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AB106097

Anti-UBE2J2 antibody

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(1 Publication)

Rabbit Polyclonal UBE2J2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human UBE2J2.

View Alternative Names

NCUBE2, UBE2J2, Ubiquitin-conjugating enzyme E2 J2, E2 ubiquitin-conjugating enzyme J2, Non-canonical ubiquitin-conjugating enzyme 2, NCUBE-2

1 Images
Western blot - Anti-UBE2J2 antibody (AB106097)
  • WB

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Western blot - Anti-UBE2J2 antibody (AB106097)

All lanes:

Western blot - Anti-UBE2J2 antibody (ab106097) at 1/500 dilution

Lane 1:

293 cells over-expressing Myc-UBE2J2

Lane 2:

lysate from mock-transfected 293 cells

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human UBE2J2. The exact immunogen used to generate this antibody is proprietary information.

Q8N2K1

Specificity

A BLAST analysis was used to suggest cross-reactivity with Ube2j2 protein from human, horse, opossum, cattle, dog, mouse, rat, macaque, salmon, chicken, zebra finch, Xenopus and platypus based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Opossum": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebra finch": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
sterile filtered
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBE2J2 also known as UBC6e is an E2 ubiquitin-conjugating enzyme with a molecular mass of around 35 kDa. It is involved in the ubiquitination process transferring ubiquitin from an E1 activating enzyme to a substrate often marking the substrate for degradation by the proteasome. UBE2J2 localizes primarily in the endoplasmic reticulum (ER) membrane but you can also find it distributed in other cellular membranes.
Biological function summary

The ubiquitin-conjugating enzyme UBE2J2 plays a role in protein quality control helping in the ER-associated degradation (ERAD) pathway. This process is important for maintaining cellular homeostasis by degrading misfolded or improperly assembled proteins. UBE2J2 may function in conjunction with other proteins like E3 ligases to form a complete complex required for the ubiquitination and subsequent degradation of target proteins.

Pathways

The enzyme UBE2J2 integrates with the ERAD pathway a significant component of the protein degradation system in cells. It also interacts with the ubiquitin-proteasome pathway which is important for degrading unwanted and misfolded proteins. UBE2J2 has shown interaction with several E3 ligases such as Hrd1 which facilitates the transfer of ubiquitin onto target substrates. This integration ensures the quality control of proteins within the ER.

Mutations or dysregulations in UBE2J2 can contribute to neurodegenerative diseases like Parkinson's disease due to its role in protein degradation. The impaired function and accumulation of misfolded proteins can exacerbate these conditions. Additionally cancer research has shown that overexpression of UBE2J2 might be linked to tumor progression such as in hepatocellular carcinoma. Its connection with proteins like p53 is critical as p53 is known to be a tumor suppressor and its regulation can be influenced by UBE2J2 activity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD) (By similarity). In cooperation with the GATOR2 complex, catalyzes 'Lys-6'-linked ubiquitination of NPRL2 (PubMed : 36528027).
See full target information UBE2J2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e22336 PubMed35522243

2022

Canonical and noncanonical regulatory roles for JAK2 in the pathogenesis of rheumatoid arthritis-associated interstitial lung disease and idiopathic pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Shaohua Wang,Mengfei Liu,Xiujuan Li,Jie Zhang,Faping Wang,Chujie Zhang,Anja Roden,Jay H Ryu,Kenneth J Warrington,Jie Sun,Eric L Matteson,Daniel J Tschumperlin,Robert Vassallo
View all publications
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Complementary Products

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