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AB101512

Anti-UBE3C antibody

3

(1 Review)

|

(3 Publications)

Rabbit Polyclonal UBE3C antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human UBE3C aa 150-400.

View Alternative Names

KIAA0010, KIAA10, UBE3C, Ubiquitin-protein ligase E3C, HECT-type ubiquitin transferase E3C, Homologous to E6AP carboxyl terminus homologous protein 2, RTA-associated ubiquitin ligase, HectH2, RAUL

1 Images
Western blot - Anti-UBE3C antibody (AB101512)
  • WB

Unknown

Western blot - Anti-UBE3C antibody (AB101512)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-UBE3C antibody (ab101512) at 1/1000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 124 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human UBE3C aa 150-400. The exact immunogen used to generate this antibody is proprietary information.

Q15386

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBE3C also known as ubiquitin protein ligase E3C is an E3 ubiquitin-protein ligase that plays a mechanical role in tagging proteins for degradation. This tagging involves attaching ubiquitin molecules to substrate proteins which targets them for proteasomal degradation. UBE3C has a molecular mass of approximately 90 kDa. Expression of this protein occurs in various tissues with higher levels found in the brain and testis suggesting these organs may rely more on its function.
Biological function summary

UBE3C regulates protein homeostasis by working as part of the ubiquitin-proteasome system which maintains cellular function by removing damaged or unneeded proteins. It assists in controlling protein quality and stability. Although not typically part of larger multiprotein complexes UBE3C influences the turnover of several cellular proteins. Its action affects many processes including neuronal function and response to cellular stress.

Pathways

UBE3C activity intersects with the ubiquitin-proteasome pathway a fundamental mechanism for protein degradation and turnover in cells. This pathway ensures cellular protein quality and prevents toxic protein accumulation. It has associations with proteins like UCHL1 which also have roles in the ubiquitination process. Moreover UBE3C links to the Wnt signaling pathway by modulating the stability of pathway components such as β-catenin which influences cell proliferation and differentiation.

UBE3C involvement appears significant in neurodegenerative diseases and certain cancers. In neurodegenerative conditions like Alzheimer's disease altered UBE3C expression or function might impact protein aggregates formation similar to its interaction with proteins like tau. In cancer abnormal UBE3C levels relate to changes in protein degradation that can lead to unchecked cell growth and survival affecting proteins like p53 an important tumor suppressor.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that specifically catalyzes 'Lys-29'- and 'Lys-48'-linked polyubiquitin chains (PubMed : 11278995, PubMed : 12692129, PubMed : 16341092, PubMed : 16601690, PubMed : 24158444, PubMed : 24811749, PubMed : 25752573, PubMed : 25752577, PubMed : 32039437, PubMed : 33637724, PubMed : 34239127). Accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed : 32039437, PubMed : 9575161). Associates with the proteasome and promotes elongation of ubiquitin chains on substrates bound to the 26S proteasome (PubMed : 24158444, PubMed : 28396413, PubMed : 31375563). Also catalyzes 'Lys-29'- and 'Lys-48'-linked ubiquitination of 26S proteasome subunit ADRM1/RPN13 in response to proteotoxic stress, impairing the ability of the proteasome to bind and degrade ubiquitin-conjugated proteins (PubMed : 24811749, PubMed : 31375563). Acts as a negative regulator of autophagy by mediating 'Lys-29'- and 'Lys-48'-linked ubiquitination of PIK3C3/VPS34, promoting its degradation (PubMed : 33637724). Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains; with some preference for 'Lys-48' linkages (PubMed : 11278995, PubMed : 16601690, PubMed : 25752577). Acts as a negative regulator of type I interferon by mediating 'Lys-48'-linked ubiquitination of IRF3 and IRF7, leading to their degradation by the proteasome (PubMed : 21167755). Catalyzes ubiquitination and degradation of CAND2 (PubMed : 12692129).
See full target information UBE3C
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 21:25 PubMed33407510

2021

Oncogenic UBE3C promotes breast cancer progression by activating Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Hang,Shanojie Zhao,Tiejun Wang,Yan Zhang

Molecular cell 67:181-193.e5 PubMed28689657

2017

Wnt-Dependent Inactivation of the Groucho/TLE Co-repressor by the HECT E3 Ubiquitin Ligase Hyd/UBR5.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua E Flack,Juliusz Mieszczanek,Nikola Novcic,Mariann Bienz

Chembiochem : a European journal of chemical biolo 18:1415-1427 PubMed28425671

2017

Activity-Based Probes for HECT E3 Ubiquitin Ligases.

Applications

Unspecified application

Species

Unspecified reactive species

Robert Byrne,Thomas Mund,Julien D F Licchesi
View all publications
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Product promise

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