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AB101371

Anti-Ube2G1 antibody

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(1 Publication)

Rabbit Polyclonal Ube2G1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human UBE2G1 aa 1 to C-terminus.

View Alternative Names

UBE2G, UBE2G1, Ubiquitin-conjugating enzyme E2 G1, E2 ubiquitin-conjugating enzyme G1, E217K, UBC7, Ubiquitin carrier protein G1, Ubiquitin-protein ligase G1

1 Images
Western blot - Anti-Ube2G1 antibody (AB101371)
  • WB

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Western blot - Anti-Ube2G1 antibody (AB101371)

12% SDS Page

All lanes:

Western blot - Anti-Ube2G1 antibody (ab101371) at 1/1000 dilution

All lanes:

HCT116 whole cell lysate at 30 µg

Predicted band size: 11 kDa,19 kDa,20 kDa,21 kDa,22 kDa,23 kDa,24 kDa,26 kDa,27 kDa,28 kDa,29 kDa,30 kDa,31 kDa,32 kDa,34 kDa,35 kDa,37 kDa,38 kDa,40 kDa,41 kDa,42 kDa,43 kDa,47 kDa,54 kDa,56 kDa,70 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human UBE2G1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P62253

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Secondary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ube2G1 also known as ubiquitin-conjugating enzyme E2 G1 plays an important role in the ubiquitination process. This protein with a molecular mass of about 23 kDa is involved in tagging substrates with ubiquitin for proteasomal degradation—an important cellular mechanism that regulates protein levels and quality. Ube2G1 expresses in various tissues including the liver and central nervous system highlighting its broad role in maintaining cellular functions.
Biological function summary

Ube2G1's primary function supports protein homeostasis and cellular stress responses. It collaborates with E3 ubiquitin ligases in a complex to mediate the transfer of ubiquitin molecules to their targets. This activity is essential in controlling various cellular processes such as cell cycle regulation DNA repair and signal transduction. By participating in these functions Ube2G1 assures that the cellular environment remains stable and efficient.

Pathways

Ube2G1 has pivotal involvement in the ubiquitin-proteasome pathway which is significant for the regulated degradation of misfolded or damaged proteins. Additionally it interacts with components of the endoplasmic reticulum-associated degradation (ERAD) pathway working alongside proteins like Derlin-1 to eliminate faulty proteins from the ER. Through these pathways Ube2G1 contributes to cellular health by preventing protein aggregation and dysfunction.

Improper function of Ube2G1 can have serious implications for neurological conditions such as Alzheimer's disease due to the accumulation of misfolded proteins. Additionally cancer progression is linked to disruptions in protein degradation mechanisms involving Ube2G1 as it influences cell cycle and apoptosis. In cancer Ube2G1 can interact with tumor suppressor proteins like p53 affecting their stability and activity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4.
See full target information UBE2G1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:675 PubMed39277583

2024

Inhibiting S-palmitoylation arrests metastasis by relocating Rap2b from plasma membrane in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangli Zhu,Xize Cao,Zhenshuai Chen,Birou Lai,Lingling Xi,Jinghang Zhang,Shaohui Zhu,Shiqian Qi,Yinming Liang,Fei Cao,Binhui Zhou,Yu Song,Sheng Jiang,Tianyu Wang,Xiaohong Kang,Eryan Kong
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Product promise

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