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AB223746

Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody

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(1 Publication)

Rabbit Polyclonal Ubiquinol-Cytochrome C Reductase Core Protein I antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human UQCRC1 aa 100-300.

View Alternative Names

Complex III subunit 1, Core protein I, Ubiquinol-cytochrome-c reductase complex core protein 1, UQCRC1

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (AB223746)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (AB223746)

Paraffin-embedded human lymph node tissue stained for UQCRC1 using ab223746 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (AB223746)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (AB223746)

Paraffin-embedded human small intestine tissue stained for UQCRC1 using ab223746 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (AB223746)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (AB223746)

Paraffin-embedded human heart muscle tissue stained for UQCRC1 using ab223746 at 1/200 dilution in immunohistochemical analysis.

Western blot - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (AB223746)
  • WB

Supplier Data

Western blot - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (AB223746)

All lanes:

Western blot - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (ab223746) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate

Predicted band size: 53 kDa

false

Western blot - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (AB223746)
  • WB

Supplier Data

Western blot - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (AB223746)

All lanes:

Western blot - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (ab223746) at 1/100 dilution

All lanes:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human UQCRC1 aa 100-300. The exact immunogen used to generate this antibody is proprietary information.

P31930

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ubiquinol-Cytochrome C Reductase Core Protein I also known as Core Protein I of the cytochrome c reductase complex functions as part of the mitochondrial electron transport chain. It has a molecular weight of around 52 kDa. The protein plays an integral role within the inner mitochondrial membrane primarily performing mechanical roles in electron transfer. Ubiquinol-Cytochrome C Reductase Core Protein I also goes by its abbreviated symbol UQCRC1 and is expressed broadly across tissues with high energy demands.
Biological function summary

UQCRC1 acts as a core component of Complex III also referred to as Cytochrome bc1 complex. This complex sits centrally within the mitochondrial respiratory chain catalyzing the transfer of electrons from ubiquinol to cytochrome c. By facilitating this electron transfer UQCRC1 aids in generating a proton gradient across the inner mitochondrial membrane essential for ATP synthesis during oxidative phosphorylation.

Pathways

UQCRC1 finds itself prominently embedded in the oxidative phosphorylation and electron transport chain pathways. It directly interacts with other proteins such as cytochrome c and cytochrome reductase emphasizing its cooperative function in maintaining cellular energy balance. The seamless integration of UQCRC1 into these pathways highlights its importance in sustaining efficient mitochondrial operation a critical element for cellular respiration.

UQCRC1 shows connections to conditions involving mitochondrial dysfunction such as Leigh syndrome and mitochondrial myopathy. These disorders sometimes exhibit impaired electron transport impacting ATP production. Proteins like cytochrome c and other cytochrome reductase components often participate in these disorder pathways indicating potential targets for therapeutic interventions that aim to restore or augment mitochondrial function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c (By similarity). The 2 core subunits UQCRC1/QCR1 and UQCRC2/QCR2 are homologous to the 2 mitochondrial-processing peptidase (MPP) subunits beta-MPP and alpha-MPP respectively, and they seem to have preserved their MPP processing properties (By similarity). May be involved in the in situ processing of UQCRFS1 into the mature Rieske protein and its mitochondrial targeting sequence (MTS)/subunit 9 when incorporated into complex III (Probable). Seems to play an important role in the maintenance of proper mitochondrial function in nigral dopaminergic neurons (PubMed : 33141179).
See full target information UQCRC1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature : PubMed38057666

2023

A human embryonic limb cell atlas resolved in space and time.

Applications

Unspecified application

Species

Unspecified reactive species

Bao Zhang,Peng He,John E G Lawrence,Shuaiyu Wang,Elizabeth Tuck,Brian A Williams,Kenny Roberts,Vitalii Kleshchevnikov,Lira Mamanova,Liam Bolt,Krzysztof Polanski,Tong Li,Rasa Elmentaite,Eirini S Fasouli,Martin Prete,Xiaoling He,Nadav Yayon,Yixi Fu,Hao Yang,Chen Liang,Hui Zhang,Raphael Blain,Alain Chedotal,David R FitzPatrick,Helen Firth,Andrew Dean,Omer Ali Bayraktar,John C Marioni,Roger A Barker,Mekayla A Storer,Barbara J Wold,Hongbo Zhang,Sarah A Teichmann
View all publications
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