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AB254700

Anti-UBE2Z antibody

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(1 Publication)

Rabbit Polyclonal UBE2Z antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human UBE2Z aa 200-350.

View Alternative Names

HOYS7, UBE2Z, Ubiquitin-conjugating enzyme E2 Z, E2 ubiquitin-conjugating enzyme Z, Uba6-specific E2 conjugating enzyme 1, Ubiquitin carrier protein Z, Ubiquitin-protein ligase Z, Use1

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2Z antibody (AB254700)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2Z antibody (AB254700)

Paraffin-embedded human testis tissue stained for UBE2Z using ab254700 at 1/200 dilution in immunohistochemical analysis.

Western blot - Anti-UBE2Z antibody (AB254700)
  • WB

Supplier Data

Western blot - Anti-UBE2Z antibody (AB254700)

All lanes:

Western blot - Anti-UBE2Z antibody (ab254700) at 0.4 µg/mL

All lanes:

A549 (human lung carcinoma cell line) whole cell lysate

Predicted band size: 38 kDa

false

Western blot - Anti-UBE2Z antibody (AB254700)
  • WB

Supplier Data

Western blot - Anti-UBE2Z antibody (AB254700)

All lanes:

Western blot - Anti-UBE2Z antibody (ab254700) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II (rat Wistar bladder tumor cell line) cell lysate

Predicted band size: 38 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-UBE2Z antibody (AB254700)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-UBE2Z antibody (AB254700)

PFA fixed, Triton X-100 permeabilized A-431 (Human epidermoid carcinoma cell line) cells labeling UBE2Z using ab254700 at 4µg/ml (green) in ICC/IF.

Western blot - Anti-UBE2Z antibody (AB254700)
  • WB

Supplier Data

Western blot - Anti-UBE2Z antibody (AB254700)

Lane 1:

Western blot - Anti-UBE2Z antibody (ab254700)

Lanes 2 - 4:

Western blot - Anti-UBE2Z antibody (ab254700) at 0.4 µg/mL

Lane 1:

DNA Ladder

Lane 2:

U-138MG cells transfected with the control siRNA

Lane 3:

U-138MG cells transfected with target-specific siRNA probe #1

Lane 4:

U-138MG cells transfected with target-specific siRNA probe #2

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human UBE2Z aa 200-350. The exact immunogen used to generate this antibody is proprietary information.

Q9H832

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBE2Z also known as Ubiquitin-Conjugating Enzyme E2 Z is an enzyme that plays a mechanical role in the ubiquitination process. This enzyme belongs to the E2 ubiquitin-conjugating enzyme family. UBE2Z has a mass of approximately 37.5 kDa. It is expressed in various tissues including but not limited to the liver heart and skeletal muscle. The enzyme catalyzes the transfer of ubiquitin from an E1 ubiquitin-activating enzyme to E3 ubiquitin ligases facilitating substrate ubiquitination.
Biological function summary

UBE2Z is involved in protein degradation and regulation of protein homeostasis. It is an important part of the ubiquitin-proteasome system ensuring damaged or misfolded proteins are marked for degradation. UBE2Z can function independently or in conjunction with multi-protein complexes. It has an important role in regulating cellular processes like cell cycle progression DNA repair and response to stress. Additionally it contributes to the regulation of several cellular pathways through these processes.

Pathways

UBE2Z is significantly involved in the protein ubiquitination pathway and cellular response to oxidative stress. In the protein ubiquitination pathway it interacts with various E3 ligases influencing protein turnover. Notably it interacts with the KEAP1 pathway which regulates the degradation of NRF2 a transcription factor involved in antioxidative responses. UBE2Z’s association with these pathways links it to the modulation of protein stability and cellular sensitivity to oxidative damage.

UBE2Z has been linked to metabolic disorders and renal diseases. Research has shown that variations in the UBE2Z gene have associations with obesity-related traits and Type 2 diabetes. The enzyme also has connections to proteins like NRF2 and KEAP1 which play roles in the development of renal pathologies. Dysregulation in UBE2Z activity can contribute to oxidative stress and inflammation further influencing disease progression in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity). Specific substrate for UBA6, not charged with ubiquitin by UBE1. May be involved in apoptosis regulation.
See full target information UBE2Z
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 23:e14320 PubMed39158018

2024

Single-cell RNA sequencing reveals the CRTAC1 population actively contributes to the pathogenesis of spinal ligament degeneration by SPP1 macrophage.

Applications

Unspecified application

Species

Unspecified reactive species

Yulong Tang,Dachun Zhuo,Yuexin Yu,Weilin Pu,Yanyun Ma,Yuting Zhang,Yan Huang,Qing Zhang,Kunhai Tang,Chen Meng,Di Yang,Lu Bai,Dongyi He,Li Jin,Hejian Zou,Huji Xu,Qi Zhu,Jiucun Wang,Yuanyuan Chen,Jing Liu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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