AB247719

Anti-Ube2L3/UBCH7 antibody [EPR4368] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Ube2L3/UBCH7 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

UBCE7, UBCH7, UBE2L3, Ubiquitin-conjugating enzyme E2 L3, E2 ubiquitin-conjugating enzyme L3, L-UBC, UbcH7, Ubiquitin carrier protein L3, Ubiquitin-conjugating enzyme E2-F1, Ubiquitin-protein ligase L3

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube2L3/UBCH7 antibody [EPR4368] - BSA and Azide free (AB247719)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube2L3/UBCH7 antibody [EPR4368] - BSA and Azide free (AB247719)

This data was developed using ab108936, the same antibody clone in a different buffer formulation.

ab108936, at a 1/100 dilution, staining Ube2L3/UBCH7 in paraffin-embedded Human testis tissue

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Ube2L3/UBCH7 antibody [EPR4368] - BSA and Azide free (AB247719)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Ube2L3/UBCH7 antibody [EPR4368] - BSA and Azide free (AB247719)

This data was developed using ab108936, the same antibody clone in a different buffer formulation.

ab108936, at a 1/100 dilution, staining Ube2L3/UBCH7 in HeLa cells

Unknown

Western blot - Anti-Ube2L3/UBCH7 antibody [EPR4368] - BSA and Azide free (AB247719)

This data was developed using ab108936, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Ube2L3/UBCH7 antibody [EPR4368] (<a href='/en-us/products/primary-antibodies/ube2l3-ubch7-antibody-epr4368-ab108936'>ab108936</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

LNCaP cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Predicted band size: 18 kDa

false

Unconjugated

Anti-Ube2L3/UBCH7 antibody [EPR4368]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4368

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab247719 is the carrier-free version of ab108936.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ube2L3 also known as UBCH7 is an E2 ubiquitin-conjugating enzyme with a molecular weight of approximately 21 kDa. This enzyme participates mechanically in the ubiquitination process where it transfers ubiquitin from an E1 activating enzyme to an E3 ligase mediating protein degradation through the proteasome. Ube2L3 is expressed ubiquitously in various tissues showing higher abundance in the brain heart and skeletal muscle.

Biological function summary

Ube2L3 plays essential roles in protein turnover and cell cycle regulation by functioning within multi-protein complexes. It helps facilitate the attachment of ubiquitin to specific target proteins marking them for degradation. These activities are important for maintaining protein homeostasis and regulation of cell division apoptosis and signal transduction. Ube2L3's role in these biological processes makes it an important component of cellular function and physiology.

Pathways

Ube2L3 is involved in the ubiquitin-proteasome pathway and is also associated with the NF-kB signaling pathway. Its function in these pathways highlights its role in regulating immune responses and inflammatory processes. Interaction with other proteins like E3 ligases including Parkin and Mdm2 shows its involvement in protein quality control and cellular stress responses. Ube2L3's modulation of these pathways is significant for proper cellular responses to environmental cues and stressors.

Ube2L3 associates with conditions like autoimmune diseases and certain cancers. Research links dysfunction in Ube2L3's activity to systemic lupus erythematosus (SLE) where immune regulation is disrupted. Furthermore studies indicate that alterations in its protein interactions particularly with E3 ligases such as Mdm2 might contribute to tumorigenesis by affecting p53 regulation. Understanding these connections helps in elucidating the roles of Ube2L3 in disease mechanisms and may support the development of targeted therapies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine; in contrast, it has activity with the RBR family E3 enzymes, such as PRKN, RNF31 and ARIH1, that function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Mediates ubiquitination by the CUL9-RBX1 complex (PubMed : 38605244). In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.

See full target information UBE2L3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 26:409 PubMed37600337

2023

Renal epithelioid angiomyolipoma: A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyun Li,Xuebei Zhang,Qingli Zhao,Jin Wang,Xiaoqing Yang,Shengliang Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ube2L3/UBCH7 antibody [EPR4368] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube2L3/UBCH7 antibody [EPR4368] - BSA and Azide free (AB247719)

Immunocytochemistry/ Immunofluorescence - Anti-Ube2L3/UBCH7 antibody [EPR4368] - BSA and Azide free (AB247719)

Western blot - Anti-Ube2L3/UBCH7 antibody [EPR4368] - BSA and Azide free (AB247719)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise