AB124946

Anti-UBE2M/UBC12 antibody [EPR5334]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal UBE2M/UBC12 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

UBC12, UBE2M, NEDD8-conjugating enzyme Ubc12, NEDD8 carrier protein, Ubiquitin-conjugating enzyme E2 M

1 Images

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Western blot - Anti-UBE2M/UBC12 antibody [EPR5334] (AB124946)

All lanes:

Western blot - Anti-UBE2M/UBC12 antibody [EPR5334] (ab124946) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

Ramos cell lysate at 10 µg

Secondary

All lanes:

standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5334

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBE2M also called UBC12 is an E2 enzyme involved in the process of neddylation which is a type of ubiquitin-like modification. The protein has a molecular mass of approximately 22 kDa. It activates and transfers NEDD8 a ubiquitin-like protein to specific substrates. UBE2M is expressed in various tissues with high expression levels in the liver muscle and kidney tissues. Its precise mechanical role is connecting to NEDD8 and collaborating with E3 ligases to modify substrates.

Biological function summary

E2 conjugating enzyme such as UBE2M functions in the regulation of the cell cycle signal transduction and other cellular processes. It is important in the neddylation pathway where it facilitates the covalent attachment of the NEDD8 molecule to Cullin proteins. UBE2M associates with the cullin-RING ligase complex assisting in the alteration of protein stability and function within cells. The proper operation of this enzyme is essential for maintaining cellular homeostasis.

Pathways

Neddylation modulates ubiquitination and acts as a regulator in protein degradation pathways. UBE2M occupies a central role in the neddylation pathway which intersects with the ubiquitin-proteasome system involving proteins like Cullin and NEDD8. Both neddylation and ubiquitination pathways share overlapping components and functions making UBE2M critical in selective protein degradation impacting processes such as DNA replication and repair and response to stress.

The incorrect function of UBE2M has links to cancer and neurodegenerative diseases. In cancer misregulation of the neddylation pathway can lead to unregulated cell growth and survival due to the aberrant degradation of key proteins. Abnormal UBE2M activity impacts the regulation of the tumor suppressor protein p53 through interaction with other neddylation-related proteins affecting cell cycle control. In neurodegenerative diseases alterations in protein homeostasis and aggregation occur related to the dysregulation of neddylation influencing proteins involved in neuronal function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.

See full target information UBE2M

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

European journal of medical research 30:859 PubMed41013641

2025

RHBDD1 induces cell cycle arrest in TP53-mutant NSCLC cells by promoting endoplasmic reticulum-associated degradation of p53 and DNA-PKcs.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Chen,Yang Wang,Shuai Shen,Libin Zhang,Jun Liu,Xiangyun Yan,Hao Peng,Zheyuan Xu

Biochemical and biophysical research communication 529:970-976 PubMed32819607

2020

Ubiquitin conjugating enzyme E2 M promotes apoptosis in osteoarthritis chondrocytes via Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Ba,Xiaohui Ni,Junlong Yu,Guoyou Zou,Hao Zhu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UBE2M/UBC12 antibody [EPR5334]

Western blot - Anti-UBE2M/UBC12 antibody [EPR5334] (AB124946)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise