AB174296

Anti-Ube2G2 antibody [EPR9248(2)]

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal Ube2G2 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

UBC7, UBE2G2, Ubiquitin-conjugating enzyme E2 G2, E2 ubiquitin-conjugating enzyme G2, Ubiquitin carrier protein G2, Ubiquitin-protein ligase G2

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Ube2G2 antibody [EPR9248(2)] (AB174296)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Ube2G2 antibody [EPR9248(2)] (AB174296)

Immunofluorescent analysis of HeLa cells labeling Ube2G2 with ab174296 at 1/100 dilution (red). DAPI nuclear staining (blue).

Supplier Data

Immunoprecipitation - Anti-Ube2G2 antibody [EPR9248(2)] (AB174296)

Western blot analysis on immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab174296 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)

Predicted band size: 19 kDa

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Supplier Data

Western blot - Anti-Ube2G2 antibody [EPR9248(2)] (AB174296)

All lanes:

Western blot - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 10 µg

Lane 2:

Rat testis tissue lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Lane 5:

K562 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 19 kDa

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Carrier free

Anti-Ube2G2 antibody [EPR9248(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9248(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ube2G2 also known as Ubiquitin-conjugating enzyme E2 G2 is involved in the ubiquitin-proteasome pathway. This enzyme functions by facilitating the transfer of ubiquitin from E1 activating enzymes to target proteins marking them for degradation. It has a molecular mass of approximately 21 kDa and is expressed in various tissues with noticeable presence in the heart and skeletal muscle. The activity of Ube2G2 is essential for maintaining protein homeostasis in cells.

Biological function summary

Ube2G2 interacts as a part of the ubiquitin-conjugating system forming complexes with E3 ligases like gp78. These complexes determine substrate specificity and enhance the degradation of misfolded or damaged proteins. The function of Ube2G2 in protein degradation is important for cellular processes including cell cycle regulation and stress responses. The enzyme therefore contributes to maintaining cellular integrity by preventing the accumulation of nonfunctional proteins.

Pathways

Ube2G2 plays an integral role in the endoplasmic reticulum-associated degradation (ERAD) pathway which orchestrates the removal of defective proteins from the endoplasmic reticulum. Ube2G2 collaborates with the E3 ligase gp78 and these together facilitate the ubiquitination and subsequent proteasomal degradation of target substrates. Additionally Ube2G2 influences calcium-related signaling pathways impacting cellular calcium homeostasis.

Ube2G2's dysfunction is linked with neurological disorders and cancer. Impairments in Ube2G2 activity may lead to defective ERAD contributing to neurodegenerative disorders through the accumulation of toxic protein aggregates. In cancer altered Ube2G2 expression affects the degradation of oncogenic proteins implicating it in tumorigenesis. In these contexts its interaction with proteins like gp78 becomes significant in understanding how cellular degradation pathways are disrupted.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed : 20061386). In vitro catalyzes 'Lys-48'-linked polyubiquitination (PubMed : 20061386). Involved in endoplasmic reticulum-associated degradation (ERAD) (PubMed : 22607976). Required for sterol-induced ubiquitination of 3-hydroxy-3-methylglutaryl coenzyme A reductase and its subsequent proteasomal degradation (PubMed : 23223569).

See full target information UBE2G2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:2698 PubMed37164963

2023

Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Lan,Sophie Wilhelmina van Leur,Julia Ayano Fernando,Ho Him Wong,Martin Kampmann,Lewis Siu,Jingshu Zhang,Mingyuan Li,John M Nicholls,Sumana Sanyal

Cell reports 41:111675 PubMed36417855

2022

Dynamic quality control machinery that operates across compartmental borders mediates the degradation of mammalian nuclear membrane proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Pei-Ling Tsai,Christopher J F Cameron,Maria Fernanda Forni,Renee R Wasko,Brigitte S Naughton,Valerie Horsley,Mark B Gerstein,Christian Schlieker

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 26:183-193 PubMed35477546

2022

Differentially expressed mRNAs and their upstream miR-491-5p in patients with coronary atherosclerosis as well as the function of miR-491-5p in vascular smooth muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Ding,Quanhua Pan,Long Qian,Chuanxian Hu

PLoS pathogens 13:e1006674 PubMed28985237

2017

Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Wang,Bei Wang,He Huang,Chongyang Zhang,Yuanmei Zhu,Bin Pei,Chaofei Cheng,Lei Sun,Jianwei Wang,Qi Jin,Zhendong Zhao

Journal of cell science 130:2883-2892 PubMed28743740

2017

Multiple E2 ubiquitin-conjugating enzymes regulate human cytomegalovirus US2-mediated immunoreceptor downregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Michael L van de Weijer,Anouk B C Schuren,Dick J H van den Boomen,Arend Mulder,Frans H J Claas,Paul J Lehner,Robert Jan Lebbink,Emmanuel J H J Wiertz

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ube2G2 antibody [EPR9248(2)]

Immunocytochemistry/ Immunofluorescence - Anti-Ube2G2 antibody [EPR9248(2)] (AB174296)

Immunoprecipitation - Anti-Ube2G2 antibody [EPR9248(2)] (AB174296)

Western blot - Anti-Ube2G2 antibody [EPR9248(2)] (AB174296)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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