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AB128861

Anti-Ube4a antibody [EPR7331]

2

(3 Reviews)

|

(3 Publications)

Rabbit Recombinant Monoclonal UBE4A antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

KIAA0126, UBE4A, Ubiquitin conjugation factor E4 A, RING-type E3 ubiquitin transferase E4 A

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Ube4a antibody [EPR7331] (AB128861)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Ube4a antibody [EPR7331] (AB128861)

ab128861, at 1/100 dilution, staining Ube4a in Caco 2 cells by Immunofluorescence.

Western blot - Anti-Ube4a antibody [EPR7331] (AB128861)
  • WB

Unknown

Western blot - Anti-Ube4a antibody [EPR7331] (AB128861)

All lanes:

Western blot - Anti-Ube4a antibody [EPR7331] (ab128861) at 1/1000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

Caco-2 cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 123 kDa

Observed band size: 125 kDa

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OI-RD Scanning - Anti-Ube4a antibody [EPR7331] (AB128861)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Ube4a antibody [EPR7331] (AB128861)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-Ube4a antibody [EPR7331] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7331

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ube4a also known as U-box containing E3 ubiquitin protein ligase 4A plays a role in the ubiquitination process. It catalyzes the transfer of ubiquitin molecules to specific substrates tagging them for degradation by the proteasome. Ube4a has an approximate molecular mass of 121 kDa. Researchers have observed its expression in various tissues indicating a potential role in widespread cellular processes.
Biological function summary

Ube4a functions in the regulation of protein quality and turnover. It forms part of a ubiquitin-proteasome system playing an important role in maintaining protein homeostasis within cells. The regulation of protein levels is important for various cellular processes including cell cycle control and signal transduction. Ube4a engages in multi-subunit complexes interacting with other E3 ligases to facilitate substrate ubiquitination.

Pathways

Ube4a integrates into the ubiquitin-proteasome pathway a central component in protein degradation. This pathway is essential for regulating various cellular processes including responses to stress. Ube4a works alongside E1 activating enzymes and E2 conjugating enzymes to deliver ubiquitin to substrates efficiently. Its role within this pathway emphasizes the modulation of protein function and turnover linking it with other proteins like USP5 and UCH37 which are involved in deubiquitination.

Ube4a associates with certain neurological conditions and cancer. Researchers have linked aberrant ubiquitination to the pathology of these diseases indicating the importance of Ube4a in maintaining cellular balance. In neurodegenerative disorders improper Ube4a activity may disrupt protein degradation implicating proteins like Parkin. In some cancer types altered Ube4a expression can affect cell cycle regulation with downstream impacts on other proteins such as p53 highlighting its potential involvement in tumorigenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. Mediates 'Lys-48'-linked polyubiquitination of substrates.
See full target information UBE4A
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 16:e1008773 PubMed32881988

2020

NS5-V372A and NS5-H386Y variations are responsible for differences in interferon α/β induction and co-contribute to the replication advantage of Japanese encephalitis virus genotype I over genotype III in ducklings.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxi Li,Di Di,Hui Huang,Xin Wang,Qiqi Xia,Xiaochun Ma,Ke Liu,Beibei Li,Donghua Shao,Yafeng Qiu,Zongjie Li,Jianchao Wei,Zhiyong Ma

Molecular cell 69:866-878.e7 PubMed29499138

2018

The Ubiquitin E3/E4 Ligase UBE4A Adjusts Protein Ubiquitylation and Accumulation at Sites of DNA Damage, Facilitating Double-Strand Break Repair.

Applications

Unspecified application

Species

Unspecified reactive species

Keren Baranes-Bachar,Adva Levy-Barda,Judith Oehler,Dylan A Reid,Isabel Soria-Bretones,Ty C Voss,Dudley Chung,Yoon Park,Chao Liu,Jong-Bok Yoon,Wei Li,Graham Dellaire,Tom Misteli,Pablo Huertas,Eli Rothenberg,Kristijan Ramadan,Yael Ziv,Yosef Shiloh

Journal of genetics and genomics = Yi chuan xue ba 45:111-120 PubMed29502958

2018

AQR is a novel type 2 diabetes-associated gene that regulates signaling pathways critical for glucose metabolism.

Applications

WB

Species

Human

Chun Song,Han Yan,Hanming Wang,Yan Zhang,Huiqing Cao,Yiqi Wan,Lingbao Kong,Shenghan Chen,Hong Xu,Bingxing Pan,Jin Zhang,Guohuang Fan,Hongbo Xin,Zicai Liang,Weiping Jia,Xiao-Li Tian
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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