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Rabbit Polyclonal UBE3A antibody. Suitable for IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human UBE3A aa 1-450.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3A antibody (AB183869), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3A antibody (AB183869), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human UBE3A aa 1-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q05086

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested
Mouse
Tested

Tested
Tested

Species

Mouse

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Associated Products

Select an associated product type

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Target data

Function

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates (PubMed:10373495, PubMed:16772533, PubMed:19204938, PubMed:19233847, PubMed:19325566, PubMed:19591933, PubMed:22645313, PubMed:24273172, PubMed:24728990, PubMed:30020076). Several substrates have been identified including the BMAL1, ARC, LAMTOR1, RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B (PubMed:10373495, PubMed:19204938, PubMed:19325566, PubMed:19591933, PubMed:22645313, PubMed:24728990, PubMed:30020076). Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins (PubMed:19233847). Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component BMAL1, leading to its proteasomal degradation (PubMed:24728990). Acts as transcriptional coactivator of progesterone receptor PGR upon progesterone hormone activation (PubMed:16772533). Acts as a regulator of synaptic development by mediating ubiquitination and degradation of ARC (By similarity). Required for synaptic remodeling in neurons by mediating ubiquitination and degradation of LAMTOR1, thereby limiting mTORC1 signaling and activity-dependent synaptic remodeling (By similarity). Synergizes with WBP2 in enhancing PGR activity (PubMed:16772533).(Microbial infection) Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses.

Alternative names

Recommended products

Rabbit Polyclonal UBE3A antibody. Suitable for IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human UBE3A aa 1-450.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human UBE3A aa 1-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q05086
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

UBE3A also known as ubiquitin protein ligase E3A is an enzyme with a molecular mass of approximately 100 kDa. It plays a central role in the ubiquitination process by adding ubiquitin to specific substrate proteins. UBE3A is found in many tissues but has high expression levels in the brain especially in neurons. The proper function of UBE3A requires its interaction with the ubiquitin-conjugating enzyme E2 marking target proteins for proteasomal degradation or other cellular processes.

Biological function summary

The role of UBE3A is connected to protein homeostasis and turnover. It is part of the ubiquitin-proteasome system which regulates the degradation of damaged or unneeded proteins. UBE3A does not work alone; it functions as part of a larger E3 ligase complex. This complex selectively identifies and modifies proteins determining their fate within the cell. The precision of UBE3A in these processes is necessary for normal neural development and synaptic plasticity.

Pathways

UBE3A is involved in the synaptic transmission and neuronal signaling pathways. It interacts with proteins such as HERC2 and RAD18 which are involved in DNA damage response and repair. UBE3A's involvement in these pathways highlights its importance in maintaining neuronal health. This protein’s activity influences downstream signaling events affecting processes like synaptic function and neuronal communication.

Associated diseases and disorders

Disruptions in UBE3A activity link to Angelman syndrome and autism spectrum disorders. Angelman syndrome results from the loss of function of the maternal UBE3A gene leading to severe neurological impairments. UBE3A's relationship with proteins like MECP2 involved in gene regulation suggests a broader role in neurodevelopmental disorders. Research continues to explore how UBE3A and its interaction partners contribute to the underlying mechanisms of these diseases.

Product promise

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2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3A antibody (ab183869), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3A antibody (ab183869)

    Immunohistochemical analysis of paraffin-embedded mouse middle brain tissue, labeling UBE3A using ab183869 at a 1/500 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3A antibody (ab183869), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3A antibody (ab183869)

    Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue, labeling UBE3A using ab183869 at a 1/500 dilution.

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Product protocols

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