AB177508

Anti-UBE2S antibody

Name: Anti-UBE2S antibody (ab177508) | Abcam
Brand: Abcam Limited
SKU: AB177508
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal UBE2S antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human UBE2S aa 150 to C-terminus.

View Alternative Names

E2EPF, OK/SW-cl.73, UBE2S, Ubiquitin-conjugating enzyme E2 S, E2 ubiquitin-conjugating enzyme S, E2-EPF, Ubiquitin carrier protein S, Ubiquitin-conjugating enzyme E2-24 kDa, Ubiquitin-conjugating enzyme E2-EPF5, Ubiquitin-protein ligase S

2 Images

Supplier Data

Immunoprecipitation - Anti-UBE2S antibody (AB177508)

Detection of UBE2S in Immunoprecipitates of 293Twhole cell lysates (1 mg for IP, 20% of IP loaded) using ab177508 at 6 μg/mg lysate for IP (Lane 1). For WB detection, ab177508 was used at 1 μg/ml. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-UBE2S antibody (ab177508)

Predicted band size: 24 kDa

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Supplier Data

Western blot - Anti-UBE2S antibody (AB177508)

All lanes:

Western blot - Anti-UBE2S antibody (ab177508) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

mouse TCMK1 whole cell lysate at 50 µg

Lane 5:

mouse NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 24 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human UBE2S aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q16763

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab177508 was affinity purified using an epitope specific to UBE2S immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBE2S also known as Ubiquitin-conjugating enzyme E2 S is an important player in the ubiquitin-proteasome system. This enzyme has a mass of approximately 22 kDa. UBE2S is highly expressed in tissues with rapid proliferation such as testes and certain types of tumors. Its main function involves catalyzing the elongation of ubiquitin chains specifically on lysine 11 a modification that predominantly signals for cell cycle-related proteolysis.

Biological function summary

UBE2S acts within the anaphase-promoting complex/cyclosome (APC/C) an important multi-subunit E3 ubiquitin ligase. Through its activity UBE2S facilitates the progression of the mitotic cycle by tagging key regulatory proteins for degradation aiding in their timely removal during cell division. Without this regulation cells struggle with correct chromosomal segregation which can lead to genomic instability.

Pathways

UBE2S functions notably within the cell cycle regulation and protein degradation pathways. It collaborates closely with the anaphase-promoting complex/cyclosome (APC/C) and cooperates with related proteins such as CDC20 and CDH1. By enabling the degradation of securin and cyclins UBE2S helps manage the transition from metaphase to anaphase ensuring cells divide properly in mitotic processes.

UBE2S has strong implications in cancer development particularly in breast and lung cancers. Overexpression of UBE2S often correlates with tumor progression and poor prognosis due to its role in promoting uncontrolled cell division. Additionally linkages between UBE2S and proteins like cyclin B1 suggest disruptions might contribute to tumor cell survival providing potential therapeutic targets in oncology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed : 19820702, PubMed : 19822757, PubMed : 22496338, PubMed : 27259151). Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis (PubMed : 19820702, PubMed : 19822757, PubMed : 27259151, PubMed : 27910872). Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit (PubMed : 19820702, PubMed : 19822757, PubMed : 27259151). Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A (PubMed : 16819549). In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination (PubMed : 20061386, PubMed : 20622874).

See full target information UBE2S

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of oncology 2022:3982539 PubMed35578600

2022

A Pan-Cancer Analysis of UBE2S in Tumorigenesis, Prognosis, Pathway, Immune Infiltration and Evasion, and Therapy Response from an Immune-Oncology Perspective.

Applications

Unspecified application

Species

Unspecified reactive species

Haili Bao,Yi Luo,Guoshan Ding,Zhiren Fu

Cell death discovery 7:357 PubMed34785642

2021

UBE2S promotes cell chemoresistance through PTEN-AKT signaling in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Gui,Sicai Zhang,Yongzi Xu,Hongwei Zhang,Ying Zhu,Lianbao Kong

Cell reports 32:108102 PubMed32905768

2020

The Negative Cross-Talk between SAG/RBX2/ROC2 and APC/C E3 Ligases in Regulation of Cell Cycle Progression and Drug Resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Shizhen Zhang,Yanwen Shen,Hua Li,Chao Bi,Yilun Sun,Xiufang Xiong,Wenyi Wei,Yi Sun

Cell 171:918-933.e20 PubMed29033132

2017

Assembly and Function of Heterotypic Ubiquitin Chains in Cell-Cycle and Protein Quality Control.

Applications

Unspecified application

Species

Unspecified reactive species

Richard G Yau,Kerstin Doerner,Erick R Castellanos,Diane L Haakonsen,Achim Werner,Nan Wang,X William Yang,Nadia Martinez-Martin,Marissa L Matsumoto,Vishva M Dixit,Michael Rape

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UBE2S antibody

Immunoprecipitation - Anti-UBE2S antibody (AB177508)

Western blot - Anti-UBE2S antibody (AB177508)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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