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AB248171

Anti-UBE4B antibody [EPR7471] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal UBE4B antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

HDNB1, KIAA0684, UFD2, UBE4B, Ubiquitin conjugation factor E4 B, Homozygously deleted in neuroblastoma 1, RING-type E3 ubiquitin transferase E4 B, Ubiquitin fusion degradation protein 2

1 Images
Western blot - Anti-UBE4B antibody [EPR7471] - BSA and Azide free (AB248171)
  • WB

Unknown

Western blot - Anti-UBE4B antibody [EPR7471] - BSA and Azide free (AB248171)

This data was developed using ab126759, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-UBE4B antibody [EPR7471] (<a href='/en-us/products/primary-antibodies/ube4b-antibody-epr7471-ab126759'>ab126759</a>) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

Raji cell lysate at 10 µg

Lane 3:

SK OV 3 cell lysate at 10 µg

Lane 4:

L929 cell lysate at 10 µg

Lane 5:

L6 cell lysate at 10 µg

Lane 6:

RAW264.7 cell lysate at 10 µg

Lane 7:

PC12 cell lysate at 10 µg

Lane 8:

NIH 3T3 cell lysate at 10 µg

Lane 9:

HeLa cell lysate at 10 µg

Lane 10:

K562 cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit at 1/2000 dilution

Predicted band size: 146 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7471

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab248171 is the carrier-free version of ab126759.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The UBE4B protein also known as U-box protein 2 (UFD2) is a human protein that plays an important role in the ubiquitination process. This protein with a molecular weight of approximately 139 kDa functions as an E4 ubiquitin ligase. UBE4B facilitates polyubiquitin chain assembly on substrates that have already been ubiquitinated by E3 ligases. It is expressed ubiquitously in human tissues though its expression levels can vary across different cell types.
Biological function summary

UBE4B is important in the regulation of protein homeostasis and cellular stress responses. It acts as a part of a complex with other ubiquitin-proteasome system components enhancing their capacity to mark proteins for degradation. This regulation is important for maintaining cellular health as it aids in disposing of misfolded or damaged proteins. UBE4B has also been implicated in DNA repair processes showcasing its diverse biological functions.

Pathways

UBE4B is involved in the ubiquitin-proteasome pathway an essential mechanism for protein turnover in the cell. This protein plays a role in the degradation of key regulatory proteins which influences cell cycle control and signal transduction pathways such as the p53 pathway. UBE4B interacts with proteins like MDM2 which is involved in the regulation of tumor suppressor p53 thereby connecting UBE4B's functionality to broader cellular processes.

UBE4B has associations with cancer and neurodegenerative diseases. Its role in the p53 pathway ties it closely to various forms of cancer as disruptions in p53 regulation can lead to tumor progression. In neurodegenerative diseases UBE4B's involvement in protein degradation implicates it in conditions like Parkinson's disease where protein misfolding and aggregation are pathological hallmarks. The protein's relationship with others like Parkin further emphasizes its relevance in the context of these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases (By similarity). May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase (By similarity). May regulate myosin assembly in striated muscles together with STUB1 and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation (PubMed : 17369820).
See full target information UBE4B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:5945 PubMed36209205

2022

Hepatocyte phosphatase DUSP22 mitigates NASH-HCC progression by targeting FAK.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxu Ge,Jun Tan,Xianling Dai,Qin Kuang,Shaoyu Zhong,Lili Lai,Chao Yi,Yan Sun,Jing Luo,Chufeng Zhang,Liancai Zhu,Bochu Wang,Minxuan Xu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com