AB197945

Anti-UBE2S antibody

Name: Anti-UBE2S antibody (ab197945) | Abcam
Brand: Abcam Limited
SKU: AB197945

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(2 Publications)

Rabbit Polyclonal UBE2S antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human UBE2S.

View Alternative Names

E2EPF, OK/SW-cl.73, UBE2S, Ubiquitin-conjugating enzyme E2 S, E2 ubiquitin-conjugating enzyme S, E2-EPF, Ubiquitin carrier protein S, Ubiquitin-conjugating enzyme E2-24 kDa, Ubiquitin-conjugating enzyme E2-EPF5, Ubiquitin-protein ligase S

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2S antibody (AB197945)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2S antibody (AB197945)

Immunohistochemical analysis of formalin fixed paraffin embedded human thyroid cancer labeling UBE2S with ab197945 at 1/40 dilution.

Supplier Data

Western blot - Anti-UBE2S antibody (AB197945)

8% SDS PAGE

All lanes:

Western blot - Anti-UBE2S antibody (ab197945) at 1/300 dilution

All lanes:

Human testis tissue at 40 µg

Predicted band size: 24 kDa

false

Exposure time: 2min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human UBE2S.

Q16763

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/40 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBE2S also known as Ubiquitin-conjugating enzyme E2 S is an important player in the ubiquitin-proteasome system. This enzyme has a mass of approximately 22 kDa. UBE2S is highly expressed in tissues with rapid proliferation such as testes and certain types of tumors. Its main function involves catalyzing the elongation of ubiquitin chains specifically on lysine 11 a modification that predominantly signals for cell cycle-related proteolysis.

Biological function summary

UBE2S acts within the anaphase-promoting complex/cyclosome (APC/C) an important multi-subunit E3 ubiquitin ligase. Through its activity UBE2S facilitates the progression of the mitotic cycle by tagging key regulatory proteins for degradation aiding in their timely removal during cell division. Without this regulation cells struggle with correct chromosomal segregation which can lead to genomic instability.

Pathways

UBE2S functions notably within the cell cycle regulation and protein degradation pathways. It collaborates closely with the anaphase-promoting complex/cyclosome (APC/C) and cooperates with related proteins such as CDC20 and CDH1. By enabling the degradation of securin and cyclins UBE2S helps manage the transition from metaphase to anaphase ensuring cells divide properly in mitotic processes.

UBE2S has strong implications in cancer development particularly in breast and lung cancers. Overexpression of UBE2S often correlates with tumor progression and poor prognosis due to its role in promoting uncontrolled cell division. Additionally linkages between UBE2S and proteins like cyclin B1 suggest disruptions might contribute to tumor cell survival providing potential therapeutic targets in oncology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed : 19820702, PubMed : 19822757, PubMed : 22496338, PubMed : 27259151). Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis (PubMed : 19820702, PubMed : 19822757, PubMed : 27259151, PubMed : 27910872). Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit (PubMed : 19820702, PubMed : 19822757, PubMed : 27259151). Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A (PubMed : 16819549). In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination (PubMed : 20061386, PubMed : 20622874).

See full target information UBE2S

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer medicine 12:18078-18097 PubMed37563971

2023

UBE2S promotes malignant properties via VHL/HIF-1α and VHL/JAK2/STAT3 signaling pathways and decreases sensitivity to sorafenib in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Junyi Wu,Xiangjie Xu,Shasha Wu,Weiwei Shi,Guang Zhang,Yin Cao,Zhongxia Wang,Junhua Wu,Chunping Jiang

BMB reports 51:642-647 PubMed30545437

2018

UBE2S promotes the proliferation and survival of human lung adenocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Liu,Lijun Xu

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-UBE2S antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2S antibody (AB197945)

Western blot - Anti-UBE2S antibody (AB197945)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise