Anti-UBE2D3 antibody [EPR13001(B)] - N-terminal

• IP

Supplier Data

Immunoprecipitation - Anti-UBE2D3 antibody [EPR13001(B)] - N-terminal (AB176568)

Western blot analysis on immunoprecipitation pellet from 293T cell lysate labeling UBE2D3 with ab176568 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-UBE2D3 antibody [EPR13001(B)] - N-terminal (ab176568)

Predicted band size: 16 kDa

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• WB

Supplier Data

Western blot - Anti-UBE2D3 antibody [EPR13001(B)] - N-terminal (AB176568)

All lanes:

Western blot - Anti-UBE2D3 antibody [EPR13001(B)] - N-terminal (ab176568) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 16 kDa

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• WB

CiteAb

Western blot - Anti-UBE2D3 antibody [EPR13001(B)] - N-terminal (AB176568)

UBE2D3 western blot using anti-UBE2D3 antibody [EPR13001(B)] - N-terminal ab176568. Publication image and figure legend from Shi, Y., Yuan, B., et al., 2017, Nat Commun, PubMed 28469175.

ab176568 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab176568 please see the product overview.

Ub2D3 and Ube2N are required for RIG-I and MAVS activation on virus infection in HEK293T cells.(a,b) The cell-free assay containing vRNA and purified components (E1, Ub, RIG-I and Riplet) was performed as described in Fig. 2b, except that S100 fraction was substituted with purified E2 recombinant proteins as indicated. Each E2 protein was analysed at three different concentrations. Lane 13 did not contain E2 proteins (-E2). (c) HEK293T cells (wild-type and knockout lines) were transfected with or without si-Ube2D3 RNAs. Seventy two hours after transfection, the cells were infected with VSV. Twelve hours post virus infection, the cells were collected for measuring IFN induction by qPCR. *p<0.05 and ***p<0.001. (d,e) Cells were treated as described in c. P5 fractions were isolated to examine MAVS aggregation (d), and whole cell lysate were used to analyse knockdown or knockout effect in genes as indicated (e). The original full blot for (e) can be found in Supplementary Fig. 8d. (f) si-Ube2D3 RNAs were transfected into HEK293T (Ube2N-/-) cells. Seventy two hours after siRNA transfection, the cells were further transfected with pcDNA-flag-Ube2D3 or pcDNA-flag-Ube2N as indicated. Twenty four hours after transfection of pcDNA plasmids, the cells were infected with VSV for another twelve hours. The cells were then collected for analysis of MAVS aggregation. See also Supplementary Fig. 3f.

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