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AB109086

Anti-Ube2L6 antibody [EPR4267(2)]

3

(1 Review)

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(5 Publications)

Rabbit Recombinant Monoclonal Ube2L6 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

UBCH8, UBE2L6, Ubiquitin/ISG15-conjugating enzyme E2 L6, E2 ubiquitin-conjugating enzyme L6, Retinoic acid-induced gene B protein, UbcH8, Ubiquitin carrier protein L6, Ubiquitin-protein ligase L6, RIG-B

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube2L6 antibody [EPR4267(2)] (AB109086)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube2L6 antibody [EPR4267(2)] (AB109086)

ab109086 at 1/250 dilution staining Ube2L6 in Human kidney by Immunohistochemistry Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube2L6 antibody [EPR4267(2)] (AB109086)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube2L6 antibody [EPR4267(2)] (AB109086)

ab109086 at 1/250 dilution staining Ube2L6 in Human uterus by Immunohistochemistry Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Ube2L6 antibody [EPR4267(2)] (AB109086)
  • WB

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Western blot - Anti-Ube2L6 antibody [EPR4267(2)] (AB109086)

All lanes:

Western blot - Anti-Ube2L6 antibody [EPR4267(2)] (ab109086) at 1/1000 dilution

Lane 1:

Human fetal thymus tissue lysate at 10 µg

Lane 2:

Human lymph node tissue lysate at 10 µg

Lane 3:

Daudi cell lysate at 10 µg

Lane 4:

MOLT4 cell lysate at 10 µg

Predicted band size: 18 kDa

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  • Carrier free

    Anti-Ube2L6 antibody [EPR4267(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4267(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p>Antigen retrieval is recommended.</p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ube2L6 also known as UbcH8 is an E2 ubiquitin-conjugating enzyme with a molecular weight of about 19 kDa. It functions in the ubiquitin-proteasome pathway by transferring ubiquitin from an E1 activating enzyme to an E3 ligase assisting in protein degradation. Ube2L6 is expressed in many tissues including spleen liver and lymph nodes highlighting its varied role in cellular processes.
Biological function summary

Ube2L6 contributes to protein quality control within the cell. The enzyme participates in the post-translational modification of proteins specifically tagging misfolded or damaged proteins with ubiquitin for degradation. It interacts with several E3 ligases and forms part of the larger ubiquitin-proteasome complex. This function helps maintain protein homeostasis.

Pathways

Ube2L6 plays a role in both the NF-kB signaling and type I interferon pathways. In the NF-kB signaling pathway it facilitates the degradation of inhibitory proteins allowing nuclear translocation of NF-kB and subsequent gene expression. It associates with proteins such as TRAF6 which is important for the activation of NF-kB. In type I interferon signaling Ube2L6 aids in the antiviral response by assisting in the modification of target proteins enhancing the immune defense.

Ube2L6 relates to cancer and autoimmune conditions. In cancer its correlation with TRAF6 can influence tumor cell survival and proliferation through the NF-kB pathway. Regarding autoimmune conditions aberrant function of Ube2L6 might affect immune system responses contributing to diseases like lupus. Its regulation becomes important for maintaining normal cellular functions and preventing disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Promotes ubiquitination and subsequent proteasomal degradation of FLT3.
See full target information UBE2L6
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:17913 PubMed40410283

2025

Spermidine reduces ISGylation and enhances ISG15-USP18 interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Haruka Etori,Riko Asoshina,Takayuki Obita,Fumihiko Okumura

Oxidative medicine and cellular longevity 2022:3124122 PubMed36567857

2022

Systematic Analysis of Molecular Subtypes Based on the Expression Profile of Immune-Related Genes in Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mujing Ke

Biochemical and biophysical research communications 625:94-101 PubMed35952613

2022

Curcumin partly prevents ISG15 activation via ubiquitin-activating enzyme E1-like protein and decreases ISGylation.

Applications

Unspecified application

Species

Unspecified reactive species

Nodoka Oki,Shino Yamada,Tamaki Tanaka,Hiromi Fukui,Shigetsugu Hatakeyama,Fumihiko Okumura

Cell cycle (Georgetown, Tex.) 17:300-308 PubMed29157076

2018

UHRF1 epigenetically down-regulates UbcH8 to inhibit apoptosis in cervical cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qishu Zhang,Lijun Qiao,Xiao Wang,Changkuan Ding,Jason J Chen

Journal of virology 91: PubMed28724761

2017

ISG15 Modulates Type I Interferon Signaling and the Antiviral Response during Hepatitis E Virus Replication.

Applications

Unspecified application

Species

Unspecified reactive species

Harini Sooryanarain,Adam J Rogers,Dianjun Cao,Mary Etna R Haac,Yogesh A Karpe,Xiang-Jin Meng
View all publications
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Product promise

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