Skip to main content

Mouse Monoclonal Ubiquinol-Cytochrome C Reductase Core Protein I antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Cow, Rat, Mouse samples. Cited in 105 publications.


Images

Western blot - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (AB110252), expandable thumbnail
  • Flow Cytometry - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (AB110252), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (AB110252), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBICC/IF
Human
Tested
Expected
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected
Cow
Expected
Tested
Expected

Tested
Tested

Species

Human

Dilution info

1 µg for 106 Cells

Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Cow, Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Cow

Dilution info

0.5 µg/mL

Notes

-

Species

Rat

Dilution info

0.5 µg/mL

Notes

-

Species

Mouse

Dilution info

0.5 µg/mL

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Expected
Expected

Species

Cow, Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c (By similarity). The 2 core subunits UQCRC1/QCR1 and UQCRC2/QCR2 are homologous to the 2 mitochondrial-processing peptidase (MPP) subunits beta-MPP and alpha-MPP respectively, and they seem to have preserved their MPP processing properties (By similarity). May be involved in the in situ processing of UQCRFS1 into the mature Rieske protein and its mitochondrial targeting sequence (MTS)/subunit 9 when incorporated into complex III (Probable). Seems to play an important role in the maintenance of proper mitochondrial function in nigral dopaminergic neurons (PubMed:33141179).

Alternative names

Recommended products

Mouse Monoclonal Ubiquinol-Cytochrome C Reductase Core Protein I antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Cow, Rat, Mouse samples. Cited in 105 publications.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

16D10AD9AH5

Purity

IgG fraction

Light chain type

kappa

Concentration
Loading...
Purification notes

ab110252 is produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product was previously marketed under the MitoSciences sub-brand.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Ubiquinol-Cytochrome C Reductase Core Protein I also known as Core Protein I of the cytochrome c reductase complex functions as part of the mitochondrial electron transport chain. It has a molecular weight of around 52 kDa. The protein plays an integral role within the inner mitochondrial membrane primarily performing mechanical roles in electron transfer. Ubiquinol-Cytochrome C Reductase Core Protein I also goes by its abbreviated symbol UQCRC1 and is expressed broadly across tissues with high energy demands.

Biological function summary

UQCRC1 acts as a core component of Complex III also referred to as Cytochrome bc1 complex. This complex sits centrally within the mitochondrial respiratory chain catalyzing the transfer of electrons from ubiquinol to cytochrome c. By facilitating this electron transfer UQCRC1 aids in generating a proton gradient across the inner mitochondrial membrane essential for ATP synthesis during oxidative phosphorylation.

Pathways

UQCRC1 finds itself prominently embedded in the oxidative phosphorylation and electron transport chain pathways. It directly interacts with other proteins such as cytochrome c and cytochrome reductase emphasizing its cooperative function in maintaining cellular energy balance. The seamless integration of UQCRC1 into these pathways highlights its importance in sustaining efficient mitochondrial operation a critical element for cellular respiration.

Associated diseases and disorders

UQCRC1 shows connections to conditions involving mitochondrial dysfunction such as Leigh syndrome and mitochondrial myopathy. These disorders sometimes exhibit impaired electron transport impacting ATP production. Proteins like cytochrome c and other cytochrome reductase components often participate in these disorder pathways indicating potential targets for therapeutic interventions that aim to restore or augment mitochondrial function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252), expandable thumbnail

    Western blot - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252)

    Extra bands in the Mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.

    All lanes: Western blot - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252) at 0.5 µg/mL

    Lane 1: Human heart mitochondrial lysate

    Lane 2: Cow heart mitochondrial lysate

    Lane 3: Rat heart mitochondrial lysate

    Lane 4: Mouse heart mitochondrial lysate

    Lane 5: HepG2 mitochondrial lysate

    Predicted band size: 53 kDa

  • Flow Cytometry - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252), expandable thumbnail

    Flow Cytometry - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252)

    Overlay histogram showing HepG2 cells stained with ab110252 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110252, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunocytochemistry/ Immunofluorescence - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252)

    ab110252 stained HCT116 cells. The cells were 100% methanol fixed for 5 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110252 at 1/50 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43μM for 1hour at room temperature.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com