Name: Anti-UBE2I / UBC9 antibody [EP2938Y]
SKU: ab75854
Rating: 4 (8 reviews)

Rabbit Recombinant Monoclonal UBE2I / UBC9 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 42 publications.

Images

Immunoprecipitation - Anti-UBE2I / UBC9 antibody [EP2938Y] (AB75854), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Tested	Expected	Tested	Expected	Expected
Rat	Tested	Expected	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/250	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/2000	Notes -
Species Rat	Dilution info 1/2000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000 - 1/10000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information UBE2I

Function

Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity (PubMed:26620705).

Alternative names

UBC9, UBCE9, UBE2I, SUMO-conjugating enzyme UBC9, RING-type E3 SUMO transferase UBC9, SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, p18

Recommended products

Rabbit Recombinant Monoclonal UBE2I / UBC9 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 42 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EP2938Y
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

UBE2I also known as UBC9 is a small ubiquitin-related modifier (SUMO) conjugating enzyme. It plays an important role in the SUMOylation process which modifies proteins by attaching sumo proteins to them altering their function. This protein has a molecular mass of approximately 18 kDa. UBE2I is expressed broadly across various tissues demonstrating its significance in cellular processes.

Biological function summary

The SUMO conjugating enzyme UBE2I is part of the SUMOylation complex which includes the SUMO E1 enzyme and SUMO E3 ligase. It facilitates the attachment of SUMO proteins to target proteins which can influence protein stability localization and interaction with other proteins. This modification usually regulates important cellular functions such as transcription DNA repair and cell cycle progression.

Pathways

Proteins modified by UBE2I participate in significant biological pathways such as the DNA damage response and cell cycle regulation. In the DNA damage response UBE2I works with proteins such as p53 and BRCA1 to maintain genomic stability. In cell cycle pathways interactions with proteins like cyclin D1 and CDK inhibitors help control cellular proliferation by SUMOylating key regulatory proteins.

Associated diseases and disorders

UBE2I has been implicated in cancer and neurodegenerative diseases. Altered SUMOylation by UBE2I can lead to the dysfunction of tumor suppressor proteins like p53 contributing to cancer development. In neurodegenerative diseases abnormal SUMOylation might affect proteins like tau which can lead to the progression of conditions such as Alzheimer's disease. The role of UBE2I in these disorders highlights it as a potential therapeutic target in addressing these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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11 product images

Immunoprecipitation - Anti-UBE2I / UBC9 antibody [EP2938Y] (ab75854)
UBE2I / UBC9 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to UBE2I / UBC9 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70^oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab75854.
Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
Band: 18kDa: UBE2I / UBC9; non specific - 60kDa: We are unsure as to the identity of this extra band.
All lanes: Immunoprecipitation - Anti-UBE2I / UBC9 antibody [EP2938Y] (ab75854)
Predicted band size: 18 kDa
Western blot - Anti-UBE2I / UBC9 antibody [EP2938Y] (ab75854)
All lanes: Western blot - Anti-UBE2I / UBC9 antibody [EP2938Y] (ab75854) at 1/10000 dilution
Lane 1: U937 cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: Jurkat cell lysate at 10 µg
Lane 4: HUVEC cell lysate at 10 µg
Secondary
All lanes: Goat anti-rabbit HRP at 1/1000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2I / UBC9 antibody [EP2938Y] (ab75854)
ab75854, at 1/100 dilution, staining UBE2I / UBC9 in human brain, by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-UBE2I / UBC9 antibody [EP2938Y] (ab75854)
Immunocytochemistry/ Immunofluorescence analysis of Jurkat (human T cell leukemia T lymphocyte) cells labeling UBE2I / UBC9 with purified ab75854 at 1/100 dilution (10 μg/mL). Cells were fixed in 100% Methanol. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL) was used as the secondary antibody only control.
Flow Cytometry (Intracellular) - Anti-UBE2I / UBC9 antibody [EP2938Y] (ab75854)
Overlay histogram showing HepG2 cells stained with ab75854 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75854, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
Western blot - Anti-UBE2I / UBC9 antibody [EP2938Y] (ab75854)
Diluting and blocking buffer: 5% NFDM/TBST 5% NFDM/TBST
Low expression control: mouse skeletal muscle, rat parathyroid. rat skeletal muscle.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
All lanes: Western blot - Anti-UBE2I / UBC9 antibody [EP2938Y] (ab75854) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse spleen tissue lysate at 20 µg
Lane 3: Mouse skeletal muscle tissue lysate at 20 µg
Lane 4: Rat brain tissue lysate at 20 µg
Lane 5: Rat spleen tissue lysate at 20 µg
Lane 6: Rat parathyroid tissue lysate at 20 µg
Lane 7: Rat skeletal muscle tissue lysate at 20 µg
Lane 8: U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 3s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2I / UBC9 antibody [EP2938Y] (ab75854)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum labelling UBE2I / UBC9 with ab75854 at 1/2000 (0.227 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Nuclear staining on the Rat cerebrum

The section was incubated with ab75854 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2I / UBC9 antibody [EP2938Y] (ab75854)
Immunohistochemical analysis of paraffin-embedded Mouse skeletal mucle with ab75854 at 1/2000 (0.227 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: Negligible staining on Mouse skeletal muscle.
The section was incubated with aab75854 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Counterstained with Hematoxylin.
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2I / UBC9 antibody [EP2938Y] (ab75854)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum labelling UBE2I / UBC9 with ab75854 at 1/2000 (0.227 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Nuclear staining on the mouse cerebrum

The section was incubated with ab75854 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2I / UBC9 antibody [EP2938Y] (ab75854)
Immunohistochemical analysis of paraffin-embedded Rat skeletal mucle with ab75854 at 1/2000 (0.227 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: Negligible staining on Rat skeletal muscle.
The section was incubated with aab75854 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Counterstained with Hematoxylin.
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2I / UBC9 antibody [EP2938Y] (ab75854)
Immunohistochemical analysis of paraffin-embedded Rat spleen labelling UBE2I / UBC9 with ab75854 at 1/2000 (0.227 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on the Rat spleen

The section was incubated with ab75854 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.