AB25885

Anti-Ube2N / Ubc13 antibody

Name: Anti-Ube2N / Ubc13 antibody (ab25885) | Abcam
Brand: Abcam Limited
SKU: AB25885
Price: 545 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(11 Publications)

Rabbit Polyclonal Ube2N / Ubc13 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 11 publications.

View Alternative Names

BLU, UBE2N, Ubiquitin-conjugating enzyme E2 N, Bendless-like ubiquitin-conjugating enzyme, E2 ubiquitin-conjugating enzyme N, Ubc13, UbcH13, Ubiquitin carrier protein N, Ubiquitin-protein ligase N

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Ube2N / Ubc13 antibody (AB25885)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Ube2N / Ubc13 antibody (AB25885)

ICC/IF image of ab25885 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25885, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Western blot - Anti-Ube2N / Ubc13 antibody (AB25885)

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Western blot - Anti-Ube2N / Ubc13 antibody (ab25885) at 0.5 µg/mL

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Western blot - Anti-Ube2N / Ubc13 antibody (ab25885) at 1 µg/mL

Lane 3:

Western blot - Anti-Ube2N / Ubc13 antibody (ab25885) at 2 µg/mL

All lanes:

Human small intestine cell lysates at 15 µg

Predicted band size: 17 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

This antibody was purified by Ion exchange chromatography.

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ube2N also known as Ubc13 is a ubiquitin-conjugating enzyme with a molecular mass of around 17 kDa. It plays an important role in the ubiquitination process by forming a complex with Uev1A which assists in catalyzing the attachment of ubiquitin to substrate proteins. Expression of Ube2N is found in various tissues particularly in those with high rates of protein modification and signal regulation. Mechanically Ube2N works by forming polyubiquitin chains linked through lysine 63 on ubiquitin molecules a step essential for signaling rather than protein degradation.

Biological function summary

Ube2N has a significant role in DNA repair and immune response. It teams up with other proteins as part of larger complexes like the E2/E3 ligase complexes facilitating non-degradative polyubiquitin chains that activate signaling pathways. These chains contribute to the repair of DNA double-strand breaks by involving in the activation of DNA repair proteins and maintaining genomic integrity. Furthermore Ube2N is pivotal in modulating the immune response by activating NF-kB a transcription factor important for immune and inflammatory responses.

Pathways

Ube2N's activities impact the important signaling pathways such as NF-kB and DNA damage response pathways. In the NF-kB pathway K63-linked polyubiquitination by Ube2N leads to the activation of IKK complex subsequently mobilizing NF-kB to the nucleus to initiate transcription of immune genes. In DNA damage response Ube2N interacts with proteins like Ubc13 and Rnf8 to enhance DNA repair processes. These interactions highlight Ube2N's connection within a network of proteins maintaining cell health.

Ube2N is linked to cancer progression and immune system dysfunctions. Abnormal activity or expression of Ube2N can lead to unchecked cellular proliferation seen in various types of cancer. Additionally Ube2N associates with immune disorders where deregulated NF-kB activity contributes to chronic inflammation and auto-immune conditions. Its interaction partners like Uev1A can also play a role in the dysregulated pathways contributing to these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. Together with RNF135 and UB2V1, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIGI to activate the downstream signaling pathway that leads to interferon beta production (PubMed : 28469175, PubMed : 31006531). UBE2V1-UBE2N together with TRAF3IP2 E3 ubiquitin ligase mediate 'Lys-63'-linked polyubiquitination of TRAF6, a component of IL17A-mediated signaling pathway.

See full target information UBE2N

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Nature 642:201-211 PubMed40269158

2025

Microbial metabolite drives ageing-related clonal haematopoiesis via ALPK1.

Applications

Unspecified application

Species

Unspecified reactive species

Puneet Agarwal,Avery Sampson,Kathleen Hueneman,Kwangmin Choi,Niels Asger Jakobsen,Emma Uible,Chiharu Ishikawa,Jennifer Yeung,Lyndsey Bolanos,Xueheng Zhao,Kenneth D Setchell,David B Haslam,Jessica Galloway-Pena,John C Byrd,Paresh Vyas,Daniel T Starczynowski

Science advances 11:eadr1938 PubMed40020067

2025

A long-lived pool of PINK1 imparts a molecular memory of depolarization-induced activity.

Applications

Unspecified application

Species

Unspecified reactive species

Liam Pollock,Ioanna Ch Georgiou,Emma V Rusilowicz-Jones,Michael J Clague,Sylvie Urbé

Nucleic acids research 52:8861-8879 PubMed38943334

2024

A RAD18-UBC13-PALB2-RNF168 axis mediates replication fork recovery in BRCA1-deficient cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Emily Cybulla,Sierra Wallace,Alice Meroni,Jessica Jackson,Sumedha Agashe,Mithila Tennakoon,Mangsi Limbu,Annabel Quinet,Elena Lomonosova,Hollie Noia,Stephanie Tirman,Matthew Wood,Delphine Lemacon,Katherine Fuh,Lee Zou,Alessandro Vindigni

Biology direct 19:35 PubMed38715121

2024

UBE2N promotes cell viability and glycolysis by promoting Axin1 ubiquitination in prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Yang,Weihua Chen,Tianyi Tao,Jun Zhang,Dehui Kong,Jidong Hao,Chao Yu,Guoqiang Liao,Hua Gong

Respiratory research 24:165 PubMed37344798

2023

CircItgb5 promotes synthetic phenotype of pulmonary artery smooth muscle cells via interacting with miR-96-5p and Uba1 in monocrotaline-induced pulmonary arterial hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Su,Huiqi Zhu,Sihao Wang,Yeping Li,Chao Yan,Jiaoyan Wang,Kejing Ying

Clinical epigenetics 15:80 PubMed37161535

2023

CRISPR-Cas9 knockout screen identifies novel treatment targets in childhood high-grade glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Wenger,Ida Karlsson,Teresia Kling,Helena Carén

iScience 12:318-332 PubMed30739015

2019

Deacetylation Inhibition Reverses PABPN1-Dependent Muscle Wasting.

Applications

Unspecified application

Species

Unspecified reactive species

Cyriel S Olie,Muhammad Riaz,Rebecca Konietzny,Philip D Charles,Adan Pinto-Fernandez,Szymon M Kiełbasa,A Aartsma-Rus,Jelle J Goeman,Benedikt M Kessler,Vered Raz

Cell death & disease 9:93 PubMed29367628

2018

The inhibition of UBC13 expression and blockage of the DNMT1-CHFR-Aurora A pathway contribute to paclitaxel resistance in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Zhang,Yan Feng,Xin-Yu Wang,Ya-Nan Zhang,Chun-Nv Yuan,Song-Fa Zhang,Yuan-Ming Shen,Yun-Feng Fu,Cai-Yun Zhou,Xiao Li,Xiao-Dong Cheng,Wei-Guo Lu,Xing Xie

Scientific reports 6:18940 PubMed26743172

2016

A Generic Platform for Cellular Screening Against Ubiquitin Ligases.

Applications

Unspecified application

Species

Unspecified reactive species

Timurs Maculins,Nikki Carter,Thierry Dorval,Kevin Hudson,J Willem M Nissink,Ronald T Hay,Husam Alwan

Cancer research 72:4974-83 PubMed22865450

2012

RNF8 regulates assembly of RAD51 at DNA double-strand breaks in the absence of BRCA1 and 53BP1.

Applications

Unspecified application

Species

Unspecified reactive species

Shinichiro Nakada,Rikako Miyamoto Yonamine,Koichi Matsuo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ube2N / Ubc13 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Ube2N / Ubc13 antibody (AB25885)

Western blot - Anti-Ube2N / Ubc13 antibody (AB25885)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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