AB240696

Anti-UBFD1 antibody

Name: Anti-UBFD1 antibody (ab240696) | Abcam
Brand: Abcam Limited
SKU: AB240696

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(2 Publications)

Rabbit Polyclonal UBFD1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human UBFD1 aa 150-200.

View Alternative Names

UBPH, UBFD1, Ubiquitin domain-containing protein UBFD1, Ubiquitin-binding protein homolog

2 Images

Supplier Data

Immunoprecipitation - Anti-UBFD1 antibody (AB240696)

UBFD1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab240696 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab240696 at 1 μg/ml.

Lane 1 : ab240696 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HeK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-UBFD1 antibody (ab240696)

Predicted band size: 33 kDa

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Supplier Data

Western blot - Anti-UBFD1 antibody (AB240696)

All lanes:

Western blot - Anti-UBFD1 antibody (ab240696) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 33 kDa

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Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human UBFD1 aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

O14562

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab240696 was affinity purified using an epitope specific to UBFD1 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBFD1 also known as Ubiquitin Family Domain-Containing Protein 1 is a protein with a molecular mass of approximately 35 kDa. It functions mechanically by interacting with ubiquitin tags playing a role in protein degradation and localization through the ubiquitin-proteasome system. UBFD1 is expressed in various tissues with notable expression in the liver brain and immune cells. This widespread expression suggests its involvement in multiple cellular functions.

Biological function summary

UBFD1 engages in cellular homeostasis by influencing protein turnover and signaling pathways. It is not known as part of a traditional protein complex but it interacts with several ubiquitin-associated proteins. UBFD1 regulates the stability of proteins by mediating their ubiquitination status which affects the cell cycle DNA repair and response to stress conditions. Its role is important for maintaining normal cellular function and preventing the accumulation of damaged proteins.

Pathways

UBFD1 influences many cellular processes notably the ubiquitin-proteasome pathway and the DNA damage response pathway. It works closely with proteins such as ubiquitin-conjugating enzymes and substrate-specific ubiquitin ligases. These pathways are integral to maintaining protein homeostasis and ensuring proper cellular responses to damage and stress. By modulating these pathways UBFD1 ensures effective protein management and repair mechanisms within the cell.

UBFD1 is linked to neurodegenerative diseases and certain types of cancer. Abnormal regulation or mutations in UBFD1 may contribute to protein misfolding and aggregation seen in diseases like Alzheimer's. In cancer UBFD1 dysfunction may lead to unregulated cell division due to impaired protein degradation. Studies have shown interactions between UBFD1 and proteins such as p53 highlighting its relevance in cancer pathogenesis through disruption of normal cell cycle control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play a role as NF-kappa-B regulator.

See full target information UBFD1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 16:324 PubMed40597299

2025

Mesenchymal stromal cell secretome reduces lung injury and thrombo-inflammation induced by SARS-CoV-2 spike protein.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Pezzotta,Alessandra Bovio,Barbara Imberti,Monica Locatelli,Daniela Corna,Domenico Cerullo,Sara Gastoldi,Ariela Benigni,Giuseppe Remuzzi,Marina Morigi,Luca Perico

HGG advances 6:100391 PubMed39663698

2024

Whole-exome sequencing reveals known and candidate genes for hearing impairment in Mali.

Applications

Unspecified application

Species

Unspecified reactive species

Abdoulaye Yalcouyé,Isabelle Schrauwen,Oumou Traoré,Salia Bamba,Elvis Twumasi Aboagye,Anushree Acharya,Thashi Bharadwaj,Rachel Latanich,Kevin Esoh,Cesar A Fortes-Lima,Carmen de Kock,Mario Jonas,Alassane Dit Baneye Maiga,Cheick A K Cissé,Moussa A Sangaré,Cheick O Guinto,Guida Landouré,Suzanne M Leal,Ambroise Wonkam

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UBFD1 antibody

Immunoprecipitation - Anti-UBFD1 antibody (AB240696)

Western blot - Anti-UBFD1 antibody (AB240696)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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Product promise