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AB249105

Anti-UBE2Q2 antibody [EPR10229] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal UBE2Q2 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Ubiquitin-conjugating enzyme E2 Q2, E2 ubiquitin-conjugating enzyme Q2, Ubiquitin carrier protein Q2, Ubiquitin-protein ligase Q2, UBE2Q2

1 Images
Western blot - Anti-UBE2Q2 antibody [EPR10229] - BSA and Azide free (AB249105)
  • WB

Unknown

Western blot - Anti-UBE2Q2 antibody [EPR10229] - BSA and Azide free (AB249105)

This data was developed using ab154797, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-UBE2Q2 antibody [EPR10229] (<a href='/en-us/products/primary-antibodies/ube2q2-antibody-epr10229-ab154797'>ab154797</a>) at 1/1000 dilution

Lane 1:

U87 MG lysate at 10 µg

Lane 2:

fetal brain lysate at 10 µg

Lane 3:

HeLa lysate at 10 µg

Lane 4:

293T lysate at 10 µg

Predicted band size: 43 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10229

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab249105 is the carrier-free version of ab154797.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBE2Q2 also known as Ubiquitin-Conjugating Enzyme E2 Q2 is a member of the E2 ubiquitin-conjugating enzyme family with a molecular mass of approximately 48 kDa. It plays a mechanical role in the ubiquitination process by transferring activated ubiquitin from E1-carrying enzymes to target substrates tagging them for proteasomal degradation. This protein is widely expressed in various tissues including those in the brain liver and lungs.
Biological function summary

UBE2Q2 contributes to protein homeostasis by participating in the ubiquitin-proteasome pathway which maintains protein turnover within cells. It associates with E3 ligase complexes to select substrate specificity making it essential for regulating proteins related to cell cycle and growth. Research indicates its involvement in managing regulatory proteins that control cell division and apoptosis indicating its influence on cellular maintenance and stability.

Pathways

Cellular growth and apoptosis processes involve UBE2Q2 in ubiquitin-mediated protein degradation pathways. It often collaborates with other ubiquitin-conjugating enzymes and E3 ligases such as CBL and NEDD4 focusing on protein quality control. The Wnt signaling pathway which is important for cell proliferation and differentiation also receives input from UBE2Q2 through its regulatory actions on beta-catenin turnover.

UBE2Q2 has connections to cancer development particularly colorectal and breast cancers. Aberrant expression or dysfunction of UBE2Q2 can lead to disrupted proteostasis and altered cellular signaling contributing to tumorigenesis. The oncogene CBL which interacts with UBE2Q2 is also implicated in these cancer types further highlighting UBE2Q2's role in cancer pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination.
See full target information UBE2Q2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 60:2744-2760.e9 PubMed40460835

2025

ATG9A and ARFIP2 cooperate to control PI4P levels for lysosomal repair.

Applications

Unspecified application

Species

Unspecified reactive species

Stefano De Tito,Eugenia Almacellas,Daniel Dai Yu,Emily Millard,Wenxin Zhang,Cecilia de Heus,Christophe Queval,Javier H Hervás,Enrica Pellegrino,Ioanna Panagi,Ditte Fogde,Teresa L M Thurston,Judith Klumperman,Maximiliano Gutierrez,Sharon A Tooze
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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