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AB317434

Anti-UBE4B Antibody [S04-6C6]

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Rabbit Monoclonal UBE4B antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human UBE4B.

View Alternative Names

HDNB1, KIAA0684, UFD2, UBE4B, Ubiquitin conjugation factor E4 B, Homozygously deleted in neuroblastoma 1, RING-type E3 ubiquitin transferase E4 B, Ubiquitin fusion degradation protein 2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-UBE4B Antibody [S04-6C6] (AB317434)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-UBE4B Antibody [S04-6C6] (AB317434)

Immunocytochemistry of UBE4B (green) in MCF-7 using ab317434 at dilution 1/20, and DAPI (blue).

Western blot - Anti-UBE4B Antibody [S04-6C6] (AB317434)
  • WB

Supplier Data

Western blot - Anti-UBE4B Antibody [S04-6C6] (AB317434)

All lanes:

Western blot - Anti-UBE4B Antibody [S04-6C6] (ab317434) at 1/1000 dilution

Lane 1:

HeLa cell lysate

Lane 2:

A549 cell lysate

Lane 3:

HL-60 cell lysate

Lane 4:

U2OS cell lysate

Lane 5:

U897-MG cell lysate

Predicted band size: 146 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

S04-6C6

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human UBE4B.

O95155

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/20", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.01% Sodium azide Constituents: 55.08% Water, 40% Glycerol (glycerin, glycerine), 0.98% Tris glycine, 0.88% Sodium chloride, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The UBE4B protein also known as U-box protein 2 (UFD2) is a human protein that plays an important role in the ubiquitination process. This protein with a molecular weight of approximately 139 kDa functions as an E4 ubiquitin ligase. UBE4B facilitates polyubiquitin chain assembly on substrates that have already been ubiquitinated by E3 ligases. It is expressed ubiquitously in human tissues though its expression levels can vary across different cell types.
Biological function summary

UBE4B is important in the regulation of protein homeostasis and cellular stress responses. It acts as a part of a complex with other ubiquitin-proteasome system components enhancing their capacity to mark proteins for degradation. This regulation is important for maintaining cellular health as it aids in disposing of misfolded or damaged proteins. UBE4B has also been implicated in DNA repair processes showcasing its diverse biological functions.

Pathways

UBE4B is involved in the ubiquitin-proteasome pathway an essential mechanism for protein turnover in the cell. This protein plays a role in the degradation of key regulatory proteins which influences cell cycle control and signal transduction pathways such as the p53 pathway. UBE4B interacts with proteins like MDM2 which is involved in the regulation of tumor suppressor p53 thereby connecting UBE4B's functionality to broader cellular processes.

UBE4B has associations with cancer and neurodegenerative diseases. Its role in the p53 pathway ties it closely to various forms of cancer as disruptions in p53 regulation can lead to tumor progression. In neurodegenerative diseases UBE4B's involvement in protein degradation implicates it in conditions like Parkinson's disease where protein misfolding and aggregation are pathological hallmarks. The protein's relationship with others like Parkin further emphasizes its relevance in the context of these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases (By similarity). May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase (By similarity). May regulate myosin assembly in striated muscles together with STUB1 and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation (PubMed : 17369820).
See full target information UBE4B
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