JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB126759

Anti-UBE4B antibody [EPR7471]

4

(2 Reviews)

|

(8 Publications)

Rabbit Recombinant Monoclonal UBE4B antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 8 publications.

View Alternative Names

HDNB1, KIAA0684, UFD2, UBE4B, Ubiquitin conjugation factor E4 B, Homozygously deleted in neuroblastoma 1, RING-type E3 ubiquitin transferase E4 B, Ubiquitin fusion degradation protein 2

2 Images
Western blot - Anti-UBE4B antibody [EPR7471] (AB126759)
  • WB

Unknown

Western blot - Anti-UBE4B antibody [EPR7471] (AB126759)

All lanes:

Western blot - Anti-UBE4B antibody [EPR7471] (ab126759) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

Raji cell lysate at 10 µg

Lane 3:

SK OV 3 cell lysate at 10 µg

Lane 4:

L929 cell lysate at 10 µg

Lane 5:

L6 cell lysate at 10 µg

Lane 6:

RAW264.7 cell lysate at 10 µg

Lane 7:

PC12 cell lysate at 10 µg

Lane 8:

NIH 3T3 cell lysate at 10 µg

Lane 9:

HeLa cell lysate at 10 µg

Lane 10:

K562 cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit at 1/2000 dilution

Predicted band size: 146 kDa

false

Western blot - Anti-UBE4B antibody [EPR7471] (AB126759)
  • WB

AbReview40444****

Western blot - Anti-UBE4B antibody [EPR7471] (AB126759)

Western blot analysis of Human 293NT whole cell lysate labeling UBE4B with ab126759 at 1/2000. A HRP-conjugated goat anti-rabbit IgG polyclonal (1/20000) was used as the secondary antibody.

siRNA agains UBE4B was used to show for antibody specificity.

Unspecific siRNA (siLUC) was used as a negative control.

All lanes:

Western blot - Anti-UBE4B antibody [EPR7471] (ab126759)

Predicted band size: 146 kDa

true

Exposure time: 1min

This image is courtesy of an anonymous Abreview

  • Carrier free

    Anti-UBE4B antibody [EPR7471] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7471

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The UBE4B protein also known as U-box protein 2 (UFD2) is a human protein that plays an important role in the ubiquitination process. This protein with a molecular weight of approximately 139 kDa functions as an E4 ubiquitin ligase. UBE4B facilitates polyubiquitin chain assembly on substrates that have already been ubiquitinated by E3 ligases. It is expressed ubiquitously in human tissues though its expression levels can vary across different cell types.
Biological function summary

UBE4B is important in the regulation of protein homeostasis and cellular stress responses. It acts as a part of a complex with other ubiquitin-proteasome system components enhancing their capacity to mark proteins for degradation. This regulation is important for maintaining cellular health as it aids in disposing of misfolded or damaged proteins. UBE4B has also been implicated in DNA repair processes showcasing its diverse biological functions.

Pathways

UBE4B is involved in the ubiquitin-proteasome pathway an essential mechanism for protein turnover in the cell. This protein plays a role in the degradation of key regulatory proteins which influences cell cycle control and signal transduction pathways such as the p53 pathway. UBE4B interacts with proteins like MDM2 which is involved in the regulation of tumor suppressor p53 thereby connecting UBE4B's functionality to broader cellular processes.

UBE4B has associations with cancer and neurodegenerative diseases. Its role in the p53 pathway ties it closely to various forms of cancer as disruptions in p53 regulation can lead to tumor progression. In neurodegenerative diseases UBE4B's involvement in protein degradation implicates it in conditions like Parkinson's disease where protein misfolding and aggregation are pathological hallmarks. The protein's relationship with others like Parkin further emphasizes its relevance in the context of these disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases (By similarity). May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase (By similarity). May regulate myosin assembly in striated muscles together with STUB1 and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation (PubMed : 17369820).
See full target information UBE4B
style
left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurodegeneration 20:2 PubMed39773263

2025

VCP regulates early tau seed amplification via specific cofactors.

Applications

Unspecified application

Species

Unspecified reactive species

Sushobhna Batra,Jaime Vaquer-Alicea,Clarissa Valdez,Skyler P Taylor,Victor A Manon,Anthony R Vega,Omar M Kashmer,Sourav Kolay,Andrew Lemoff,Nigel J Cairns,Charles L White,Marc I Diamond

iScience 27:109870 PubMed38799573

2024

IGF2BP3 modified GLI2 transcriptionally regulates SYVN1 and facilitates sepsis liver injury through autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanzheng Sun,Min Gao,Haotian Hu,Jing Qi,Yishu Tang,Xiaoxue Cao,Runbang Zhang,Huaizheng Liu

FEBS letters 598:702-715 PubMed38439679

2024

Recruitment of ubiquitin E2 enzymes is determined jointly by the U-box domains and substrates of E3 ligases.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Jin,Bei Li,Junyao Qu,Yiheng Sun,Mengran Wang,Changjiang Yang,Yuchen Fan,Yanan Wang,Peng Xu,Haiying Sun,Bo Jiang,Bo Zhao

Development (Cambridge, England) 149: PubMed36440598

2022

Fine-tuning of mTOR signaling by the UBE4B-KLHL22 E3 ubiquitin ligase cascade in brain development.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangxing Kong,Xin Shu,Jiachuan Wang,Dandan Liu,Yingchun Ni,Weiqi Zhao,Lebo Wang,Zhihua Gao,Jiadong Chen,Bing Yang,Xing Guo,Zhiping Wang

Frontiers in cell and developmental biology 10:833396 PubMed35669517

2022

Differential Degradation of TRA2A and PYCR2 Mediated by Ubiquitin E3 Ligase E4B.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Lu,Bo Jiang,Kangli Peng,Shasha Li,Xiangnan Liu,Bufan Wang,Yuntian Chen,Tiepeng Wang,Bo Zhao

Molecular and cellular neurosciences 108:103542 PubMed32841720

2020

The ubiquitin ligase UBE4B regulates amyloid precursor protein ubiquitination, endosomal trafficking, and amyloid β42 generation and secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Monica Gireud-Goss,Sahily Reyes,Ritika Tewari,Anthony Patrizz,Matthew D Howe,Julia Kofler,M Neal Waxham,Louise D McCullough,Andrew J Bean

Cancer biology & therapy 20:1416-1429 PubMed31475882

2019

Low UBE4B expression increases sensitivity of chemoresistant neuroblastoma cells to EGFR and STAT5 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Kimiya Memarzadeh,David J Savage,Andrew J Bean

Molecular cell 69:866-878.e7 PubMed29499138

2018

The Ubiquitin E3/E4 Ligase UBE4A Adjusts Protein Ubiquitylation and Accumulation at Sites of DNA Damage, Facilitating Double-Strand Break Repair.

Applications

Unspecified application

Species

Unspecified reactive species

Keren Baranes-Bachar,Adva Levy-Barda,Judith Oehler,Dylan A Reid,Isabel Soria-Bretones,Ty C Voss,Dudley Chung,Yoon Park,Chao Liu,Jong-Bok Yoon,Wei Li,Graham Dellaire,Tom Misteli,Pablo Huertas,Eli Rothenberg,Kristijan Ramadan,Yael Ziv,Yosef Shiloh
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB97697

Anti-UBE4B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE4B antibody (AB97697)

  • 3
  • 1

Complementary Products

Primary Antibodies

AB134064

Anti-STUB1/CHIP antibody [EPR4447]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-STUB1/CHIP antibody [EPR4447] (AB134064)

  • 4
  • 9
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB179503

Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (AB179503)

  • 4
  • 3
Primary Antibodies

AB109012

Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

KO Validated|RabMAb|Recombinant|Lab Essentials

Western blot - Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB109012)

  • 5
  • 15

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com