AB249927

Anti-UBE2D3 antibody [EPR13001(B)] - BSA and Azide free

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal UBE2D3 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

UBC5C, UBCH5C, UBE2D3, Ubiquitin-conjugating enzyme E2 D3, (E3-independent) E2 ubiquitin-conjugating enzyme D3, E2 ubiquitin-conjugating enzyme D3, Ubiquitin carrier protein D3, Ubiquitin-conjugating enzyme E2(17)KB 3, Ubiquitin-conjugating enzyme E2-17 kDa 3, Ubiquitin-protein ligase D3

2 Images

Supplier Data

Immunoprecipitation - Anti-UBE2D3 antibody [EPR13001(B)] - BSA and Azide free (AB249927)

This data was developed using ab176568, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from 293T cell lysate labeling UBE2D3 with ab176568 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-UBE2D3 antibody [EPR13001(B)] - N-terminal (<a href='/en-us/products/primary-antibodies/ube2d3-antibody-epr13001b-n-terminal-ab176568'>ab176568</a>)

Predicted band size: 16 kDa

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Supplier Data

Western blot - Anti-UBE2D3 antibody [EPR13001(B)] - BSA and Azide free (AB249927)

This data was developed using ab176568, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-UBE2D3 antibody [EPR13001(B)] - N-terminal (<a href='/en-us/products/primary-antibodies/ube2d3-antibody-epr13001b-n-terminal-ab176568'>ab176568</a>) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 16 kDa

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Unconjugated

Anti-UBE2D3 antibody [EPR13001(B)] - N-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13001(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 6 continuous identical amino acids with UBE2D1,UBE2D2 and UBE2D4. Cross-reactivity with these proteins have not been confirmed experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab249927 is the carrier-free version of ab176568.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBE2D3 also known as UbcH5c or E2-17k is a ubiquitin-conjugating enzyme. It plays a critical role in the ubiquitination process by interacting with ligase enzymes adding ubiquitin to target proteins. This protein has a molecular mass of approximately 16 kDa. UBE2D3 is widely expressed in various tissues indicating its role in several cellular functions.

Biological function summary

UBE2D3 acts as an important component in protein degradation and regulation. It participates in the ubiquitin-proteasome system which controls protein levels in cells. UBE2D3 forms part of larger protein complexes such as the SCF (Skp Cullin F-box containing) complex contributing to the regulation of cell cycle and signal transduction. This interaction helps control cell division and response to external signals.

Pathways

UBE2D3 plays an important role in the NF-kB signaling and DNA damage response pathways. It interacts with proteins such as IKK (IκB kinase) to regulate inflammation and immune response. Additionally UBE2D3's involvement in the DNA repair pathway supports maintaining genome stability by collaborating with other proteins like BRCA1 which is essential for repairing DNA double-strand breaks.

UBE2D3 shows a connection to cancer and neurodegenerative diseases. Its role in the NF-kB signaling pathway links it to cancer development due to its regulation of genes involved in cell growth and survival. In neurodegenerative disorders such as Alzheimer's disease abnormal ubiquitination due to UBE2D3 dysfunction may lead to protein aggregation affecting normal cellular activities. Its interaction with proteins like p53 a well-known tumor suppressor illustrates its importance in disease progression and regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed : 15247280, PubMed : 15496420, PubMed : 18284575, PubMed : 20061386, PubMed : 21532592, PubMed : 28322253). In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination (PubMed : 15247280, PubMed : 15496420, PubMed : 18284575, PubMed : 20061386, PubMed : 21532592). Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation (PubMed : 20347421). Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin (PubMed : 10329681). Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin (PubMed : 10329681). Also acts as an initiator E2, in conjunction with RNF8, for the priming of PCNA (PubMed : 18948756). Monoubiquitination of PCNA, and its subsequent polyubiquitination, are essential events in the operation of the DNA damage tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase (PubMed : 18948756). Associates with the BRCA1/BARD1 E3 ligase complex to perform ubiquitination at DNA damage sites following ionizing radiation leading to DNA repair (PubMed : 16628214). Targets DAPK3 for ubiquitination which influences promyelocytic leukemia protein nuclear body (PML-NB) formation in the nucleus (PubMed : 18515077). In conjunction with the MDM2 and TOPORS E3 ligases, functions ubiquitination of p53/TP53 (PubMed : 12646252, PubMed : 15280377). In conjunction with the CBL E3 ligase, targets EGFR for polyubiquitination at the plasma membrane as well as during its internalization and transport on endosomes (PubMed : 18508924). In conjunction with the STUB1 E3 quality control E3 ligase, ubiquitinates unfolded proteins to catalyze their immediate destruction (PubMed : 11743028). Together with RNF135, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIGI to activate the downstream signaling pathway that leads to interferon beta production (PubMed : 28469175). Together with ZNF598, catalyzes ubiquitination of 40S ribosomal proteins in response to ribosome collisions (PubMed : 28685749). In cooperation with the GATOR2 complex, catalyzes 'Lys-6'-linked ubiquitination of NPRL2 (PubMed : 36528027).

See full target information UBE2D3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 7:357 PubMed34785642

2021

UBE2S promotes cell chemoresistance through PTEN-AKT signaling in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Gui,Sicai Zhang,Yongzi Xu,Hongwei Zhang,Ying Zhu,Lianbao Kong

Molecular cancer 20:123 PubMed34579723

2021

p113 isoform encoded by CUX1 circular RNA drives tumor progression via facilitating ZRF1/BRD4 transactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Yang,Anpei Hu,Yanhua Guo,Jianqun Wang,Dan Li,Xiaojing Wang,Shikai Jin,Boling Yuan,Shuang Cai,Yi Zhou,Qilan Li,Guo Chen,Haiyang Gao,Liduan Zheng,Qiangsong Tong

Frontiers in oncology 11:674286 PubMed34195079

2021

UBE2D3 Activates SHP-2 Ubiquitination to Promote Glycolysis and Proliferation of Glioma Regulating STAT3 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenjiang Pan,Jing Bao,Liujun Zhang,Shepeng Wei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UBE2D3 antibody [EPR13001(B)] - BSA and Azide free

Immunoprecipitation - Anti-UBE2D3 antibody [EPR13001(B)] - BSA and Azide free (AB249927)

Western blot - Anti-UBE2D3 antibody [EPR13001(B)] - BSA and Azide free (AB249927)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise