphasizes its role in maintaining protein homeostasis.
- Associated diseases and disorders
UBE2C's dysfunction or overexpression associates with cancer development and progression. Overexpression of UBE2C has been observed in various cancers including breast cancer and prostate cancer and may contribute to tumorigenesis by disrupting cell cycle control. UBE2C can interact with oncoproteins such as MYC further linking its dysregulation to cancer pathways. Understanding the relationships between UBE2C and cancer may offer insights into therapeutic targets for tumor treatment.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-UBE2C antibody (ab3935)
ab3935 at a 2.5μg/ml concentration staining approximately 20 kDa UBE2C by Western blot (ECL):
Lane 1 = pCDNA3 transfected U2OS whole cell lysate (2μl).
Lane 2 = pCDNA3 MycUbcH10 transfected whole cell lysate (2μl).
Lane 3 = Nocodazole arrested HeLa cell whole cell lysate (20μl).
Lane 4 = Asynchronous HeLa cell whole cell lysate (20μl).
For a more detailed description, please click the reviews tab at the top of the page and see the review dated 25/02/03.ab3935 at a 2.5µg/ml concentration staining approximately 20 kDa UBE2C by Western blot (ECL):
Lane 1 = pCDNA3 transfected U2OS whole cell lysate (2µl).
Lane 2 = pCDNA3 MycUbcH10 transfected whole cell lysate (2µl).
Lane 3 = Nocodazole arrested HeLa cell whole cell lysate (20µl).
Lane 4 = Asynchronous HeLa cell whole cell lysate (20µl).
For a more detailed descriAll lanes: Western blot - Anti-UBE2C antibody (ab3935)
Predicted band size: 20 kDa
Immunoprecipitation - Anti-UBE2C antibody (ab3935)
ab3935 staining approximately 20 kDa human UBE2 after immmunoprecipitation from HeLa whole cell lysate (106 cells/ml) using 40μl of ab3935 saturated protein G dynabeads, by Western blot (ECL):
Lane 1 = IP from Noc arrested HeLa cell extract (beads only).
Lane 2 = IP from Noc arrested HeLa cell extract (goat whole serum coupled to beads).
Lane 3 = IP from Noc arrested HeLa cell extract (ab3935 coupled to beads)
Lane 4 = Noc arrested HeLa whole cell lysate.For a more detailed description, please click the reviews tab at the top of the page and see the review dated 25/02/03.
All lanes: Immunoprecipitation - Anti-UBE2C antibody (ab3935)
Predicted band size: 20 kDa
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