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AB97697

Anti-UBE4B antibody

3

(1 Review)

|

(5 Publications)

Rabbit Polyclonal UBE4B antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human UBE4B aa 700-1050.

View Alternative Names

HDNB1, KIAA0684, UFD2, UBE4B, Ubiquitin conjugation factor E4 B, Homozygously deleted in neuroblastoma 1, RING-type E3 ubiquitin transferase E4 B, Ubiquitin fusion degradation protein 2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE4B antibody (AB97697)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE4B antibody (AB97697)

Immunohistochemical analysis of UBE4B in paraffin-embedded CA922 xenograft, using ab97697 at 1/500 dilution.

Western blot - Anti-UBE4B antibody (AB97697)
  • WB

Unknown

Western blot - Anti-UBE4B antibody (AB97697)

5% SDS PAGE

All lanes:

Western blot - Anti-UBE4B antibody (ab97697) at 1/10000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 133 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human UBE4B aa 700-1050. The exact immunogen used to generate this antibody is proprietary information.

O95155

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The UBE4B protein also known as U-box protein 2 (UFD2) is a human protein that plays an important role in the ubiquitination process. This protein with a molecular weight of approximately 139 kDa functions as an E4 ubiquitin ligase. UBE4B facilitates polyubiquitin chain assembly on substrates that have already been ubiquitinated by E3 ligases. It is expressed ubiquitously in human tissues though its expression levels can vary across different cell types.
Biological function summary

UBE4B is important in the regulation of protein homeostasis and cellular stress responses. It acts as a part of a complex with other ubiquitin-proteasome system components enhancing their capacity to mark proteins for degradation. This regulation is important for maintaining cellular health as it aids in disposing of misfolded or damaged proteins. UBE4B has also been implicated in DNA repair processes showcasing its diverse biological functions.

Pathways

UBE4B is involved in the ubiquitin-proteasome pathway an essential mechanism for protein turnover in the cell. This protein plays a role in the degradation of key regulatory proteins which influences cell cycle control and signal transduction pathways such as the p53 pathway. UBE4B interacts with proteins like MDM2 which is involved in the regulation of tumor suppressor p53 thereby connecting UBE4B's functionality to broader cellular processes.

UBE4B has associations with cancer and neurodegenerative diseases. Its role in the p53 pathway ties it closely to various forms of cancer as disruptions in p53 regulation can lead to tumor progression. In neurodegenerative diseases UBE4B's involvement in protein degradation implicates it in conditions like Parkinson's disease where protein misfolding and aggregation are pathological hallmarks. The protein's relationship with others like Parkin further emphasizes its relevance in the context of these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases (By similarity). May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase (By similarity). May regulate myosin assembly in striated muscles together with STUB1 and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation (PubMed : 17369820).
See full target information UBE4B
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Genes, chromosomes & cancer 62:313-331 PubMed36680522

2023

Identification of a novel gene signature for neuroblastoma differentiation using a Boolean implication network.

Applications

Unspecified application

Species

Unspecified reactive species

Peter E Zage,Yuchen Huo,Divya Subramonian,Christophe Le Clorennec,Pradipta Ghosh,Debashis Sahoo

Molecular therapy. Nucleic acids 31:466-481 PubMed36865087

2023

The SWIB/MDM2 motif of UBE4B activates the p53 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

H Helena Wu,Sarah Leng,Yasser Abuetabh,Consolato Sergi,David D Eisenstat,Roger Leng

EMBO molecular medicine 14:e14552 PubMed35174975

2022

A clinically compatible drug-screening platform based on organotypic cultures identifies vulnerabilities to prevent and treat brain metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Lucía Zhu,Diana Retana,Pedro García-Gómez,Laura Álvaro-Espinosa,Neibla Priego,Mariam Masmudi-Martín,Natalia Yebra,Lauritz Miarka,Elena Hernández-Encinas,Carmen Blanco-Aparicio,Sonia Martínez,Cecilia Sobrino,Nuria Ajenjo,Maria-Jesus Artiga,Eva Ortega-Paino,Raúl Torres-Ruiz,Sandra Rodríguez-Perales,Riccardo Soffietti,Luca Bertero,Paola Cassoni,Tobias Weiss,Javier Muñoz,Juan Manuel Sepúlveda,Pedro González-León,Luis Jiménez-Roldán,Luis Miguel Moreno,Olga Esteban,Ángel Pérez-Núñez,Aurelio Hernández-Laín,Oscar Toldos,Yolanda Ruano,Lucía Alcázar,Guillermo Blasco,José Fernández-Alén,Eduardo Caleiras,Miguel Lafarga,Diego Megías,Osvaldo Graña-Castro,Carolina Nör,Michael D Taylor,Leonie S Young,Damir Varešlija,Nicola Cosgrove,Fergus J Couch,Lorena Cussó,Manuel Desco,Silvana Mouron,Miguel Quintela-Fandino,Michael Weller,Joaquín Pastor,Manuel Valiente

Molecular and cellular neurosciences 108:103542 PubMed32841720

2020

The ubiquitin ligase UBE4B regulates amyloid precursor protein ubiquitination, endosomal trafficking, and amyloid β42 generation and secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Monica Gireud-Goss,Sahily Reyes,Ritika Tewari,Anthony Patrizz,Matthew D Howe,Julia Kofler,M Neal Waxham,Louise D McCullough,Andrew J Bean

Genes & cancer 7:13-26 PubMed27014418

2016

Neuroblastoma patient outcomes, tumor differentiation, and ERK activation are correlated with expression levels of the ubiquitin ligase UBE4B.

Applications

IHC, WB

Species

Unspecified reactive species, Unspecified reactive species

Sarah E Woodfield,Rong Jun Guo,Yin Liu,Angela M Major,Emporia Faith Hollingsworth,Sandra Indiviglio,Sarah B Whittle,Qianxing Mo,Andrew J Bean,Michael Ittmann,Dolores Lopez-Terrada,Peter E Zage
View all publications
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