AB177511

Anti-UBE3C antibody

Name: Anti-UBE3C antibody (ab177511) | Abcam
Brand: Abcam Limited
SKU: AB177511

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(1 Publication)

Rabbit Polyclonal UBE3C antibody. Suitable for IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human UBE3C aa 700-800.

View Alternative Names

KIAA0010, KIAA10, UBE3C, Ubiquitin-protein ligase E3C, HECT-type ubiquitin transferase E3C, Homologous to E6AP carboxyl terminus homologous protein 2, RTA-associated ubiquitin ligase, HectH2, RAUL

1 Images

Supplier Data

Immunoprecipitation - Anti-UBE3C antibody (AB177511)

Detection of Human UBE3C by Western Blot of Immunoprecipitates.

Lane 1 : Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells.

Lane 2 : Control IgG.

ab177511 used for IP at 6 μg/mg lysate.

Detection : Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-UBE3C antibody (ab177511)

Predicted band size: 124 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human UBE3C aa 700-800. The exact immunogen used to generate this antibody is proprietary information.

Q15386

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab177511 was affinity purified using an epitope specific to UBE3C immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBE3C also known as ubiquitin protein ligase E3C is an E3 ubiquitin-protein ligase that plays a mechanical role in tagging proteins for degradation. This tagging involves attaching ubiquitin molecules to substrate proteins which targets them for proteasomal degradation. UBE3C has a molecular mass of approximately 90 kDa. Expression of this protein occurs in various tissues with higher levels found in the brain and testis suggesting these organs may rely more on its function.

Biological function summary

UBE3C regulates protein homeostasis by working as part of the ubiquitin-proteasome system which maintains cellular function by removing damaged or unneeded proteins. It assists in controlling protein quality and stability. Although not typically part of larger multiprotein complexes UBE3C influences the turnover of several cellular proteins. Its action affects many processes including neuronal function and response to cellular stress.

Pathways

UBE3C activity intersects with the ubiquitin-proteasome pathway a fundamental mechanism for protein degradation and turnover in cells. This pathway ensures cellular protein quality and prevents toxic protein accumulation. It has associations with proteins like UCHL1 which also have roles in the ubiquitination process. Moreover UBE3C links to the Wnt signaling pathway by modulating the stability of pathway components such as β-catenin which influences cell proliferation and differentiation.

UBE3C involvement appears significant in neurodegenerative diseases and certain cancers. In neurodegenerative conditions like Alzheimer's disease altered UBE3C expression or function might impact protein aggregates formation similar to its interaction with proteins like tau. In cancer abnormal UBE3C levels relate to changes in protein degradation that can lead to unchecked cell growth and survival affecting proteins like p53 an important tumor suppressor.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 ubiquitin-protein ligase that specifically catalyzes 'Lys-29'- and 'Lys-48'-linked polyubiquitin chains (PubMed : 11278995, PubMed : 12692129, PubMed : 16341092, PubMed : 16601690, PubMed : 24158444, PubMed : 24811749, PubMed : 25752573, PubMed : 25752577, PubMed : 32039437, PubMed : 33637724, PubMed : 34239127). Accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed : 32039437, PubMed : 9575161). Associates with the proteasome and promotes elongation of ubiquitin chains on substrates bound to the 26S proteasome (PubMed : 24158444, PubMed : 28396413, PubMed : 31375563). Also catalyzes 'Lys-29'- and 'Lys-48'-linked ubiquitination of 26S proteasome subunit ADRM1/RPN13 in response to proteotoxic stress, impairing the ability of the proteasome to bind and degrade ubiquitin-conjugated proteins (PubMed : 24811749, PubMed : 31375563). Acts as a negative regulator of autophagy by mediating 'Lys-29'- and 'Lys-48'-linked ubiquitination of PIK3C3/VPS34, promoting its degradation (PubMed : 33637724). Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains; with some preference for 'Lys-48' linkages (PubMed : 11278995, PubMed : 16601690, PubMed : 25752577). Acts as a negative regulator of type I interferon by mediating 'Lys-48'-linked ubiquitination of IRF3 and IRF7, leading to their degradation by the proteasome (PubMed : 21167755). Catalyzes ubiquitination and degradation of CAND2 (PubMed : 12692129).

See full target information UBE3C

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 41:261 PubMed36028854

2022

ALKBH5-mediated m6A modification of circCCDC134 facilitates cervical cancer metastasis by enhancing HIF1A transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Leilei Liang,Yunshu Zhu,Jian Li,Jia Zeng,Lingying Wu

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Product promise

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Anti-UBE3C antibody

Immunoprecipitation - Anti-UBE3C antibody (AB177511)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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