AB223378

Anti-Ubiquitin antibody [5B9-B3]

Name: Anti-Ubiquitin antibody [5B9-B3] (ab223378) | Abcam
Brand: Abcam Limited
SKU: AB223378

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(3 Publications)

Mouse Monoclonal UBB antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Native Full Length Protein corresponding to Cow UBB conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Polyubiquitin-B, UBB

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin antibody [5B9-B3] (AB223378)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin antibody [5B9-B3] (AB223378)

2% formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Ubiquitin (green) using ab223378 at 1/100 dilution in ICC/IF. Secondary : Goat Anti-Mouse FITC at 1/200 dilution for 2 hours at RT. Counterstain : DAPI (blue) nuclear stain at 1/40000 for 2 hours RT.

(A) DAPI nuclear stain (B) ab223378 (C) Merge.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin antibody [5B9-B3] (AB223378)

(A) DAPI nuclear stain (B) ab223378 (C) Merge.

Supplier Data

Western blot - Anti-Ubiquitin antibody [5B9-B3] (AB223378)

Blocking buffer : 1.5% BSA.

Primary antibody was incubated for 2 hours, the secondary for 1 hour at RT.

Mix of human cell line lysates : epidermoid carcinoma (A431), adenocarcinomic alveolar basal epithelial cells (A549), colon cancer (HCT116), cervical cancer (HeLa), embryonic kidney (HEK293), liver cancer (HepG2), promyelocytic leukemia (HL-60), umbilical vein endothelial cells (HUVEC), T lymphocytes (Jurkat), breast cancer (MCF7), prostate cancer (PC3), brain glioblastom (T98G).

All lanes:

Western blot - Anti-Ubiquitin antibody [5B9-B3] (ab223378) at 1/1000 dilution

All lanes:

Mix of human cell lysates at 15 µg

Secondary

All lanes:

Sheep Anti-Mouse IgG HRP

Predicted band size: 26 kDa

Observed band size: 10 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5B9-B3

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Native Full Length Protein corresponding to Cow UBB conjugated to Keyhole Limpet Haemocyanin.

P0CG53

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ubiquitin is a small regulatory protein found in almost all tissues of eukaryotic organisms. It has a molecular weight of approximately 8.6 kilodaltons. It functions mechanically by attaching to proteins through a process called ubiquitination which involves the formation of an isopeptide bond. Ubiquitin molecules can form polyubiquitin chains through different lysine residues such as K48 and K63 that determine their function. These chains label substrate proteins for various fates including degradation. Ubiquitin is expressed ubiquitously in cells reflecting its essential role in maintaining protein homeostasis.

Biological function summary

The ubiquitin system plays a critical role in regulating protein turnover and quality control within cells. It is part of a larger complex known as the ubiquitin-proteasome system (UPS) which is responsible for degrading proteins that need to be turned over. This process is essential for cell cycle control response to oxidative stress and DNA repair. Ubiquitin's role in tagging proteins for degradation or signaling allows cells to respond quickly to changes in their environment and maintain balance.

Pathways

Ubiquitin functions in several important biological pathways including the Wnt and NF-kB pathways. In the Wnt signaling pathway ubiquitination modulates the stability of key components thereby affecting the pathway's overall activity. In the NF-kB signaling pathway ubiquitin labels inhibitor proteins for degradation which releases and activates NF-kB. These pathways highlight ubiquitin's interaction with proteins such as beta-catenin in Wnt and IkB in NF-kB illustrating how it regulates diverse cellular processes.

The dysfunction of the ubiquitin system is linked to neurodegenerative diseases and cancers. Ubiquitin-related defects in protein degradation can lead to the buildup of unwanted proteins contributing to conditions like Parkinson's disease. Connections with cancer are evident as ubiquitin controls cell cycle proteins and aberrant ubiquitination may drive tumor growth and progression. The protein p53 known to be controlled by ubiquitination plays a significant role in cancer related mechanisms when dysregulated. Understanding and targeting ubiquitin-related pathways may provide new therapeutic opportunities for treating these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ubiquitin. Exists either covalently attached to another protein, or free (unanchored) (By similarity). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains) (PubMed : 26116755). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked : Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in proteotoxic stress response and cell cycle; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B (By similarity). Linear polymer chains formed via attachment by the initiator Met lead to cell signaling (By similarity). Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed (By similarity). When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling (By similarity).

See full target information UBB

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 82:2858-2870.e8 PubMed35732190

2022

Supramolecular assembly of GSK3α as a cellular response to amino acid starvation.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Hinze,Sabine Schreek,Andre Zeug,Nurul Khalida Ibrahim,Beate Fehlhaber,Lorent Loxha,Buesra Cinar,Evgeni Ponimaskin,James Degar,Connor McGuckin,Gabriela Chiosis,Cornelia Eckert,Gunnar Cario,Beat Bornhauser,Jean-Pierre Bourquin,Martin Stanulla,Alejandro Gutierrez

Frontiers in neuroscience 16:907155 PubMed35769705

2022

Adenosine Downregulates the Activities of Glutamatergic Neurons in the Paraventricular Hypothalamic Nucleus Required for Sleep.

Applications

Unspecified application

Species

Unspecified reactive species

Changlin Chen,Yichen Lin,Feng Cai,Jinsui Li,Haixun Li,Xiantao Li

Microbiology spectrum 10:e0071822 PubMed35768946

2022

-Induced Mitophagy That Balances Mitochondrial Homeostasis and mROS-Mediated Bactericidal Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Jiao,Shiyang Cao,Yuan Zhang,Yafang Tan,Yazhou Zhou,Tong Wang,Yang You,Hongyan Chen,Yifan Ren,Ruifu Yang,Zongmin Du

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ubiquitin antibody [5B9-B3]

Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin antibody [5B9-B3] (AB223378)

Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin antibody [5B9-B3] (AB223378)

Western blot - Anti-Ubiquitin antibody [5B9-B3] (AB223378)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise