AB21193

Anti-UBE2I / UBC9 antibody

Name: Anti-UBE2I / UBC9 antibody (ab21193) | Abcam
Brand: Abcam Limited
SKU: AB21193
Rating: 5 (2 reviews)

(2 Reviews)

(20 Publications)

Goat Polyclonal UBE2I / UBC9 antibody. Suitable for WB, IHC-P and reacts with Rat, Human samples. Cited in 20 publications. Immunogen corresponding to Synthetic Peptide within Human UBE2I aa 1-50.

View Alternative Names

UBC9, UBCE9, UBE2I, SUMO-conjugating enzyme UBC9, RING-type E3 SUMO transferase UBC9, SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, p18

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2I / UBC9 antibody (AB21193)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2I / UBC9 antibody (AB21193)

ab21193 (0.3ug/ml) staining human UBE2I in human kidney by immunohistochemistry using paraffin embedded tissue. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Unknown

Western blot - Anti-UBE2I / UBC9 antibody (AB21193)

Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-UBE2I / UBC9 antibody (ab21193) at 0.1 µg/mL

All lanes:

Human kidney lysate at 35 µg

Predicted band size: 18 kDa

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Western blot - Anti-UBE2I / UBC9 antibody (AB21193)

All lanes:

Western blot - Anti-UBE2I / UBC9 antibody (ab21193) at 0.3 µg/mL

All lanes:

Rat ovary lysate at 35 µg

Predicted band size: 18 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human UBE2I aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P63279

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "A 1 hour primary incubation is recommended for this product. Transfer onto PVDF membrane and block in 3 % milk. Incubate primary antibody with membrane at room temperature.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "A 1 hour primary incubation is recommended for this product. Transfer onto PVDF membrane and block in 3 % milk. Incubate primary antibody with membrane at room temperature.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Product is very sensitive to freeze/thawing therefore this should be avoided. Store at 4C for short term use and -20C for long term storage in small aliquots.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBE2I also known as UBC9 is a small ubiquitin-related modifier (SUMO) conjugating enzyme. It plays an important role in the SUMOylation process which modifies proteins by attaching sumo proteins to them altering their function. This protein has a molecular mass of approximately 18 kDa. UBE2I is expressed broadly across various tissues demonstrating its significance in cellular processes.

Biological function summary

The SUMO conjugating enzyme UBE2I is part of the SUMOylation complex which includes the SUMO E1 enzyme and SUMO E3 ligase. It facilitates the attachment of SUMO proteins to target proteins which can influence protein stability localization and interaction with other proteins. This modification usually regulates important cellular functions such as transcription DNA repair and cell cycle progression.

Pathways

Proteins modified by UBE2I participate in significant biological pathways such as the DNA damage response and cell cycle regulation. In the DNA damage response UBE2I works with proteins such as p53 and BRCA1 to maintain genomic stability. In cell cycle pathways interactions with proteins like cyclin D1 and CDK inhibitors help control cellular proliferation by SUMOylating key regulatory proteins.

UBE2I has been implicated in cancer and neurodegenerative diseases. Altered SUMOylation by UBE2I can lead to the dysfunction of tumor suppressor proteins like p53 contributing to cancer development. In neurodegenerative diseases abnormal SUMOylation might affect proteins like tau which can lead to the progression of conditions such as Alzheimer's disease. The role of UBE2I in these disorders highlights it as a potential therapeutic target in addressing these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity (PubMed : 26620705). Sumoylates SHMT1 at 'Lys-38' or 'Lys-39' leading to RAN-dependent nuclear import of SHMT1 (PubMed : 17446168). Also sumoylates TYMS and DHFR (PubMed : 18067453).

See full target information UBE2I

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112555 PubMed40487451

2025

Targeting SUMOylation in ovarian cancer: Sensitivity, resistance, and the role of MYC.

Applications

Unspecified application

Species

Unspecified reactive species

Samantha Littler,Bethany M Barnes,Rhys Owen,Louisa Nelson,Anthony Tighe,I-Hsuan Lin,Hugh C Osborne,Christine K Schmidt,Joanne C McGrail,Stephen S Taylor

NPJ precision oncology 8:258 PubMed39521913

2024

RNF4 mediated degradation of PDHA1 promotes colorectal cancer metabolism and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jierong Chen,Zi-Yue Li,Guansheng Zheng,Lixue Cao,Yun-Miao Guo,Qizhou Lian,Bing Gu,Cai-Feng Yue

Cell reports 34:108929 PubMed33789095

2021

Molecular mechanisms of APC/C release from spindle assembly checkpoint inhibition by APC/C SUMOylation.

Applications

Unspecified application

Species

Unspecified reactive species

Stanislau Yatskevich,Jessie S Kroonen,Claudio Alfieri,Thomas Tischer,Anna C Howes,Linda Clijsters,Jing Yang,Ziguo Zhang,Kaige Yan,Alfred C O Vertegaal,David Barford

Evolution; international journal of organic evolution 74:2451-2464 PubMed32989734

2020

Short-term heritable variation overwhelms 200 generations of mutational variance for metabolic traits in Caenorhabditis elegans.

Applications

Unspecified application

Species

Unspecified reactive species

Lindsay M Johnson,Olivia J Smith,Daniel A Hahn,Charles F Baer

The Journal of biological chemistry 295:8350-8362 PubMed32350109

2020

Multisite SUMOylation restrains DNA polymerase η interactions with DNA damage sites.

Applications

Unspecified application

Species

Unspecified reactive species

Claire Guérillon,Stine Smedegaard,Ivo A Hendriks,Michael L Nielsen,Niels Mailand

Frontiers in cellular neuroscience 13:486 PubMed31749687

2019

Super Resolution Microscopy of SUMO Proteins in Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Luca Colnaghi,Luca Russo,Carmina Natale,Elena Restelli,Alfredo Cagnotto,Mario Salmona,Roberto Chiesa,Luana Fioriti

Journal of molecular neuroscience : MN 69:391-398 PubMed31267313

2019

Inhibition of Ubc9-Induced CRMP2 SUMOylation Disrupts Glioblastoma Cell Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Leilei Wang,Suzhen Ji

International journal of human genetics and genetic disorders 1:1-8 PubMed33860286

2019

A Provocative Molecular Link between Mammographic Density and BRCA1-loss associated TNBC.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyao Xu,Gbinigie Olusola,Alexus Footman,Nora Hansen,Aswathy Miriam Cheriyan,Krishna Koganti,Vaishali Reddy,Samir Yezdani,Vikram Eddy,Henry De'smond,Nicolas Bakinde,Joel Okoli,Gabriela Oprea,Kathleen Gundry,E Shyam P Reddy,Veena N Rao

Cell chemical biology 26:278-288.e6 PubMed30581133

2018

Allosteric Inhibition of Ubiquitin-like Modifications by a Class of Inhibitor of SUMO-Activating Enzyme.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Jia Li,Li Du,Jianghai Wang,Ramir Vega,Terry D Lee,Yunan Miao,Grace Aldana-Masangkay,Eric R Samuels,Baozong Li,S Xiaohu Ouyang,Sharon A Colayco,Ekaterina V Bobkova,Daniela B Divlianska,Eduard Sergienko,Thomas D Y Chung,Marwan Fakih,Yuan Chen

Journal of molecular cell biology 11:356-370 PubMed29868881

2018

Muscle RING-finger protein-1 (MuRF1) functions and cellular localization are regulated by SUMO1 post-translational modification.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriel Heras,Arvind Venkat Namuduri,Leonardo Traini,Ganna Shevchenko,Alexander Falk,Sara Bergström Lind,Mi Jia,Geng Tian,Stefano Gastaldello

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UBE2I / UBC9 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2I / UBC9 antibody (AB21193)

Western blot - Anti-UBE2I / UBC9 antibody (AB21193)

Western blot - Anti-UBE2I / UBC9 antibody (AB21193)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (20)

Product promise