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AB248800

Anti-Ube4a antibody [EPR7332] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal UBE4A antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA0126, UBE4A, Ubiquitin conjugation factor E4 A, RING-type E3 ubiquitin transferase E4 A

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Ube4a antibody [EPR7332] - BSA and Azide free (AB248800)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Ube4a antibody [EPR7332] - BSA and Azide free (AB248800)

This data was developed using ab137074, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells labelling Ube4a with ab137074 at 1/100 dilution.

Western blot - Anti-Ube4a antibody [EPR7332] - BSA and Azide free (AB248800)
  • WB

Unknown

Western blot - Anti-Ube4a antibody [EPR7332] - BSA and Azide free (AB248800)

This data was developed using ab137074, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Ube4a antibody [EPR7332] - BSA and Azide free (ab248800) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

Caco-2 cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 123 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7332

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab248800 is the carrier-free version of ab137074.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ube4a also known as U-box containing E3 ubiquitin protein ligase 4A plays a role in the ubiquitination process. It catalyzes the transfer of ubiquitin molecules to specific substrates tagging them for degradation by the proteasome. Ube4a has an approximate molecular mass of 121 kDa. Researchers have observed its expression in various tissues indicating a potential role in widespread cellular processes.
Biological function summary

Ube4a functions in the regulation of protein quality and turnover. It forms part of a ubiquitin-proteasome system playing an important role in maintaining protein homeostasis within cells. The regulation of protein levels is important for various cellular processes including cell cycle control and signal transduction. Ube4a engages in multi-subunit complexes interacting with other E3 ligases to facilitate substrate ubiquitination.

Pathways

Ube4a integrates into the ubiquitin-proteasome pathway a central component in protein degradation. This pathway is essential for regulating various cellular processes including responses to stress. Ube4a works alongside E1 activating enzymes and E2 conjugating enzymes to deliver ubiquitin to substrates efficiently. Its role within this pathway emphasizes the modulation of protein function and turnover linking it with other proteins like USP5 and UCH37 which are involved in deubiquitination.

Ube4a associates with certain neurological conditions and cancer. Researchers have linked aberrant ubiquitination to the pathology of these diseases indicating the importance of Ube4a in maintaining cellular balance. In neurodegenerative disorders improper Ube4a activity may disrupt protein degradation implicating proteins like Parkin. In some cancer types altered Ube4a expression can affect cell cycle regulation with downstream impacts on other proteins such as p53 highlighting its potential involvement in tumorigenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. Mediates 'Lys-48'-linked polyubiquitination of substrates.
See full target information UBE4A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

NPJ precision oncology 7:95 PubMed37723227

2023

Targeted single-cell proteomic analysis identifies new liquid biopsy biomarkers associated with multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia M Setayesh,Libere J Ndacayisaba,Kate E Rappard,Valerie Hennes,Luz Yurany Moreno Rueda,Guilin Tang,Pei Lin,Robert Z Orlowski,David E Symer,Elisabet E Manasanch,Stephanie N Shishido,Peter Kuhn
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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