AB226173

Anti-UBE3C antibody

Name: Anti-UBE3C antibody (ab226173) | Abcam
Brand: Abcam Limited
SKU: AB226173
Price: 525 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal UBE3C antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human UBE3C aa 350-400.

View Alternative Names

KIAA0010, KIAA10, UBE3C, Ubiquitin-protein ligase E3C, HECT-type ubiquitin transferase E3C, Homologous to E6AP carboxyl terminus homologous protein 2, RTA-associated ubiquitin ligase, HectH2, RAUL

2 Images

Supplier Data

Immunoprecipitation - Anti-UBE3C antibody (AB226173)

UBE3C was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226173 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226173 at 0.4 μg/ml.

Lane 1 : ab226173 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-UBE3C antibody (ab226173)

Predicted band size: 124 kDa

false

Supplier Data

Western blot - Anti-UBE3C antibody (AB226173)

All lanes:

Western blot - Anti-UBE3C antibody (ab226173) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 124 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human UBE3C aa 350-400. The exact immunogen used to generate this antibody is proprietary information.

Q15386

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab226173 was affinity purified using an epitope specific to UBE3C immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBE3C also known as ubiquitin protein ligase E3C is an E3 ubiquitin-protein ligase that plays a mechanical role in tagging proteins for degradation. This tagging involves attaching ubiquitin molecules to substrate proteins which targets them for proteasomal degradation. UBE3C has a molecular mass of approximately 90 kDa. Expression of this protein occurs in various tissues with higher levels found in the brain and testis suggesting these organs may rely more on its function.

Biological function summary

UBE3C regulates protein homeostasis by working as part of the ubiquitin-proteasome system which maintains cellular function by removing damaged or unneeded proteins. It assists in controlling protein quality and stability. Although not typically part of larger multiprotein complexes UBE3C influences the turnover of several cellular proteins. Its action affects many processes including neuronal function and response to cellular stress.

Pathways

UBE3C activity intersects with the ubiquitin-proteasome pathway a fundamental mechanism for protein degradation and turnover in cells. This pathway ensures cellular protein quality and prevents toxic protein accumulation. It has associations with proteins like UCHL1 which also have roles in the ubiquitination process. Moreover UBE3C links to the Wnt signaling pathway by modulating the stability of pathway components such as β-catenin which influences cell proliferation and differentiation.

UBE3C involvement appears significant in neurodegenerative diseases and certain cancers. In neurodegenerative conditions like Alzheimer's disease altered UBE3C expression or function might impact protein aggregates formation similar to its interaction with proteins like tau. In cancer abnormal UBE3C levels relate to changes in protein degradation that can lead to unchecked cell growth and survival affecting proteins like p53 an important tumor suppressor.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 ubiquitin-protein ligase that specifically catalyzes 'Lys-29'- and 'Lys-48'-linked polyubiquitin chains (PubMed : 11278995, PubMed : 12692129, PubMed : 16341092, PubMed : 16601690, PubMed : 24158444, PubMed : 24811749, PubMed : 25752573, PubMed : 25752577, PubMed : 32039437, PubMed : 33637724, PubMed : 34239127). Accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed : 32039437, PubMed : 9575161). Associates with the proteasome and promotes elongation of ubiquitin chains on substrates bound to the 26S proteasome (PubMed : 24158444, PubMed : 28396413, PubMed : 31375563). Also catalyzes 'Lys-29'- and 'Lys-48'-linked ubiquitination of 26S proteasome subunit ADRM1/RPN13 in response to proteotoxic stress, impairing the ability of the proteasome to bind and degrade ubiquitin-conjugated proteins (PubMed : 24811749, PubMed : 31375563). Acts as a negative regulator of autophagy by mediating 'Lys-29'- and 'Lys-48'-linked ubiquitination of PIK3C3/VPS34, promoting its degradation (PubMed : 33637724). Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains; with some preference for 'Lys-48' linkages (PubMed : 11278995, PubMed : 16601690, PubMed : 25752577). Acts as a negative regulator of type I interferon by mediating 'Lys-48'-linked ubiquitination of IRF3 and IRF7, leading to their degradation by the proteasome (PubMed : 21167755). Catalyzes ubiquitination and degradation of CAND2 (PubMed : 12692129).

See full target information UBE3C

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

World journal of stem cells 17:100359 PubMed40308888

2025

Exosomal miR-137-3p targets UBE3C to activate STAT3, promoting migration and differentiation into endometrial epithelial cell of human umbilical cord mesenchymal stem cells under hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Wan-Yu Zhang,Si-Miao Liu,Han-Bi Wang,Cheng-Yan Deng

Autophagy 19:1512-1532 PubMed36343628

2022

Tegument protein UL21 of alpha-herpesvirus inhibits the innate immunity by triggering CGAS degradation through TOLLIP-mediated selective autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Zicheng Ma,Juan Bai,Chenlong Jiang,Huixin Zhu,Depeng Liu,Mengjiao Pan,Xianwei Wang,Jiang Pi,Ping Jiang,Xing Liu

Nature communications 11:6316 PubMed33298911

2020

TRIB3 promotes MYC-associated lymphoma development through suppression of UBE3B-mediated MYC degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Li,Feng Wang,Zhao-Na Yang,Ting-Ting Zhang,Yu-Fen Yuan,Chen-Xi Zhao,Zaiwuli Yeerjiang,Bing Cui,Fang Hua,Xiao-Xi Lv,Xiao-Wei Zhang,Jiao-Jiao Yu,Shan-Shan Liu,Jin-Mei Yu,Shuang Shang,Yang Xiao,Zhuo-Wei Hu

Cell death discovery 6:99 PubMed33083020

2020

LINC00355 induces gastric cancer proliferation and invasion through promoting ubiquitination of P53.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Zhao,Yan Jin,Peng Wu,Jian Yang,Yuanyuan Chen,Qianlu Yang,Xinying Huo,Juxue Li,Wei De,Jinfei Chen,Fen Yang

Molecular cell 79:768-781.e7 PubMed32738194

2020

Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Michael L van de Weijer,Logesvaran Krshnan,Sabrina Liberatori,Elena Navarro Guerrero,Jacob Robson-Tull,Lilli Hahn,Robert Jan Lebbink,Emmanuel J H J Wiertz,Roman Fischer,Daniel Ebner,Pedro Carvalho

International journal of oncology 53:266-274 PubMed29749468

2018

Inhibition of CD9 expression reduces the metastatic capacity of human hepatocellular carcinoma cell line MHCC97-H.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Lin,Shifang Peng,Yixuan Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UBE3C antibody

Immunoprecipitation - Anti-UBE3C antibody (AB226173)

Western blot - Anti-UBE3C antibody (AB226173)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise