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AB235790

Anti-Ube2G2 antibody

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(2 Publications)

Rabbit Polyclonal Ube2G2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human UBE2G2.

View Alternative Names

UBC7, UBE2G2, Ubiquitin-conjugating enzyme E2 G2, E2 ubiquitin-conjugating enzyme G2, Ubiquitin carrier protein G2, Ubiquitin-protein ligase G2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube2G2 antibody (AB235790)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube2G2 antibody (AB235790)

Paraffin-embedded human pancreatic tissue stained for Ube2G2 using ab235790 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube2G2 antibody (AB235790)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube2G2 antibody (AB235790)

Paraffin-embedded human colon cancer tissue stained for Ube2G2 using ab235790 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-Ube2G2 antibody (AB235790)
  • WB

Supplier Data

Western blot - Anti-Ube2G2 antibody (AB235790)

All lanes:

Western blot - Anti-Ube2G2 antibody (ab235790) at 1/500 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 3:

A549 (human lung carcinoma cell line) whole cell lysate

Lane 4:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 5:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Lane 6:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 19 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human UBE2G2.

P60604

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ube2G2 also known as Ubiquitin-conjugating enzyme E2 G2 is involved in the ubiquitin-proteasome pathway. This enzyme functions by facilitating the transfer of ubiquitin from E1 activating enzymes to target proteins marking them for degradation. It has a molecular mass of approximately 21 kDa and is expressed in various tissues with noticeable presence in the heart and skeletal muscle. The activity of Ube2G2 is essential for maintaining protein homeostasis in cells.
Biological function summary

Ube2G2 interacts as a part of the ubiquitin-conjugating system forming complexes with E3 ligases like gp78. These complexes determine substrate specificity and enhance the degradation of misfolded or damaged proteins. The function of Ube2G2 in protein degradation is important for cellular processes including cell cycle regulation and stress responses. The enzyme therefore contributes to maintaining cellular integrity by preventing the accumulation of nonfunctional proteins.

Pathways

Ube2G2 plays an integral role in the endoplasmic reticulum-associated degradation (ERAD) pathway which orchestrates the removal of defective proteins from the endoplasmic reticulum. Ube2G2 collaborates with the E3 ligase gp78 and these together facilitate the ubiquitination and subsequent proteasomal degradation of target substrates. Additionally Ube2G2 influences calcium-related signaling pathways impacting cellular calcium homeostasis.

Ube2G2's dysfunction is linked with neurological disorders and cancer. Impairments in Ube2G2 activity may lead to defective ERAD contributing to neurodegenerative disorders through the accumulation of toxic protein aggregates. In cancer altered Ube2G2 expression affects the degradation of oncogenic proteins implicating it in tumorigenesis. In these contexts its interaction with proteins like gp78 becomes significant in understanding how cellular degradation pathways are disrupted.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed : 20061386). In vitro catalyzes 'Lys-48'-linked polyubiquitination (PubMed : 20061386). Involved in endoplasmic reticulum-associated degradation (ERAD) (PubMed : 22607976). Required for sterol-induced ubiquitination of 3-hydroxy-3-methylglutaryl coenzyme A reductase and its subsequent proteasomal degradation (PubMed : 23223569).
See full target information UBE2G2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:109 PubMed40119335

2025

TMEM71 is crucial for cell proliferation in lower-grade glioma and is linked to unfavorable prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiabao Xie,Wanli Yu,Shikai Gui,Lunshan Peng,Juexian Xiao,Haitao Luo,Zujue Cheng

Biomedicines 10: PubMed35625704

2022

Arsenic Activates the ER Stress-Associated Unfolded Protein Response via the Activating Transcription Factor 6 in Human Bronchial Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Priya Wadgaonkar,Zhuoyue Bi,Junmei Wan,Yao Fu,Qian Zhang,Bandar Almutairy,Wenxuan Zhang,Yiran Qiu,Chitra Thakur,Maik Hüttemann,Fei Chen
View all publications
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Product promise

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