Anti-Ube2N / Ubc13 antibody [EPR5162] - BSA and Azide free

Supplementary info

Activity summary

Ube2N also known as Ubc13 is a ubiquitin-conjugating enzyme with a molecular mass of around 17 kDa. It plays an important role in the ubiquitination process by forming a complex with Uev1A which assists in catalyzing the attachment of ubiquitin to substrate proteins. Expression of Ube2N is found in various tissues particularly in those with high rates of protein modification and signal regulation. Mechanically Ube2N works by forming polyubiquitin chains linked through lysine 63 on ubiquitin molecules a step essential for signaling rather than protein degradation.

Biological function summary

Ube2N has a significant role in DNA repair and immune response. It teams up with other proteins as part of larger complexes like the E2/E3 ligase complexes facilitating non-degradative polyubiquitin chains that activate signaling pathways. These chains contribute to the repair of DNA double-strand breaks by involving in the activation of DNA repair proteins and maintaining genomic integrity. Furthermore Ube2N is pivotal in modulating the immune response by activating NF-kB a transcription factor important for immune and inflammatory responses.

Pathways

Ube2N's activities impact the important signaling pathways such as NF-kB and DNA damage response pathways. In the NF-kB pathway K63-linked polyubiquitination by Ube2N leads to the activation of IKK complex subsequently mobilizing NF-kB to the nucleus to initiate transcription of immune genes. In DNA damage response Ube2N interacts with proteins like Ubc13 and Rnf8 to enhance DNA repair processes. These interactions highlight Ube2N's connection within a network of proteins maintaining cell health.

Associated diseases and disorders

Ube2N is linked to cancer progression and immune system dysfunctions. Abnormal activity or expression of Ube2N can lead to unchecked cellular proliferation seen in various types of cancer. Additionally Ube2N associates with immune disorders where deregulated NF-kB activity contributes to chronic inflammation and auto-immune conditions. Its interaction partners like Uev1A can also play a role in the dysregulated pathways contributing to these diseases.