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AB151725

Anti-UBE2V1 antibody [EPR4735(2)]

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(2 Publications)

Rabbit Recombinant Monoclonal UBE2V1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CROC1, UBE2V, UEV1, P/OKcl.19, UBE2V1, Ubiquitin-conjugating enzyme E2 variant 1, UEV-1, CROC-1, TRAF6-regulated IKK activator 1 beta Uev1A

1 Images
Western blot - Anti-UBE2V1 antibody [EPR4735(2)] (AB151725)
  • WB

Unknown

Western blot - Anti-UBE2V1 antibody [EPR4735(2)] (AB151725)

All lanes:

Western blot - Anti-UBE2V1 antibody [EPR4735(2)] (ab151725) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

HT29 cell lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Lane 4:

BxPC3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 16 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4735(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this antibody shares 93% identity with Ube2v2. Cross-reactivity with this protein has not been confirmed experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBE2V1 also known as ubiquitin-conjugating enzyme E2 variant 1 acts as an E2-like enzyme facilitating the transfer of ubiquitin molecules to substrate proteins. It is a non-canonical ubiquitin-conjugating enzyme because it lacks the active cysteine residue needed to conjugate ubiquitin directly. The molecular mass of UBE2V1 is approximately 20 kDa. UBE2V1 is highly expressed in various human tissues including the spleen liver and placenta suggesting its broad role in cellular functions.
Biological function summary

UBE2V1 collaborates with UBE2N (also known as Ubc13) to form a heterodimer c. This dimer is involved in the process of K63-linked polyubiquitination which does not target proteins for proteasomal degradation but rather plays an important role in DNA damage tolerance and immune response regulation. UBE2V1 when associated with UBE2N aids in the polyubiquitination of protein substrates allowing for their involvement in regulatory mechanisms that are essential for cellular homeostasis.

Pathways

UBE2V1 serves an important function in the NF-κB signaling pathway and DNA repair processes. In the NF-κB pathway UBE2V1 through its interaction with UBE2N contributes to the activation of TAK1 kinase which ultimately activates the transcription factor NF-κB leading to inflammatory and immune responses. In DNA repair it participates in error-free damage bypass mechanism alongside UBE2N. These pathways highlight the integration of UBE2V1 in cellular processes that manage stress and damage responses.

UBE2V1 associates with cancer and immune disorders. Its role in the NF-κB pathway links it to oncogenic processes as deregulated NF-κB signaling often corresponds with tumorigenesis. In addition abnormalities in UBE2V1’s function may lead to aberrant immune responses as its partnership with UBE2N influences inflammatory reactions. Such dysregulation can contribute to both the initiation and progression of diseases like rheumatoid arthritis and various cancers marking UBE2V1 as a potential target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has no ubiquitin ligase activity on its own. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. Together with RNF135 and UBE2N, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIGI to activate the downstream signaling pathway that leads to interferon beta production (PubMed : 31006531). UBE2V1-UBE2N together with TRAF3IP2 E3 ubiquitin ligase mediate 'Lys-63'-linked polyubiquitination of TRAF6, a component of IL17A-mediated signaling pathway.
See full target information UBE2V1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 11:1051 PubMed33311443

2020

Linc00426 accelerates lung adenocarcinoma progression by regulating miR-455-5p as a molecular sponge.

Applications

Unspecified application

Species

Unspecified reactive species

Hongli Li,Qingjie Mu,Guoxin Zhang,Zhixin Shen,Yuanyuan Zhang,Jun Bai,Liping Zhang,Dandan Zhou,Quan Zheng,Lihong Shi,Wenxia Su,Chonggao Yin,Baogang Zhang

Journal of hematology & oncology 11:95 PubMed30016968

2018

Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Tong Shen,Ling-Dong Cai,Yu-Hong Liu,Shi Li,Wen-Juan Gan,Xiu-Ming Li,Jing-Ru Wang,Peng-Da Guo,Qun Zhou,Xing-Xing Lu,Li-Na Sun,Jian-Ming Li
View all publications
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Product promise

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