Rabbit Polyclonal UBB antibody. Suitable for WB and reacts with Human, Mouse, Saccharomyces cerevisiae samples. Cited in 60 publications. Immunogen corresponding to Native Full Length Protein corresponding to Cow UBB conjugated to Keyhole Limpet Haemocyanin.
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- Cells 12:2023Dysregulation of Metabolism and Proteostasis in Skeletal Muscle of a Presymptomatic Pompe Mouse Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarlena Rohm,Leon Volke,Lara Schlaffke,Robert Rehmann,Nicolina Südkamp,Andreas Roos,Anne Schänzer,Andreas Hentschel,Matthias VorgerdPubMed 37371072
- PloS one 17:e02774772022Rotenone causes mitochondrial dysfunction and prevents maturation in porcine oocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGeun Heo,Ming-Hong Sun,Wen-Jie Jiang,Xiao-Han Li,Song-Hee Lee,Jing Guo,Dongjie Zhou,Xiang-Shun CuiPubMed 36441709
- Nature communications 13:72122022Active DNA damage response signaling initiates and maintains meiotic sex chromosome inactivation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHironori Abe,Yu-Han Yeh,Yasuhisa Munakata,Kei-Ichiro Ishiguro,Paul R Andreassen,Satoshi H NamekawaPubMed 36443288
- Molecular genetics and metabolism reports 33:1009322022ITCH deficiency clinical phenotype expansion and mitochondrial dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRachel Wolfe,Paige Heiman,Olivia D'Annibale,Anuradha Karunanidhi,Alyssa Powers,Marianne Mcguire,Bianca Seminotti,Steven F Dobrowolski,Miguel Reyes-Múgica,Kathryn S Torok,Al-Walid Mohsen,Jerry Vockley,Lina Ghaloul-GonzalezPubMed 36338154
- Cell 185:3913-3930.e192022X-linked ubiquitin-specific peptidase 11 increases tauopathy vulnerability in women.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Yan,Xinming Wang,Dale Chaput,Min-Kyoo Shin,Yeojung Koh,Li Gan,Andrew A Pieper,Jung-A A Woo,David E KangPubMed 36198316
- The Journal of biological chemistry 298:1024762022Using reporters of different misfolded proteins reveals differential strategies in processing protein aggregates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKara L Schneider,Doryaneh Ahmadpour,Katharina S Keuenhof,Anna Maria Eisele-Bürger,Lisa Larsson Berglund,Frederik Eisele,Roja Babazadeh,Johanna L Höög,Thomas Nyström,Per O WidlundPubMed 36096201
- iScience 25:1045952022Role of the DUB enzyme USP7 in dendritic arborization, neuronal migration, and autistic-like behaviors in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Qiao,Yuan Tian,Yuda Huo,Heng-Ye ManPubMed 35800757
- Insect science 29:1251-12612022Smurf participates in Helicoverpa armigera diapause by regulating the transforming growth factor-β signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaiyin Li,Qin Lu,Yan Li,Yufang Yan,Zhiyong Yin,Jianjun Guo,Weihua XuPubMed 35064956
- Orphanet journal of rare diseases 17:292022Novel insights into PORCN mutations, associated phenotypes and pathophysiological aspects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnnabelle Arlt,Nicolai Kohlschmidt,Andreas Hentschel,Enrika Bartels,Claudia Groß,Ana Töpf,Pınar Edem,Nora Szabo,Albert Sickmann,Nancy Meyer,Ulrike Schara-Schmidt,Jarred Lau,Hanns Lochmüller,Rita Horvath,Yavuz Oktay,Andreas Roos,Semra HizPubMed 35101074
- Molecular medicine (Cambridge, Mass.) 27:1372021IκB kinase promotes Nrf2 ubiquitination and degradation by phosphorylating cylindromatosis, aggravating oxidative stress injury in obesity-related nephropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYin-Yin Chen,Han Hong,Yu-Ting Lei,Jia Zou,Yi-Ya Yang,Li-Yu HePubMed 34711178
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Native Full Length Protein corresponding to Cow UBB conjugated to Keyhole Limpet Haemocyanin. Database link P0CG53
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Saccharomyces cerevisiae | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
Species Mouse | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
UbiquitinExists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
Alternative names
Polyubiquitin-B, UBB
Recommended products
Rabbit Polyclonal UBB antibody. Suitable for WB and reacts with Human, Mouse, Saccharomyces cerevisiae samples. Cited in 60 publications. Immunogen corresponding to Native Full Length Protein corresponding to Cow UBB conjugated to Keyhole Limpet Haemocyanin.
Alternative names
Polyubiquitin-B, UBB
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Native Full Length Protein corresponding to Cow UBB conjugated to Keyhole Limpet Haemocyanin. Database link P0CG53
- Purification technique
Affinity purification Protein A
- Specificity
It can identify free ubiquitin as well as ubiquinated proteins. The antibody recognizes polyubiquitin chains more strongly than monoubiquitinated molecules.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ubiquitin is a small regulatory protein found in almost all tissues of eukaryotic organisms. It has a molecular weight of approximately 8.6 kilodaltons. It functions mechanically by attaching to proteins through a process called ubiquitination which involves the formation of an isopeptide bond. Ubiquitin molecules can form polyubiquitin chains through different lysine residues such as K48 and K63 that determine their function. These chains label substrate proteins for various fates including degradation. Ubiquitin is expressed ubiquitously in cells reflecting its essential role in maintaining protein homeostasis.
- Biological function summary
The ubiquitin system plays a critical role in regulating protein turnover and quality control within cells. It is part of a larger complex known as the ubiquitin-proteasome system (UPS) which is responsible for degrading proteins that need to be turned over. This process is essential for cell cycle control response to oxidative stress and DNA repair. Ubiquitin's role in tagging proteins for degradation or signaling allows cells to respond quickly to changes in their environment and maintain balance.
- Pathways
Ubiquitin functions in several important biological pathways including the Wnt and NF-kB pathways. In the Wnt signaling pathway ubiquitination modulates the stability of key components thereby affecting the pathway's overall activity. In the NF-kB signaling pathway ubiquitin labels inhibitor proteins for degradation which releases and activates NF-kB. These pathways highlight ubiquitin's interaction with proteins such as beta-catenin in Wnt and IkB in NF-kB illustrating how it regulates diverse cellular processes.
- Associated diseases and disorders
The dysfunction of the ubiquitin system is linked to neurodegenerative diseases and cancers. Ubiquitin-related defects in protein degradation can lead to the buildup of unwanted proteins contributing to conditions like Parkinson's disease. Connections with cancer are evident as ubiquitin controls cell cycle proteins and aberrant ubiquitination may drive tumor growth and progression. The protein p53 known to be controlled by ubiquitination plays a significant role in cancer related mechanisms when dysregulated. Understanding and targeting ubiquitin-related pathways may provide new therapeutic opportunities for treating these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Ubiquitin antibody (ab19247)
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. ab19247 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/200 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging using the Licor Odyssey CLx.
All lanes: Western blot - Anti-Ubiquitin antibody (ab19247) at 1/200 dilution
Lane 1: HeLa cell lysate (Control, untreated) at 20 µg
Lane 2: HeLa cells treated with MG132 (50 uM for 90 min) at 20 µg
Lane 3: Mouse Brain at 20 µg
Performed under reducing conditions.
Predicted band size: 26 kDa
Western blot - Anti-Ubiquitin antibody (ab19247)
All lanes: Western blot - Anti-Ubiquitin antibody (ab19247) at 1/1000 dilution
All lanes: Heat-shocked HeLa cell lysate
Predicted band size: 26 kDa
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