Rabbit Polyclonal UBE2Z antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human UBE2Z aa 200-350.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human UBE2Z aa 200-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H832
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity). Specific substrate for UBA6, not charged with ubiquitin by UBE1. May be involved in apoptosis regulation.
Alternative names
HOYS7, UBE2Z, Ubiquitin-conjugating enzyme E2 Z, E2 ubiquitin-conjugating enzyme Z, Uba6-specific E2 conjugating enzyme 1, Ubiquitin carrier protein Z, Ubiquitin-protein ligase Z, Use1
Recommended products
Rabbit Polyclonal UBE2Z antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human UBE2Z aa 200-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human UBE2Z aa 200-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H832
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UBE2Z also known as Ubiquitin-Conjugating Enzyme E2 Z is an enzyme that plays a mechanical role in the ubiquitination process. This enzyme belongs to the E2 ubiquitin-conjugating enzyme family. UBE2Z has a mass of approximately 37.5 kDa. It is expressed in various tissues including but not limited to the liver heart and skeletal muscle. The enzyme catalyzes the transfer of ubiquitin from an E1 ubiquitin-activating enzyme to E3 ubiquitin ligases facilitating substrate ubiquitination.
- Biological function summary
UBE2Z is involved in protein degradation and regulation of protein homeostasis. It is an important part of the ubiquitin-proteasome system ensuring damaged or misfolded proteins are marked for degradation. UBE2Z can function independently or in conjunction with multi-protein complexes. It has an important role in regulating cellular processes like cell cycle progression DNA repair and response to stress. Additionally it contributes to the regulation of several cellular pathways through these processes.
- Pathways
UBE2Z is significantly involved in the protein ubiquitination pathway and cellular response to oxidative stress. In the protein ubiquitination pathway it interacts with various E3 ligases influencing protein turnover. Notably it interacts with the KEAP1 pathway which regulates the degradation of NRF2 a transcription factor involved in antioxidative responses. UBE2Z’s association with these pathways links it to the modulation of protein stability and cellular sensitivity to oxidative damage.
- Associated diseases and disorders
UBE2Z has been linked to metabolic disorders and renal diseases. Research has shown that variations in the UBE2Z gene have associations with obesity-related traits and Type 2 diabetes. The enzyme also has connections to proteins like NRF2 and KEAP1 which play roles in the development of renal pathologies. Dysregulation in UBE2Z activity can contribute to oxidative stress and inflammation further influencing disease progression in these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Immunocytochemistry/ Immunofluorescence - Anti-UBE2Z antibody (ab254700)
PFA fixed, Triton X-100 permeabilized A-431 (Human epidermoid carcinoma cell line) cells labeling UBE2Z using ab254700 at 4µg/ml (green) in ICC/IF.
Western blot - Anti-UBE2Z antibody (ab254700)
Lane 1: Western blot - Anti-UBE2Z antibody (ab254700)
Lanes 2 - 4: Western blot - Anti-UBE2Z antibody (ab254700) at 0.4 µg/mL
Lane 1: DNA Ladder
Lane 2: U-138MG cells transfected with the control siRNA
Lane 3: U-138MG cells transfected with target-specific siRNA probe #1
Lane 4: U-138MG cells transfected with target-specific siRNA probe #2
Predicted band size: 38 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2Z antibody (ab254700)
Paraffin-embedded human testis tissue stained for UBE2Z using ab254700 at 1/200 dilution in immunohistochemical analysis.
Western blot - Anti-UBE2Z antibody (ab254700)
All lanes: Western blot - Anti-UBE2Z antibody (ab254700) at 0.4 µg/mL
Lane 1: NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 2: NBT-II (rat Wistar bladder tumor cell line) cell lysate
Predicted band size: 38 kDa
Western blot - Anti-UBE2Z antibody (ab254700)
All lanes: Western blot - Anti-UBE2Z antibody (ab254700) at 0.4 µg/mL
All lanes: A549 (human lung carcinoma cell line) whole cell lysate
Predicted band size: 38 kDa
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