Rabbit Polyclonal UBE2S antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human UBE2S aa 150 to C-terminus.
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Publications
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- Journal of oncology 2022:39825392022A Pan-Cancer Analysis of UBE2S in Tumorigenesis, Prognosis, Pathway, Immune Infiltration and Evasion, and Therapy Response from an Immune-Oncology Perspective.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaili Bao,Yi Luo,Guoshan Ding,Zhiren FuPubMed 35578600
- Cell death discovery 7:3572021UBE2S promotes cell chemoresistance through PTEN-AKT signaling in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Gui,Sicai Zhang,Yongzi Xu,Hongwei Zhang,Ying Zhu,Lianbao KongPubMed 34785642
- Cell reports 32:1081022020The Negative Cross-Talk between SAG/RBX2/ROC2 and APC/C E3 Ligases in Regulation of Cell Cycle Progression and Drug Resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShizhen Zhang,Yanwen Shen,Hua Li,Chao Bi,Yilun Sun,Xiufang Xiong,Wenyi Wei,Yi SunPubMed 32905768
- Cell 171:918-933.e202017Assembly and Function of Heterotypic Ubiquitin Chains in Cell-Cycle and Protein Quality Control.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRichard G Yau et. al.PubMed 29033132
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human UBE2S aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q16763
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Dog, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Dog, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
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Target data
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:19820702, PubMed:19822757, PubMed:22496338, PubMed:27259151). Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis (PubMed:19820702, PubMed:19822757, PubMed:27259151, PubMed:27910872). Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit (PubMed:19820702, PubMed:19822757, PubMed:27259151). Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A (PubMed:16819549). In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination (PubMed:20061386, PubMed:20622874).
Alternative names
E2EPF, OK/SW-cl.73, UBE2S, Ubiquitin-conjugating enzyme E2 S, E2 ubiquitin-conjugating enzyme S, E2-EPF, Ubiquitin carrier protein S, Ubiquitin-conjugating enzyme E2-24 kDa, Ubiquitin-conjugating enzyme E2-EPF5, Ubiquitin-protein ligase S
Recommended products
Rabbit Polyclonal UBE2S antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human UBE2S aa 150 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human UBE2S aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q16763
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab177508 was affinity purified using an epitope specific to UBE2S immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UBE2S also known as Ubiquitin-conjugating enzyme E2 S is an important player in the ubiquitin-proteasome system. This enzyme has a mass of approximately 22 kDa. UBE2S is highly expressed in tissues with rapid proliferation such as testes and certain types of tumors. Its main function involves catalyzing the elongation of ubiquitin chains specifically on lysine 11 a modification that predominantly signals for cell cycle-related proteolysis.
- Biological function summary
UBE2S acts within the anaphase-promoting complex/cyclosome (APC/C) an important multi-subunit E3 ubiquitin ligase. Through its activity UBE2S facilitates the progression of the mitotic cycle by tagging key regulatory proteins for degradation aiding in their timely removal during cell division. Without this regulation cells struggle with correct chromosomal segregation which can lead to genomic instability.
- Pathways
UBE2S functions notably within the cell cycle regulation and protein degradation pathways. It collaborates closely with the anaphase-promoting complex/cyclosome (APC/C) and cooperates with related proteins such as CDC20 and CDH1. By enabling the degradation of securin and cyclins UBE2S helps manage the transition from metaphase to anaphase ensuring cells divide properly in mitotic processes.
- Associated diseases and disorders
UBE2S has strong implications in cancer development particularly in breast and lung cancers. Overexpression of UBE2S often correlates with tumor progression and poor prognosis due to its role in promoting uncontrolled cell division. Additionally linkages between UBE2S and proteins like cyclin B1 suggest disruptions might contribute to tumor cell survival providing potential therapeutic targets in oncology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-UBE2S antibody (ab177508)
All lanes: Western blot - Anti-UBE2S antibody (ab177508) at 0.1 µg/mL
Lane 1: 293T whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 50 µg
Lane 3: Jurkat whole cell lysate at 50 µg
Lane 4: mouse TCMK1 whole cell lysate at 50 µg
Lane 5: mouse NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 24 kDa
Exposure time: 3min
Immunoprecipitation - Anti-UBE2S antibody (ab177508)
Detection of UBE2S in Immunoprecipitates of 293Twhole cell lysates (1 mg for IP, 20% of IP loaded) using ab177508 at 6 μg/mg lysate for IP (Lane 1). For WB detection, ab177508 was used at 1 μg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
All lanes: Immunoprecipitation - Anti-UBE2S antibody (ab177508)
Predicted band size: 24 kDa
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